Effect of a combination of medium chain triglycerides, linoleic acid, soy lecithin and vitamins A and E on wound healing in rats

Abstract

Purpose:The aim of the study was to determine the effect of a combination of medium chain triglycerides (caprylic, capric, caproic and lauric acids), linoleic acid (essential fatty acid), vitamins A and E and soy lecithin, through a morphometric study, on the wound healing kinetics of experimental cutaneous ulcers. Methods: A total of 45 male Wistar rats were used, in which a skin flap of total thickness with an area of 4 cm 2 was removed. The animals were divided randomly into 3 groups of 15 rats each, Control, Reference and Test groups, which were treated topically with 0.9% NaCl, a preparation of clostebol combined with neomycin sulfate and the test formulation, respectively. The wound areas were measured by digital planimetry at days zero, 3, 7 and 12 postoperative. Based on the wound area, we determined the degree of tissue repair and mean rate of repair at different time intervals. Results: At day 3, an expansion of the wound area was observed in the Reference group and slight contraction in the Control and Test groups. On the subsequent days, the healing process, according to the degree of repair, proceeded in a linear manner, such that, at day 12, the healed area reached 77.95% of the initial ulcerated region in the Control group, 78.40% in the Reference group and 83.49% in the Test group, showing no significant differences. The overall mean rate of repair was equally similar at 12 days of treatment: 25.79 mm 2 /dia in the Control group, 25.42 mm 2 /dia in the Reference group and 27.38 mm 2 /dia in the Test group. Conclusion: The test preparation, applied topically on the experimentally induced cutaneous ulcers in rats, did not accelerate the process of tissue repair by secondary union. Key words: Triglycerides. Linoleic Acid. Wound Healing. Rats. RESUMO Objetivo:Avaliar o efeito da associação de triglicerídeos de cadeia média (ácidos caprílico, cáprico, capróico e láurico), ácido linoléico (ácido graxo essencial), vitaminas A e E e lecitina de soja, através de estudo morfométrico, na cinética de reparação de úlceras cutâneas experimentais. Métodos: Utilizaram-se 45 ratos, machos, da linhagem Wistar, nos quais foi removido um retalho cutâneo de espessura total com 4 cm 2 de área. Os animais foram divididos aleatoriamente em 3 grupos constituídos de 15 ratos, Controle, Referência e Teste, que foram tratados por via tópica respectivamente, com solução salina 0,9%, composto de clostebol associado a sulfato de neomicina e a formulação em teste. As áreas das feridas foram mensuradas por planimetria digital nos dias zero, 3, 7 e 12 de pós-operatório. A partir da área da ferida, calcularam-se ainda o grau de reparação e a taxa média de reparação em intervalo de tempo. Resultados: No 3o dia observou-se uma expansão da área da ferida no grupo referência e uma leve contração nos grupos controle e teste. Nos dias subseqüentes o processo de reparação, medido pela variável grau de reparação, evoluiu de forma linear, de modo que, no 12 o dia, a área reparada alcançou 77,95% da região ulcerada inicial no grupo...

Effects of topical application of pure and ozonized andiroba oil on experimentally induced wounds in horses

“…The choice of the size of the wounds was based on studies performed similarly in horses (MARTINS et al, 2003;SOUZA et al, 2006;, as well as depth of the lesions (MAGALHÃES et al, 2008; including skin and subcutaneous tissue, without involving adjacent muscles. Initial total area of the wounds was similar to those of the authors mentioned above, but differed in format to Oliveira , who solely conducted square wounds in similar experiment.…”

“…The wound area was estimated (MAGALHÃES et al, 2008) using the following equation: A = π x R x r (where A represents the area, R represents the greatest distance and r the shortest distance recorded in the wound).…”

Utilização dos ácidos graxos no tratamento de feridas: uma revisão integrativa da literatura nacional

“…A rat study (4) was conducted with the objective to evaluate the effect of the association of medium-chain triglycerides (caprylic, capric, capric and lauric acids), linoleic acid (essential fatty acid), vitamins A and E and soy lecithin (test product), by means of morphometric analysis, in 45 male Wistar rats, regarding the kinetics of repairing experimental skin ulcers. The animals were randomly assigned to 3 groups containing 15 rats each, Control group (saline solution 0.9%), Reference group (clostebol compound associated with neomycin sulphate) and Test group, which received daily topical therapy, from the 1 st to the 12 th PO day.…”

“…Because they are classified as medium-chain triglycerides, they are useful as a nutritional source, solvent, medium, and stabilizer of oral, topical or parenteral products. They can be used for the treatment, as well as the prevention of, ammoniacal dermatitis and pressure ulcers, forming a protective barrier on the skin and impeding maceration, besides being important in cellular inflammation processes, providing relief after the first application and boosting local cellular nutrition, in addition to having a great capacity for tissue regeneration (4) . All these components work in a way that increases the immune response, thus accelerating the inflammatory process and, consequently, stimulating the healing process through angiogenesis and epithelialization, facilitating the entrance of growth factors into the cell (5) .…”

Effect of gallium-arsenide laser, gallium-aluminum-arsenide laser and healing ointment on cutaneous wound healing in Wistar rats

“…Essential fatty acids such as those derived from linoleic acid are among the resources supporting the healing process (7). Dersani ® ointment belongs to this group, being used www.bjournal.com.br Braz J Med Biol Res 43(4) 2010 in any type of cutaneous lesion as a bactericide during the different phases of the process (3).…”