Influence of arterial hypertension treated with losartan on skin healing in rats

Abstract

PURPOSE: To evaluate the role of Losartan in skin healing repair. METHODS: One hundred and eleven male Wistar rats were distributed into four groups, at random. Group I (n=31) underwent a laparotomy to induce hypertension by stenosis of the left renal artery, and 48 hours later it received Losartan (10 mg/Kg) daily. Group II (n=30) went through the same procedure and received isotonic saline solution. Group III (n=30) underwent a simulated laparotomy. Group IV (n=20) to confirm the induced hypertension method. Ketamine and Xylazin anesthesia was used in every painful/stressful procedure. After 15 days, skin healing repair was studied by a dorsal midline skin incision and second layer-skin incision in groups I, II and III, that was sutured in one layer with 4-0 nylon suture. Samples of the dorsal wall scar were taken 4, 7 and 14 days after the last procedure, and sent to strength and displacement analysis and histological preparation. RESULTS: By the 4th and 7th days of the analysis, group II scars showed to be less resistant than group III scars (p<0,05). The total amount of collagen was higher in group III on the three studied periods. The percentage occupied by total collagen in the wound area, on the 14th day, was lower in group I, due to its lower percentage of type I collagen CONCLUSION: Scars treated with Losartan were initially less resistant and had a lower collagen deposition.

Effects of antihypertensive drugs on surgical outcomes of breast reconstruction: a nationwide population-based claim study

“…We also conducted a retrospective clinical study on the effect of antihypertensive drugs on outcomes of breast reconstruction and found that ARB use was significantly associated with an increased risk of surgical complications after breast reconstruction (17). The findings of our previous clinical study regarding the effect of ARB use on surgical complications in the clinical setting were consistent with those of previous studies using experimental models (13)(14)(15)(16)28,29), despite the discrepancy in the dose and administration period of ARBs; the dose of ARBs was higher in experimental models (29,30), and chronic use of ARBs was predominant in human subjects (17).…”

“…ARBs act by selectively blocking angiotensin II type 1 receptor (AT1R), and clinical evidence suggests that they have cardiovascular, cerebral, and renal protective effects (27). In contrast, several experimental studies have demonstrated that angiotensin II-AT1R signaling plays a crucial role in wound healing and angiogenesis (26), and inhibition of AT1R by ARBs can cause decreased wound healing by inhibiting re-epithelialization, dermal repair, and angiogenesis (16) and reduced wound tensile strength by inhibiting collagen synthesis and deposition (15,28). In addition, treatment with ARBs has been demonstrated to cause massive graft loss after meshed split-thickness skin grafting in a porcine full-thickness burn wound model (29).…”

Impact of hypertension in liver regeneration in rats

“…After laparorrhaphy the animals were returned to their boxes where they were given water and food ad libitum until the date scheduled for hepatectomy. This method leads to hypertension as described in a previous study 13 . A week later, under new anesthesia, a new laparotomy was followed by partial hepatectomy, resection of the left lateral and median lobes.…”

Influence of arterial hypertension on colonic healing in rats

“…The proportion of type I collagen in Wistar rats on the fourth, seventh and fourteenth days after surgery for bowel suture, in the control group was 27.3%, 26.7% and 31.3%, respectively 18 .…”

“…Biondo-Simões et al 16,18 observed progressive gain of collagen in the anastomosis area on the fourth, seventh and fourteenth days after surgery for bowel suture in Wistar rats in the control group. Tostes et al 17 measured the concentration of hydroxyproline in the abdominal wall of SHR rats subjected to laparotomy, obtaining the progressive concentration of hydroxyproline, which is a good marker of the amount of collagen in tissue 36 , on the seventh and fourteenth day after surgery.…”