Evaluation of an experimental model for anorectal anomalies induced by ethylenethiourea

Abstract

PURPOSE: To evaluate an experimental model for anorectal anomalies and their principal associated malformations induced by ethylene thiourea (ETU). METHODS: Rat fetuses were utilized, divided into two groups: experimental group - fetuses from rats that received ETU on the 11th day of gestation at the dose of 125 mg/kg, diluted in distilled water to 1% concentration (12.5 ml/kg); and control group - fetuses from rats that received distilled water alone, at a volume of 12.5 ml/kg. On the 21st day of gestation, the animals were sacrificed by hypoxia in a carbon dioxide chamber, followed by laparotomy to remove the fetuses. These were initially examined externally to determine the sex and whether anorectal anomalies and malformations of the vertebral column and tail were present. Then, with the aid of microscopy, the fetuses underwent exploratory laparotomy to characterize the type of anorectal anomaly and investigate urological malformations. RESULTS: None of the fetuses in the control group presented anorectal anomaly, vertebral column malformation or urological structural alterations. In the experimental group, 71% presented anorectal anomaly, 80% presented vertebral column alterations and 35% presented urological alterations. CONCLUSION: The model described was shown to be easy to implement and presented results that allow its use in studying anorectal anomalies and associated malformations.

Hepatic damage in newborns from female rats exposed to the pesticide derivative ethylenethiourea

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“…The effects of chronic intoxication have not been adequately characterized, since they may only become apparent after many years of exposure 2 . Nonetheless, both experimental and epidemiological evidence exists regarding damage to reproduction and gestation 6,7 . Despite improvements over the last few years, regarding the information available on pesticide residues in foods,…”

Study of the density of ganglion cells in the terminal bowel of rats with anorectal malformations

“…For neuroglial cells, S-100 protein and glial fi brillary acidic protein (GFAP) [14][15] reactions may be used; and for GC staining, neuron-specifi c enolase and neurofi lament protein reactions 14 . The aim of this research is the comparative study of GC density in the terminal intestine of normal rats and rats with ARM induced by ethylenethiourea (ETU) 16 , using S-100 protein reaction.…”

Is it possible folic acid reduce anorectal malformations ethylenethiourea induced in rats?

“…The thickness of anal stratified squamous epithelium (ASSE) and intestinal epithelium (IE) were analyzed ( The previous work of our group has induced 71% of ARMs in rats treated by ETU 8 . In this paper, 65% of ARMs were induced by ETU.…”