Glycine reduces tissue lipid peroxidation in hypoxia-reoxygenation-induced necrotizing enterocolitis in rats

Meyer, Karine Furtado; Martins, José Luiz; Filho, Luiz Gonzaga de Freitas; Oliva, Maria Luíza Vilela; Patrı́cio, Francy Reis da Silva; Macedo, Maurício; Wang, Lina

doi:10.1590/s0102-86502006000300008

Cited by 12 publications

(8 citation statements)

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“…Our results in NEC group are consistent with prior studies reporting increased MDA levels implicating an increase in lipid peroxidation in experimental NEC models. [17][18][19] Research on both humans 5 and animals 12 revealed that QE is effective on preventing lipid peroxidation. Expectedly, intraperitoneal administration of 15 mg/kg QE resulted in a decrease in MDA levels in our study.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Quercetin on Necrotizing Enterocolitis in a Neonatal Rat Model

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Quercetin on Necrotizing Enterocolitis in a Neonatal Rat Model

et al. 2017

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“…In Brazil, Meyer et al. evaluated in the NEC model in rats, just the degree of intestinal involvement with hypoxia and reperfusion without using formula or hypothermia 8 .…”

Section: Introductionmentioning

confidence: 99%

Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure

et al. 2013

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PURPOSE: To describe the difficulties of implementing the protocol of experimental necrotizing enterocolitis (NEC) in order to obtain a larger number of newborns affected with the disease and a lower mortality. METHODS: Term Sprague-Dawley newborns rats (22 days) were divided into four groups of 12 fetuses each (n = 48): EC - breastfed newborns; IH - breastfed newborns and subjected to a stress protocol by ischemia and hypothermia; ESB - formula-fed newborns (Esbilac®, PetAg, Hampshire, IL, USA) and NEC - formula-fed newborns and subjected to stress protocol. The parameters set for the study protocol were: milk concentration (0.19 g ml or 0.34 g/ml), diet instilled volume (according to body weight - 200 kcal/day/Kg - or progressive, according to acceptance), weight (gain, loss or maintenance) and duration of the experiment (72 hours or 96 hours). Data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were obtained. Samples of terminal ileum were collected and analyzed by the degree of injury to the intestinal wall. Statistically significance was set to p<0.05. RESULTS: The established protocol with less mortality and increased number of NEC was with Esbilac® at a concentration of 0.19 g/ml of diet instilled volume of 0.1 ml, every 3 hours, for 72 hours. All infants fed with artificial milk lost weight. In the degree score of intestinal injury, the ESB, IH and NEC groups were considered positive for NEC with greater histological injury in the latter. CONCLUSION: The described NEC protocol in rats allowed a greater survival of puppies with a greater number of animals affected by the disease.

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“…On the other hand, there have been reports that, after 30 minutes of ischemia, there are tissue lesions visible under an optical microscope that are potentially reversible 6,7 . The full or partial participation of free radicals in microvascular lesions has been demonstrated, along with their participation in parenchymatous cells of tissue subjected to ischemia, through the hypoxia-reoxygenation process [8][9][10][11] . There are still doubts whether vascular lesions would or would not be accompanied by lesions of the intrinsic nervous system of the intestine 11 , especially with regard to the myenteric plexus (Auerbach) and submucous plexus (Meissner), with consequent modification of the intestinal motility.…”

Section: Introductionmentioning

confidence: 99%

“…The full or partial participation of free radicals in microvascular lesions has been demonstrated, along with their participation in parenchymatous cells of tissue subjected to ischemia, through the hypoxia-reoxygenation process [8][9][10][11] . There are still doubts whether vascular lesions would or would not be accompanied by lesions of the intrinsic nervous system of the intestine 11 , especially with regard to the myenteric plexus (Auerbach) and submucous plexus (Meissner), with consequent modification of the intestinal motility. Lesions of the nervous system have been described following ischemia/ reperfusion [12][13][14] , and many substances have been studied with a view to diminishing or avoiding these ischemia/reperfusion lesions 11,[15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

“…There are still doubts whether vascular lesions would or would not be accompanied by lesions of the intrinsic nervous system of the intestine 11 , especially with regard to the myenteric plexus (Auerbach) and submucous plexus (Meissner), with consequent modification of the intestinal motility. Lesions of the nervous system have been described following ischemia/ reperfusion [12][13][14] , and many substances have been studied with a view to diminishing or avoiding these ischemia/reperfusion lesions 11,[15][16][17] . However, it is not fully known what the characteristics of enteric nervous system lesions are, how they occur, and whether such damage is total or causes alterations to cell populations in the intestinal ganglia.…”

Section: Introductionmentioning

confidence: 99%

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Do lesions of the enteric nervous system occur following intestinal ischemia/reperfusion?

et al. 2007

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Purpose:To evaluate tissue lesions, especially those of the intestinal innervation, in an excluded jejunal loop subjected to ischemia and reperfusion in rats. Methods: To evaluate the role of ischemia and reperfusion lesions in an excluded intestinal loop, four groups of 20 rats were set up: control group (GCEI7) and three experimental groups (GIREI7, GIREI14 and GIREI28). They were all subjected to exclusion of an intestinal segment of six centimeters in length, at a distance of 10 centimeters from the Treitz angle. The 60 animals in the three experimental groups were additionally subjected to ischemia of the vascular pedicle for 30 minutes. The control group and the experimental group GIREI7 were evaluated on the 7 th day after the operation. The groups GIREI14 and GIREI28 (which also underwent ischemia) were utilized to evaluate the evolution of the lesion over time, on the 14 th and 28 th days after the operation, respectively. From the intestinal excluded loop, we take one ring of 0,5 cm distal and proximal, that were fixed in formaline 10% solution in order to do histological (HE) and immuno-hystochemial (PS-100) evaluation (enteric nervous system.) The distal loop was exteriorized in stoma and the proximal part closed with polipropilene 6-0. Results: It was observed a decrease in the number of ganglionic cells in the myenteric plexus in the group subjected to ischemia and reperfusion (GIREI7), in relation to the control group (GCEI7) at the 7 th post-operative day (Mann-Whitney test: p = 0.0173 *. Comparing the numbers of ganglionic cells in the myenteric plexus before and after jejunal loop exclusion GCEI7 -(Wilcoxon test: p = 0.0577). GIREI7 -Comparing the numbers of ganglionic cells in the myenteric plexus before and after ischemia (*p = 0.0399). Comparing the percentage variations in ganglionic cells in the myenteric plexus on the 7 th , 14 th and 28 th days after the procedure, in the groups GIREI7, GIREI14 and GIREI28, it was observed that there were no significant alterations. Kruskal-Wallis test: p = 0.6501. Conclusion: There was a decrease in the number of ganglionic cells in the myenteric plexus due to ischemia and reperfusion that did not recover in the late post-operative period. Key words: Intestine, small. Ischemia. Reperfusion. Enteric nervous system. RESUMO Objetivo: Avaliar lesões teciduais, especialmente aquelas da inervação intestinal em alça jejunal excluída submetida à isquemia e reperfusão em ratos. Métodos: Para avaliar o papel da isquemia e reperfusão nas lesões em uma alça intestinal exclusa, quatro grupos de 20 ratos foram criados: Grupo controle (GCE17) e 3 Grupos experimentais (GIRE!7, GIREI14) e GIREI28) Todos foram submetidos à exclusão de um segmento intestinal de seis centímetros de extensão, a 10 centímetros do ângulo de Treitz . Os 60 animais dos 3 grupos experimentais foram também submetidos a isquemia do pedículo vascular por 30 minutos.O grupo controle e o grupo experimental GIREI7 foram avaliados no 7º. Dia após a operação. Os grupos GIREI14 e GIREI28 também ...

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Glycine reduces tissue lipid peroxidation in hypoxia-reoxygenation-induced necrotizing enterocolitis in rats

Cited by 12 publications

References 28 publications

Protective Effects of Quercetin on Necrotizing Enterocolitis in a Neonatal Rat Model

Protective Effects of Quercetin on Necrotizing Enterocolitis in a Neonatal Rat Model

Validation of protocol of experimental necrotizing enterocolitis in rats and the pitfalls during the procedure

Do lesions of the enteric nervous system occur following intestinal ischemia/reperfusion?

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