Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats

Abstract

Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats 8 -ORIGINAL ARTICLE Proliferative effect of the aqueous extract of Hyptis pectinata on liver regeneration after partial hepatectomy in rats 1 Efeito do extrato aquoso da Hyptis pectinata na regeneração hepática após hepatectomia parcial em ratos ABSTRACT Purpose: This study was carried out to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver regeneration and on serum enzymes (AST, ALT and γ-GT) after 67% partial hepatectomy in rats. Methods: AST, ALT and γ-GT, were determined by conventional procedures using a spectrophotometer (Model E2250-CELM). Liver regeneration was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). Results:Oral pretreatment during 4 days at 100 mg/kg increased liver regeneration index. At 200 mg/kg, AST level was statistically decreased in comparison to the group submited to distilled water administration. The other enzymes assessed disclosed no difference when all groups were compared. Conclusion: The present study shows that the aqueous extract of Hyptis pectinata leaves contains some biological active principles that stimulate liver regeneration at 100 mg/kg and cause slight hepatic protection at 200 mg/kg. Key words: Liver Regeneration. Partial Hepatectomy. Hyptis pectinata. Plant. RESUMOObjetivo: Este estudo foi realizado com o objetivo de verificar o efeito do extrato aquoso da Hyptis pectinata na regeneração hepática bem como nos níveis das enzimas séricas (AST, ALT e γ-GT) após hepatectomia parcial de 67% em ratos. Métodos: AST, ALT e γ-GT, foram determinadas pelo método cinético utilizando um espectrofotômetro (Modelo E2250-CELM). A regeneração hepática foi avaliada por imunohistoquímica (PCNA). Resultados: O pré-tratamento oral de 100 mg/ kg foi realizado durante 4 dias e causou aumento na regeneração hepática O pré-tratamento oral com 200 mg/kg diminuiu significativamente os níveis de AST quando comparado com o grupo submetido ao pré-tratamento com água destilada. As demais enzimas avaliadas não apresentaram diferenças quando comparadas entre os grupos estudados. Conclusão: O presente estudo mostra que o extrato aquoso da Hyptis pectinata, numa concentração de 100 mg/kg possui alguma atividade biológica estimulando a regeneração hepática e causando também um leve efeito hepatoprotetor numa concentração de 200 mg/kg. Descritores: Regeneração Hepática. Hepatectomia Parcial. Hyptis pectinata. Planta.

Medicinal plants from the Yanesha (Peru): Evaluation of the leishmanicidal and antimalarial activity of selected extracts

“…have been shown to have anti-inflammatory (Grassi et al, 2006) and antiplasmodial properties (Chukwujekwu et al, 2005). Essential oils isolated from the genus Hyptis, have also been shown to have analgesic and regenerative properties (Melo et al, 2006;Santos et al, 2007;Menezes et al, 2007;Arrigoni-Blank et al, 2008). According to the wide range of activity of the genus, related to skin diseases, and the lack of references on Hyptis lacustris specifically, this plant should be considered for activity-guided fractionation studies.…”

Enhancement of Liver Regeneration by the Association of Hyptis pectinata with Laser Therapy

“…Statistical comparisons among the groups were performed by analysis of variance for parametric measurements with Bonferroni as posttest. (5). In that study we were able to test different concentrations of aqueous extracts of the plant, obtaining an optimal effect on hepatocyte proliferation at 100 mg/kg.…”

“…In experimental studies, it has been shown that an aqueous extract of Hyptis pectinata leaves protects the liver against injuries, stimulates its regeneration according to concentration, and has antinociceptive and antiedematogenic effects (3)(4)(5). Although the mechanism of biostimulation or inhibition remains obscure, some recent studies indicate that mitochondria may be the target organelle of the plants (6,7).…”

Synthesis and antibacterial activity of epoxide from hyptolide (Hyptis pectinata (L.) Poit) against Gram-positive and Gram-negative bacteria

“…The phytochemical content of Hyptis pectinata generally has a skeleton of α, β-unsaturated lactones, such as hyptolide compounds (Achmad et al, 1987), pectinolides A-C, sambacaitaric acid, rosmarinic acid (Falcao et al, 2013), pectinolides D-G (Boalino et al, 2003), and spicigerolide (Almtorp et al, 1991). The discovery of α, β-unsaturated lactones is an interesting study because of its potential effect as antinociceptive, antiedematogenic (Franzotti et al, 2001), antileishmanial (Falcao et al, 2013), antimalarial (Melo et al, 2006), antibacterial (Santos et al, 2008), and anticancer (Asy and Suzery, 2019;. The previous study reported that hyptolide has a basic framework of α, β-unsaturated C12-δ-lactones with a chemical formula, C 18 H 24 O 8 (Achmad et al, 1987).…”