Use of mannanoligosacharides as an adjuvant treatment for gastrointestinal diseases and this effects on E.coli inactivated in dogs<A NAME="volta1"></A>

Abstract

Use of mannanoligosacharides as an adjuvant treatment for gastrointestinal diseases and this effects on E.coli inactivated in dogsUse of mannanoligosacharides as an adjuvant treatment for gastrointestinal diseases and this effects on E.coli inactivated in dogs 1 Uso de mananoligossacarídeos fosforilados como adjuvante no tratamento de doenças gastrointestinais e seu efeito na inativação da E. coli em cães ABSTRACT Purpose:To evaluate the effects of mannanoligosacharides on dogs showing gastroenteritis. Methods: Sixteen dogs, 2-6 month-old, distributed into two groups: T1 -animals with gastroenteritis receiving treatment for the disease + mannanoligosacharides (2 g/animal).; and T2 -animals with gastroenteritis receiving just treatment for the disease. The animals were randomly included in the sample and all of them were submitted to blood and feces collection for coproculture. In the treatment for gastroenteritis antibiotic, antihemetic, vermifuge, vitamins and sorotheraphy were used. The parameters evaluated were the numbers of leucocytes, neutrophils, lymphocytes and the presence of enteropathogenic bacteria in feces. Results: The mannanoligosacharides was effective in eliminating pathogenic E. coli in 85.71% of the animals, while in the no-treated group only 25% of the animals were negative to E. coli. Conclusion: The mannanoligosacharides is effective in the control of pathogenic E. coli and it can be indicated as an adjuvant treatment for gastroenteritis in dogs. Key words: Gastroenteritis. Escherichia coli. Probiotics. Dog Diseases. was confusing to scientists due to the diversity and complexity of these compounds. Nowadays, the carbohydrates and oligosaccharides have been used as nutritional modulators of the immune system. The carbohydrates are involved in all aspects of biological sciences. The wide range of possibilities available related to the structure and function of polysaccharides makes glycomics a science that study sugars and the structure of these sugars that are components of the cells. The phosphorylated mannanoligosaccharides are derived from a specific yeast strain (Sacharomyces cerevisiae strain 1026). These complex carbon hydrates are found mostly in the yeast cell wall, which is composed of more than 30% of mannanoligosaccharides. The cell wall is a complex matrix of protein and complex carbon hydrate that serves as a protective barrier around the cell and an interface between the cell and the extracellular surroundings. Thus, this complex matrix was developed with special characteristics to allow the communication with the extracellular surroundings. After analyzing the function of sugars in intracellular communication, it was demonstrated that the complex oligosaccharides such as the mannanoligosaccharides play fundamental roles in these interactions 1 . In many cases, bacterial infections occur due to the ability of the bacteria to recognize sugars present in the host cell surface and use specific receptors that allow them to attach, colonize, and, in the case of pathogens, cause di...

Feeding Dietary Mannan Oligosaccharides to Juvenile Nile Tilapia, Oreochromis niloticus, Has No Effect on Hematological Parameters and Showed Decreased Feed Consumption

“…Similar to our results, Welker et al (2007) did not find any difference in the hematology of channel catfish, Ictalurus punctatus , fed MOS‐supplemented diet at 2 g/kg, and Hisano et al (2007) using at least 2% dehydrated yeast (principal source of MOS) inclusion in Nile tilapia diet also found no effects on hematology. Dogs fed 2 g of MOS per animal (Gouveia et al 2006) and turkeys supplemented to 1 g MOS/kg diet (Çetin et al 2005) showed any differences in hematological parameters. Studies on the effects of dietary MOS on fish hematology are scarce and involve immunological parameters such as lysozyme concentration, antibody titer (Staykov et al 2007), and alternative complement pathway (Torrecillas et al 2007).…”

A review of 733 published trials on Bio-Mos®, a mannan oligosaccharide, and Actigen®, a second generation mannose rich fraction, on farm and companion animals.

“…MOS has been shown to reduce faecal E. coli and C. perfringens and tended to promote greater concentrations of beneficial Lactobacilli and Bifidobacteria spp. (Strickling et al, 2000;Swanson et al, 2002a;Grieshop et al, 2004;Gouveia et al, 2006). Older dogs tend to have reduced concentrations of Bifidobacteria spp.…”

Effect of Dietary Mannan Oligosaccharide and Lignin on Population and Characteristics of Probiotic Bacteria Isolated From Japanes Quails

“…On the other hand, pathogenic microbiota decreased in response to prebiotic supplementation. Escherichia coli was reported to decrease in response to prebiotic mannan oligosaccharidesupplementation (Gouveia et al, 2006;Swanson and Fahey, 2006;Middelbos et al, 2007). Also, other studies reported a reduction in E.coli count with mannan oligosaccharide supplementation (Xu et al, 2003;Zdunczyk et al, 2005;Baurhoo et al, 2007a,b).…”