Effects of enoxaparin on the healing of abdominal wall in rats: study on breaking strength and histopatology

Oliveira, Paulo Gonçalves de; Sousa, João Batista de; Reys, Luiz G.; Magalhães, Albino Verçosa de; Rabelo, Francieli de Sousa; Muniz, Karla Cardoso

doi:10.1590/s0102-86502006000400011

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…the use of enoxaparin caused an increase on the tensile breaking strength and worse collagen disposition at the 7 th postoperative day 15 . Experimental group was included significant loss of tensile strength in our study.…”

Section: Discussionmentioning

confidence: 97%

The effect of prophylactic dose of a low molecular weight heparin on skin wound healing of rats

et al. 2009

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Purpose:To investigate the effect of prophylactic dose of a low molecular weight heparin, enoxaparin, on skin wound healing of rats. Methods: Forty rats were used for the study. Rats were randomly assigned to two equal groups. Experimental group received prophylactic dose of enoxaparin. Physiologic saline was administered to the control group. Parameters of wound healing of experimental and control groups were compared. For comparison of the groups in terms of fibrosis, vascularization, inflammation, epithelization, and tensile strength test (Newton). Mann-Whitney-U test was used because variables were categorical data (fibrosis, vascularization, inflammation and epithelization). Differences between groups were analyzed with independent samples t-test (tensile strength). Significance was set at p<0.05. Results: Skin wound of the experimental group presented tensile strength significantly decreased (p<0.001), histopathologic examination revealed a significant (p<0.001) delayed epithelization and decreased in fibrosis, vascularization, inflammation (p<0.001) in the experimental group. Conclusion: Enoxaparin delay wound healing by decreased inflammatory cells, fibroblast contents and their products (growth factors), and by promoted hemorrhage.

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Section: Discussionmentioning

confidence: 97%

The effect of prophylactic dose of a low molecular weight heparin on skin wound healing of rats

et al. 2009

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“…The losses may have been associated with surgical stress 14 . Weight loss has been described in previous studies on wound healing 15,16 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

et al. 2012

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PURPOSE:To evaluate the effects of S-methylisothiourea hemisulfate (SMT) on the healing of colonic anastomosis in rats. METHODS:Sixty rats Wistar were distributed into two groups of 30 animals: experimental (E) and control C). The animals of experimental group received intraperitoneal SMT at 50mg/kg/dose every 12 hours for 72 hours. The control group received intraperitoneal saline at the same volume of SMT. The rats were subdivided into subgroups groups of 10 for euthanasia on the third, seventh, and 14 th postoperative days (POD). We evaluated clinical and weight evolution, breaking strength and histopathology; also, a blood sample was collected for serum dosage of nitrite/nitrate. RESULTS:There was more vascular neoformation (p=0.006) and granulation (p=0.002) in the E3 group, and more mononuclear infiltrates in the C3 group (p=0.041). There was also more edema in the C14 group (p=0.008). There was no statistically significant difference in breaking strength, nitrite/nitrate dosage, and the remaining histopathological parameters. CONCLUSION:The use of S-methylisothiourea hemisulfate improved the healing of colonic anastomosis in rats on the third postoperative day by accelerating the proliferative stage of healing, but without interfering with the breaking strength of the anastomosis. Key words: Nitric Oxide. Nitric Oxide Synthase. Wound Healing. Anastomosis, Surgical. Colon. Rats. RESUMO OBJETIVO:Avaliar os efeitos do hemissulfato de S-metilisotiouréia (SMT) na cicatrização de anastomoses colônicas em ratos no terceiro, sétimo e 14° dia de pós-operatório (DPO). MÉTODOS: Sessenta ratos Wistar foram distribuídos em dois grupos: experimental (E) e controle (C), com 30 animais cada. No grupoexperimental foi administrado SMT 50mg/kg/dose, intraperitoneal a cada 12 horas por 72 horas. O grupo controle recebeu NaCl a 0,9%.Os ratos foram subdivididos em grupos de 10 para eutanásia no terceiro, sétimo e 14° DPO. Avaliou-se a evolução clínica e o peso dos animais, a resistência tênsil e histopatologia da anastomose, e a dosagem de nitrito/nitrato no soro. RESULTADOS:Houve mais neoformação vascular (p=0,006) e de granulação (p=0,002) no grupo E3, e maior infiltração de mononucleares no grupo C3 (p=0,041). Houve também mais edema no grupo C14 (p=0,008). Não houve diferença estatisticamente significativa na resistência tênsil, a dosagem de nitrito / nitrato, e os restantes parâmetros histopatológicos. CONCLUSÃO:A utilização do hemissulfato de S-metilisotiouréia acelerou a cicatrização das anastomoses colônicas, a melhoria ocorreu no terceiro DPO: verificou-se que a fase proliferativa da cicatrização foi acelerada. Não houve interferência na resistência tênsil das anastomoses.Descritores: Óxido Nítrico. Oxido Nítrico Sintase. Cicatrização. Anastomose Cirúrgica. Colo. Ratos. Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

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“…The suture dehiscences both of intestinal anastomosis and of surgical wound complications are involved in the genesis of infections and increased mortality and morbidity rates, hospital permanence and high costs; therefore, factors that inhibit the inflammatory mediators may have undesirable effects on the process of repairing and healing of wounds 17,18,20 . Studies have reported that some drugs with antiinflammatory or pro-inflammatory action, including diclofenac sodium 8,13 , misoprostol 17 , alter the wound healing, interfering with inflammation and collagen deposition.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab

Lopes

Freitas²,

Marques

et al. 2008

Acta Cir. Bras.

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Purpose:To evaluate the effects of infliximab, a murine/human chimeric monoclonal antibody, on the tensile strength of abdominal wall surgical wounds. Methods: Sixty Wistar healthy male rats with initial body weight between 215 and 390 g and 60 and 90 days of age were randomly assigned into two groups, E (Experimental) and C (Control) with 30 animals each. Group E animals received a single subcutaneous dose of 5mg/Kg of infliximab, and Group C animals received equivalent subcutaneous volume of a solution of 0.9% NaCl. After 48h, animals from both groups were submitted to a 4 cm median incision in the abdominal wall, including all layers that had been reconstituted with continuous suture of the aponeurotic muscle and skin, with 5.0 nylon thread. Then, Group E animals were separated by simple allotment into three subgroups named E3, E7 and E14 with ten animals each, and those from group C into C3, C7, C14 and were submitted, respectively, the reoperation and euthanasia at the third, seventh and fourteenth postoperative day. The anterior abdominal wall, which was resected during reoperation, was cut with No 15 scalpel lamina perpendicularly to the surgical wound. Each specimen, in the form of a 6 cm x 2 cm strip, was fixed by the extremity so that the suture line was equidistant from the fixation points of the dynamometer, in order to undergo the tensile strength test. The dynamometer, which was gauged for each series of measures, was calibrated to apply velocity to the 25 mm/min rupture test; the rupture value was expressed in N (Newton). Prior to euthanasia, the abdominal vena cava was identified and punctured in order to collect blood for TNF-α dosage. Results: The mean tensile strength found for animals from subgroups E3, E7, E14, C3, C7, C14 were, respectively, 16. 03, 18.69, 27.01, 28.40, 27.22, 29.15 and 24.30 N. In the results of the multiple comparisons tests, significant differences (p<0.05) was found between subgroups E3 and E7 compared with C3, C7 and C14. Conclusion: The infliximab interfered in the healing of the abdominal wall wound decreasing the rupture strength in the inflammatory and proliferative phases. Key words: Antibodies, Monoclonal. Abdominal Wall. Wound Healing. Rats. RESUMOObjetivo: Avaliar os efeitos do infliximabe, anticorpo monoclonal quimérico humano-murino, sobre a força tênsil da ferida operatória abdominal. Métodos: Sessenta ratos, linhagem Wistar, machos, sadios, com peso corporal inicial entre 215 e 390 g e 60 e 90 dias de vida foram distribuídos aleatoriamente em dois grupos, E (Experimental) e C (Controle) com 30 animais cada. Os animais do grupo E receberam por via subcutânea, dose única, de 5mg/Kg de infliximabe, via subcutânea e os animais do grupo C receberam, volume equivalente, de solução de NaCl a 0,9%, via subcutânea. Depois de 48h os animais de ambos os grupos foram submetidos à incisão mediana na parede abdominal com 4 cm de extensão incluindo todos os planos que foram reconstituídos com sutura contínua músculo aponeurótica e pele, separadamente, com fio de...

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Effects of enoxaparin on the healing of abdominal wall in rats: study on breaking strength and histopatology

Cited by 4 publications

References 13 publications

The effect of prophylactic dose of a low molecular weight heparin on skin wound healing of rats

The effect of prophylactic dose of a low molecular weight heparin on skin wound healing of rats

Evaluation of the effects of S-methylisothiourea hemisulfate, an inducible nitric oxide synthase inhibitor, on the healing of colonic anastomosis in rats

Analysis of the tensile strength on the healing of the abdominal wall of rats treated with infliximab

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