Viability of a random pattern dorsal skin flap, in diabetic rats

Abstract

The random pattern dorsal skin flap was less viable in the diabetic rats.

A Random-Pattern Skin-Flap Model in Streptozotocin Diabetic Rats

“…After our study was presented, some articles were published about flap viability in diabetic rats, but none of them explores optimum experimental duration in short-term diabetes. 15,16 In this study we preferred the application of a single dose of intraperitoneal streptozotocin (55 mg/kg), but there are many alternative ways to obtain diabetic animals, including viruses (encephalomyocarditis, Coxsackie B4, mengovirus), transgenic mice, and chemical toxins (alloxan, streptozotocin, zinc chelators).…”

The Influence of Diabetes Mellitus on Survival of Abdominal Perforator Flaps: An Experimental Study in Rats with Slowly Induced Diabetes Mellitus

“…We used fasting glycemia measurements because in our opinion this represents a more reproducible method for diabetes diagnosis. Even though pathophysiologically our method does not differ in comparison to other studies, 21,23,24 the fact that diabetes developed over a longer time period might be an advantage compared with the previously mentioned studies, which have recreated the diabetic model more abruptly, and the adaptation of the organism to the new metabolic stress was deficient. That is how we explain the good results we achieved regarding rat and flap survival rates (►Fig.…”

“…18 Afterward, further studies tried to address typical pathological changes in the small vessels of the skin and muscle, 12,[19][20][21] with the aim of determining the time point of the appearance of chronic vascular changes. This led to the development of cutaneous flap models in the diabetic rat [21][22][23][24] and determination of optimal surgical time point for the study of chronic vascular diabetic pathology. The optimal time point is essential to achieve accurate conclusions regarding the efficiency of flap surgery in diabetics.…”

“…In the majority of studies, 16,[25][26][27][28][29] diabetes induction methods included the administration of streptozotocin in doses varying between 60 and 70 mg/kg body weight with a resulting fast diabetes onset and high mortality rate during induction. Our group's experience with slow diabetic induction with doses of 50 mg/kg, 30,31 which leads to reduced induction mortality combined with previous experimental flap models in the diabetic rat, [21][22][23][24]32 led us to the idea of researching the survival of perforator flaps in the diabetic rat to try and assess the role of this surgical method for the diabetic patient. By using this lower streptozotocin dosage, a diabetic model is developed with partial destruction of pancreatic β cells (►Fig.…”

“…Although streptozotocin-induced diabetes does not perfectly reproduce the complex human diabetic disease, it represents a well-studied model for vascular diabetic complications. [19][20][21][22][23]26,29 Although it seems that clinical reconstruction in the diabetic patients is definitely worthwhile, there is only scarce and often contradictory experimental data [21][22][23][24]32 relating to flap reconstructions in patients with diabetes. Especially with respect to perforator flaps and diabetes, only a few studies with small animal populations have been undertaken.…”