Evaluation of the macroscopic growth degree of experimental endometriosis in rats

Abstract

PURPOSE: To evaluate macroscopically the growth degree of self-transplantation of endometriosis in rats. METHODS: Forty female rats, after a 7-day period for adpating and evaluating of the estrous cycle regularity, underwent tail abdominal midline laparotomy with 3-cm cuts. The average third of the left uterine horn was removed, 4mm x 4mm patches in liquid environment were made, and self-transplanted in the rat mesenterium with a single stitch, and the endometrial surface of the endometriotic implant facing the lumen of the peritoneal cavity. The rats were programmed to die after three weeks. The abdominal cavity displaying was held and self-transplants were identified and classified. RESULTS: The results achieved were: one case for degree 0 (2,5%), three cases for degree 1 (7,5%), eleven cases for degree II (27,5%) and twenty-five cases for degree III (62,5%). CONCLUSION: The experimental endometriosis development, through the self-transplantation technique, showed to be most common in degrees 3 and 2 of development.

The effects of certain angioneogenesis inhibitors in experimental endometriosis in rats

“…The morphological data we have obtained coincide with those of other researchers who used a similar technique. Thus, Elgamal et al [65] describes ectopic endometrioid lesions macroscopically in the form of cysts filled with fluid, and the histological picture obtained by us completely corresponds to the described one by Rezende et al [66], Neto et al [67] and Amaral et al [68] (Fig. 1).…”

Ectopic uterine tissue as a chronic pain generator

“…Two weeks post-surgery, 100% of animals implanted in the gastrocnemius muscle with uterus tissue developed a cystic lesion, which contrasts with the lower rate of success reported for the induction of peritoneal endometriosis-like lesions (Cason et al, 2003; Nogueira Neto et al, 2007; Do Amaral et al, 2009). Particular characteristics of skeletal muscle as a tissue receptor might have contributed to the high rate of cystic lesion induction observed here.…”