Influence of nicotine on healing of vesical sutures in rabbits

Machado, Christiano; Chin, Eduardo Wei Kin; Ioshii, Sérgio Ossamu; Filho, Renato Tambara; Bathen, Aline Von

doi:10.1590/s0102-86502007000600012

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Scraping/scratching Culturing of cells in vitro followed by scraping/scratching Human, mouse [40][41][42][43] Punch Biopsy Culturing of cells in vitro followed by punch biopsy Human [44] In vivo surgery Surgery on live animals, such as cystotomy or partial cystectomy Rat, rabbit [35,36,45,46] In vivo over inflation Instillation of water in live animals' bladder causing over inflation, injuring the mucosa and injury to the submucosa and muscle to a lesser extent. Mouse [39] Application of a necrotizing, freeze-thaw Local application of a necrotizing, freeze-thaw injury to the serosal surface Rabbit [47] Table 2.…”

Section: Mechanism Of Injury Study Design Species Referencementioning

confidence: 99%

A Review on Bladder Wound Healing after Mechanical Injury

Larsson¹,

Chamorro²

2016

J Tissue Sci Eng

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Congenital defects of the urinary bladder that requires surgical intervention with mechanical wounding are common situations among pediatric urology patients. In conditions with severe lack of tissue, regenerative medicine with autologous cells has become a field of interest for future cure. In both situations, normal bladder wound healing is of major importance for an uneventful healing process and for the final results. Much effort has been put into increasing our understanding in the area of urinary bladder wound healing. Several methods have been used in different studies, all representing different clinical settings to address the issue of normal healing. However, little is known about the differences between these different wound-healing models. In this review, we aimed at summarizing what is known about the process of bladder wound healing after mechanical injury. We present the most commonly used methods in this area; describe the process of healing and the current knowledge on involved signaling transduction factors.

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Section: Mechanism Of Injury Study Design Species Referencementioning

confidence: 99%

A Review on Bladder Wound Healing after Mechanical Injury

Larsson¹,

Chamorro²

2016

J Tissue Sci Eng

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“…Nevertheless, there is no consensus on why the influence of nicotine reduces collagen synthesis. Furthermore, Machado (21), studying the healing of rabbit bladders, found no changes in collagen density in the scars. In the present study we found a reduced concentration of collagen of the foreskin in smokers, but without statistical significance.…”

Section: Resultsmentioning

confidence: 98%

Structural alterations of foreskin caused by chronic smoking may explain high levels of urethral reconstruction failure using foreskin flaps

et al. 2012

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ARTIcLE INFO _________________________________________________________ ___________________Objectives: The aim of the present study was to perform a stereological and biochemical analysis of the foreskin of smoker subjects. Materials and Methods: Foreskin samples were obtained from 20 young adults (mean = 27.2 years old) submitted to circumcision. Of the patients analyzed, one group (n = 10) had previous history of chronic smoking (a half pack to 3 packs per day for 3 to 13 years (mean = 5.8 ± 3.2). The control group included 10 nonsmoking patients. Masson's trichrome stain was used to quantify the foreskin vascular density. Weigert's resorcin-fucsin stain was used to assess the elastic system fibers and Picrosirius red stain was applied to study the collagen. Stereological analysis was performed using the Image J software to determine the volumetric densities. For biochemical analysis, the total collagen was determined as µg of hydroxyproline per mg of dry tissue. Means were compared using the unpaired t-test (p < 0.05). Results: Elastic system fibers of smokers was 42.5% higher than in the control group (p = 0.002). In contrast, smooth muscle fibers (p = 0.42) and vascular density (p = 0.16) did not show any significant variation. Qualitative analysis using Picrosirius red stain with polarized light evidenced the presence of type I and III collagen in the foreskin tissue, without significant difference between the groups. Total collagen concentration also did not differ significantly between smokers and non-smokers (73.1µg/mg ± 8.0 vs. 69.2µg/ mg ± 5.9, respectively, p = 0.23). Conclusions: The foreskin tissue of smoking patients had a significant increase of elastic system fibers. Elastic fibers play an important role in this tissue's turnover and this high concentration in smokers possibly causes high extensibility of the foreskin. The structural alterations in smokers' foreskins could possibly explain the poor results in smoking patients submitted to foreskin fasciocutaneous flaps in urethral reconstruction surgery.

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“…However, there is no consensus concerning the fact that under the influence of nicotine there is a reduction in collagen synthesis. Machado et al 20 , studying the healing of rabbit bladders, found no changes to collagen density in the scars.…”

Section: Discussionmentioning

confidence: 96%