The role of ischemic preconditioning at the gracilis muscle of rats in the early phase of reperfusion injury

Webster, Ronaldo Scholze; Montero, E; Fagundes, Djalma José; Zettler, Cláudio Galleano; Coiro, José

doi:10.1590/s0102-86502006000200005

Cited by 5 publications

(4 citation statements)

References 16 publications

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“…According to studies using NAC, in different posologies and showing that it is effective on the I/R injury in organs such as kidney, liver, lung, fasciocutaneous flap and ovary 22,23 , NAC was chosen for the present study.…”

Section: Resultsmentioning

confidence: 99%

The Study of Estrous Cycle Phases Female Rats With the Use of N-Acetylcysteine in Ovarian Graft

Vieira

Gomes

Montero³

2021

J. Health Scie.

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Ovarian graft may be the target of the biochemical effects of oxidative stress caused at the time of transplantation. In order to evaluate the effect of N-acetylcysteine on the ovarian graft, regarding the estrous cycle preservation , 50 female and virgin EPM-1 Wistar rats, weighing up to 250g, originating from CEDEME of UNIFESP, were kept in adequate sanitary conditions. , receiving their own food and water. Daily vaginal smears were performed to identify the estrous phase for 8 days. The animals were randomly distributed into 05 groups: 1st Group (GTx), saline was administered subcutaneously, 2nd (NAC 150mgKg), 3rd (NAC 300mg / Kg), 4th (NAC 600mg / Kg) and 5th (NAC 1200mg / Kg) , that were administered NAC subcutaneously on the abdominal face, 60 minutes before left unilateral ovarian transplantation in retroperitoneum and contralateral oophorectomy for purposes of histomorphological analysis, with colpocytological evaluation. Euthanasia was performed by means of anesthetic lethal dose in half of the animals on the 4th postoperative day, with a single vaginal smear collection and euthanasia on the rest of the animals, between the 14th and 16th days, after the material was collected in order to define the estrus phase. It was evaluated in the graft that the animals exhibited in all groups return of estrous cycle in the later phase of the post-transplant, with better definition of regular cycle in the highest dosages of N-acetylcysteine. N-acetylcysteine induced the return of the estrous cycle in the rats’ ovarian graft , mainly in the highest dosage, proving its effectiveness in revascularization of the tissue after ischemia and reperfusion. Keywords: Acetylcysteine. Reperfusion. Histocompatibility Antigens. Menstrual Cycle. ResumoO enxerto ovariano pode ser alvo dos efeitos bioquímicos do stress oxidativo causado no momento do transplante. Com o objetivo de avaliar o efeito da N-acetilcisteína no enxerto ovariano, quanto à preservação do ciclo estral, foram utilizados 50 ratos EPM-1 Wistar, fêmeas e virgens, pesando até 250g, originários do CEDEME da UNIFESP, mantidos em adequadas condições sanitárias, recebendo ração própria e água. Realizados esfregaços vaginais diários para identificação da fase estral durante 08 dias. Os animais foram distribuídos aleatoriamente em 05 grupos: 1º Grupo (GTx), administrada solução salina via subcutânea, 2º (NAC 150mgKg), 3º (NAC 300mg/Kg), 4º (NAC 600mg/Kg) e 5º (NAC 1200mg/Kg), aos quais foi administrada NAC por via subcutânea em face abdominal, 60 minutos antes do transplante unilateral esquerdo do ovário em retroperitônio e à ooforectomia contra-lateral para fins de análise histomorfológica, com avaliação colpocitológica. A eutanásia foi realizada por meio da dose letal do anestésico em metade dos animais no 4º dia de pós-operatório, realizado única coleta de esfregaço vaginal e a eutanásia no restante dos animais, entre o 14 º e 16º dia, após a coleta do material para definição da fase estro. Foi avaliado no enxerto que os animais apresentaram em todos os grupos retorno de ciclo estral na fase mais tardia do pós-transplante, com melhor definição de ciclo regular nas dosagens mais elevadas de N-acetilcisteína. A N-acetilcisteína induziu o retorno do ciclo estral no enxerto ovariano de ratas, principalmente na maior dosagem comprovando sua eficácia na revascularização do tecido após isquemia e reperfusão. Palavras-chave: Acetilcisteína. Reperfusão. Transplante. Ciclo Ovariano.

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Section: Resultsmentioning

confidence: 99%

The Study of Estrous Cycle Phases Female Rats With the Use of N-Acetylcysteine in Ovarian Graft

Vieira

Gomes

Montero³

2021

J. Health Scie.

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“…[3][4][5] Bunun yanında iskelet kasındaki post-iskemik kontraktilite bozukluğunu azaltan faydalı etkileri olduğu, inflamatuvar reaksiyonu ve lökosit aracılı inflamasyon yanıtını azalttığı da gösterilmiştir. [3,[21][22][23] Reaktif oksijen radikalleri, İ/R hasarının patogenezinde temel bir rol oynamaktadır. Paradoksal olarak ROR'nin İÖK'yi ortaya çıkaran uyarının oluşmasında da görev aldıkları bilinmektedir.…”

Section: Malondialdehit öLçüm Yöntemiunclassified

Ischemic preconditioning and N-acetylcysteine in a rat model of skeletal muscle ischemia-reperfusion: does antioxidant therapy have an impact on ischemic preconditioning?

Gürcün¹

2012

Turk Gogus Kalp Dama

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Bu çalışmada iskemik ön koşullamanın (İÖK) ve N-asetilsisteinin (NAC) iskemi-reperfüzyon (İ/R) hasarı üzerine olan etkileri ve İÖK ile eş zamanlı olarak reaktif oksijen radikallerinin (ROR) tutucusu bir ajan olan NAC verilmesinin etkileri araştırıldı. Ça lış ma pla nı: Yirmi sekiz adet 230-255 gr Sprague-Dawley cinsi erkek sıçan, her grupta yedi sıçan olacak ve biri kontrol grubu olacak şekilde, dört gruba ayrıldı. Kontrol grubundaki sıçanlarda infrarenal abdominal aort 120 dakika boyunca klempe edilerek alt ekstremitelerde iskemi, ardından 50 dakika süreyle reperfüzyon gerçekleştirildi. İÖK grubunda, İ/R öncesinde üç siklus şeklinde 10'ar dakikalık iskemi ve reperfüzyon uygulandı. NAC grubunda iskemik periyodun sonunda 20 mg/kg NAC intravenöz bolus olarak verildi ve reperfüzyon süresince 20 mg/kg/saat dozunda idamesi sağlandı. İÖK+NAC grubunda, İÖK sırasında aynı dozda ve eşit sürede NAC uygulandı. Tüm gruplarda reperfüzyon süresinin sonunda doku örnekleri alınarak, sıçanlar sakrifiye edildi. Bul gu lar: Tüm çalışma gruplarında miyeloperoksidaz (MPO) ve malondialdehit (MDA) düzeyleri kontrol grubuna göre düşük bulundu. İndirgenmiş glutatyon (GSH) düzeyinin NAC grubunda diğer gruplardan yüksek olduğu saptandı. Çalışılan parametreler açısından İÖK ile İÖK+NAC grupları arasında anlamlı bir fark bulunmadı. Ancak İÖK grubunda MDA ve MPO düzeyinin daha düşük, GSH düzeyinin daha yüksek olduğu görüldü. So nuç:Deneysel İ/R hasarı modelinde İÖK ve NAC, sıçan iskelet kasındaki doku hasarlanmasını azaltmaktadır. İÖK sırasında ROR tutucu NAC verilmesi, İÖK'nin koruyucu etkisini tamamen ortadan kaldırmamakla birlikte bu etkide kısmi bir azalmaya neden olmuştur. Bu bulgu iskelet kasındaki İÖK oluşumunda ROR dışında bir takım mekanizmaların da etkisi olabileceğini düşündürmektedir. Anah tar söz cük ler: İskemi-reperfüzyon; iskemik ön koşullama; N-asetilsistein; reaktif oksijen radikalleri.

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“…It has been shown that Ischemic Preconditioning (IP) confers natural tissue protection to prolonged ischemic stress, involving multiple mediators of ischemic damage such as adenosine, norepinephrine, bradykinin, and unidentified opioids 7 . IP in skeletal muscle has showed a protective effect in vascular reperfusion, cellular membrane structure and function, muscle preservation, decreasing inflammatory infiltrate and vascular stasis [8][9][10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats

et al. 2014

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PURPOSE:To evaluate effects of ischemic preconditioning and Cilostazol on muscle ischemia-reperfusion injury. METHODS:Male Wistar rats were submitted to muscle ischemic and reperfusion injury (4h of the left common iliac artery occlusion followed by 1h of reperfusion). Five experimental groups were constituted: Control group (n=4); Ischemia-Reperfusion (IR, n=5); Ischemic preconditioning group (IP, n=6); Ischemia-Reperfusion group treated with cilostazol (IRCi, n=6) and Ischemic preconditioning group treated with cilostazol (IPCi, n=6). At the end, left gracile muscle was removed and embedded in paraffin. Histopathology, neutrophil infiltration, myocyte necrosis and edema were analyzed. RESULTS:When compared with the control group, IR group showed increased neutrophil infiltration, severe necrosis and edema. There was significant difference between myocytes necrosis of IR group and IP group. There was no difference between the histopathological changes between IP, IRCi and IPCi groups. CONCLUSIONS:The model of IR caused severe muscle injury in the rat hind limb and ischemic preconditioning has a protective effect, reducing myocyte necrosis, however, treatment with cilostazol and also the association between cilostazol and preconditioning has no protective effect on the skeletal muscle subjected to ischemia and reperfusion injury.

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The role of ischemic preconditioning at the gracilis muscle of rats in the early phase of reperfusion injury

Abstract: The IPC showed, in an earlier phase, a beneficial role at I/R derived injury on gracilis muscle of rats, as proven for the largest preservation of the fibers muscular, smaller inflammatory reaction and smaller vascular stasis.

Cited by 5 publications

References 16 publications

The Study of Estrous Cycle Phases Female Rats With the Use of N-Acetylcysteine in Ovarian Graft

The Study of Estrous Cycle Phases Female Rats With the Use of N-Acetylcysteine in Ovarian Graft

Ischemic preconditioning and N-acetylcysteine in a rat model of skeletal muscle ischemia-reperfusion: does antioxidant therapy have an impact on ischemic preconditioning?

Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats

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