Ischemia and reperfusion in skin flaps: effects of mannitol and vitamin C in reducing necrosis area in a rat experimental model

Yoshida, Winston Bonetti; Campos, Eduardo Zapaterra

doi:10.1590/s0102-86502005000500004

Cited by 28 publications

(7 citation statements)

References 34 publications

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“…They determined higher percentage of flap survival in ascorbic acid group. On the other hand, according to Yoshida and Campos [45], vitamin c and mannitol (antioxidant group) group did not prevent or reduce the necrosis area compared with control group in rat groin flap I/R injury model.…”

Section: Ascorbic Acid (Vitamin C)mentioning

confidence: 89%

The Effect of Antioxidants on Ischemia-Reperfusion Injury in Flap Surgery

Bozkurt¹,

Sezgiç²,

Karakol³

et al. 2019

Antioxidants

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Flap surgery has wide use in plastic surgery in the closure of tissue defects. In spite of the major advances in plastic surgery in the past years, flap surgery is still associated with significant mortality. Ischemia-reperfusion (I/R) injury, which is a complex injury associated with flap blood flow, is one of the most important causes of flap failure. The main pathophysiology underneath I/R injury is associated with reactive oxygen species, which can be prevented by certain antioxidant applications. Antioxidants have been widely used in flap surgery and I/R injury previously. There have been a lot of articles showing positive effects of antioxidants on I/R injury. In this chapter, we focus the mechanism of I/R injury and how antioxidants can able to diminish the damage, moreover demonstrating the effect of certain antioxidants on I/R injury that has been investigated previously.

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Section: Ascorbic Acid (Vitamin C)mentioning

confidence: 89%

The Effect of Antioxidants on Ischemia-Reperfusion Injury in Flap Surgery

Bozkurt¹,

Sezgiç²,

Karakol³

et al. 2019

Antioxidants

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“…The association of mannitol with Vitamin C reduced, but did not prevent, the occurrence of necrosis 18 . Iontophoresis, as a way of administration of hydralazine hydrochloride, was not effective to increase the viability of the flap, but when associated with histamine by the same route, there was increased viability 19 , the same occurring with minoxidil 20 .…”

Section: Dias Groupsmentioning

confidence: 92%

Ação da l-arginina na evolução de retalhos cutâneos de ratos sob exposição à nicotina

Guimarães

Moreira

Rocha

et al. 2013

Rev. Col. Bras. Cir.

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OBJETIVO: Avaliar se o tratamento com L-arginina influencia a cicatrização de retalhos cutâneos em ratos expostos à nicotina. MÉTODOS: Foram utilizados 40 ratos Wistar pesando 142,4±10,1g separados em quatro grupos: GC- tratamento com solução tampão fosfatos pH 7,4, confecção de retalho cutâneo, observação por 10 dias; GN- exposição à nicotina por quatro semanas, confecção de retalho cutâneo, observação por dez dias; GA- tratamento com solução tampão fosfatos pH 7,4 por quatro semanas, confecção de retalho cutâneo, tratamento com arginina por dez dias; GAN- exposição à nicotina por quatro semanas, confecção de retalho cutâneo, tratamento com arginina por dez dias. Foram avaliadas as áreas de necrose, re-epitelização, reação inflamatória e formação de tecido de granulação, pela coloração HE, a área de deposição total e a diferenciação de colágenos I e III por histometria com a coloração de picrosirius, e, através da marcação imunoistoquímica com anticorpo monoclonal anti-CD34, a densidade vascular cicatricial. RESULTADOS: As porcentagens de áreas de necrose de GN e GNA foram maiores (p<0,001) do que GC e GA. Nos escores histológicos, a deposição de colágeno e a porcentagem de colágeno tipo I, no GC e GA foram similares (p>0,05) e maiores (p<0,001) do que em GA e em GNA e, nas densidades vasculares, GN e GAN foram menores (p<0,001) do que em GC e em GA. CONCLUSÃO: A exposição à nicotina inibiu os efeitos da arginina, e nos ratos não expostos, induziu melhora na angiogênese e na deposição de colágeno total nos retalhos cutâneos.

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“…Two weeks after graft placement, the wound was examined to determine the area of necrosis. The percentage of graft survival was determined using the paper template technique [Yoshida and Campos, 2005]. Graft take percentage was quantified as follows:…”

Section: Transplantation Of the Composite Skinmentioning

confidence: 99%

A New System for Cultivation of Human Keratinocytes on Acellular Dermal Matrix Substitute with the Use of Human Fibroblast Feeder Layer

Xiao

Zhu²,

Ma³

et al. 2007

Cells Tissues Organs

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To improve the proliferative potential of human keratinocytes (HK) cultured on acellular dermal matrix (ADM), HK and mitomycin C-treated human fibroblasts (MMC-HF) were seeded onto ADM to form four types of composite skin: type I, HK were seeded onto the epidermal side of ADM; type II, both HK and MMC-HF were seeded onto the epidermal side; type III, MMC-HF were preseeded onto the dermal side of ADM, and then HK were seeded onto the epidermal side, and type IV, where MMC-HF were preseeded onto both sides, and then HK were seeded onto the epidermal side. Compared with type I and III, the proliferative potential of HK of type II and IV was significantly higher on day 3, 5, 7 and 9 in vitro. In type I and III, HK grew into one layer on day 7–9, while in type II and IV keratinocytes grew into a confluent monolayer by day 4–6. The adherence to ADM of HK in types II and IV was stronger than that in type I and III. The take rate of type II and IV composite skin was also significantly higher. In conclusion, when MMC-HF and HK were cocultured on the epidermal side of ADM, MMC-HF could serve as excellent feeder cells.

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Ischemia and reperfusion in skin flaps: effects of mannitol and vitamin C in reducing necrosis area in a rat experimental model

Abstract: The experimental model provided consistent necrotic area in control groups and drugs used were not effective in improving skin flap survival.

Cited by 28 publications

References 34 publications

The Effect of Antioxidants on Ischemia-Reperfusion Injury in Flap Surgery

The Effect of Antioxidants on Ischemia-Reperfusion Injury in Flap Surgery

Ação da l-arginina na evolução de retalhos cutâneos de ratos sob exposição à nicotina

A New System for Cultivation of Human Keratinocytes on Acellular Dermal Matrix Substitute with the Use of Human Fibroblast Feeder Layer

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