Morphological and biomechanical study of abdominal aorta of rats submitted to experimental chronic alcoholism

Cerqueira, Nereide Freire; Yoshida, Winston Bonetti; Müller, Sérgio Swain; Sequeira, Júlio Lopes; Rodrigues, Antônio de Castro; Padovani, Carlos Roberto

doi:10.1590/s0102-86502005000300004

Cited by 13 publications

(8 citation statements)

References 21 publications

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“…In vitro studies have demonstrated that oxidized LDL stimulates the synthesis of collagen by vascular SMCs. 34 This could be explained by the fact that collagen fibers increase their cross linking and render them resistance to collagenases. This explains the prominence of collagen fibers in aging aorta, the increase of collagen/elastin ratio, as well as the progressive rigidity of the vessels wall.…”

Section: Discussionmentioning

confidence: 99%

The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia

Safi¹

2015

IJCEMS

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Background: hypercholesterolemia is a predominant risk factor for atherosclerosis. The clinical complications of atherosclerosis could be suppressed when plasma lipids are lowered by hypocholesterolemic agents. The aim of this research is to assess the possible protective effect of the drug Inegy versus cinnamon oil on the histological structure of the abdominal aorta of rats with experimentally induced hyperlipidemia. Materials & Methods: the animals were divided into 2 groups (control and experimental group). Group I (control group) was divided into: subgroup I a; received standard diet only, subgroup Ib received Inegy 10 mg/kg body weight / day orally and subgroup Ic received cinnamon oil orally. Group II (The experimental group) was divided into: subgroup II, which received a high fat high cholesterol (HFHC) diet, subgroup II b received simultaneously both HFHC diet and Inegy and subgroup II c which received simultaneously both HFHC diet and cinnamon oil. After 3 months blood samples were collected from all rats for cholesterol and triglyceride estimation and fresh specimens were taken from the abdominal aorta of each rat and processed for; light microscopic, immunohistochemical and morphometric studies. Results: Significant increase of serum cholesterol and triglyceride levels in hyperlipidemic rats (subgroup II a) was detected. Moreover, evident structural changes in the form of endothelial irregularity, focal loss and shedding of large areas of denuded endothelium were illustrated. It was associated with adhesion of mononuclear leukocytes and increased foam macrophages. Examination of histological sections of the abdominal aorta of (subgroups II b and II c) showed pronounced amelioration of the structural changes depicted in the aorta of the hyperlipidemic subgroup. Conclusion: HFHC diet induced evident biochemical, histological and immunohistochemical changes in the rat aorta. It was also concluded that both Inegy and cinnamon oil offered good protection against the histopathological changes induced by HFHC diet. There were no statistical significant differences between the protective effect of both agents as regards the biochemical and morphometric results.

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Section: Discussionmentioning

confidence: 99%

The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia

Safi¹

2015

IJCEMS

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“…The factorial design process led to the preparation of an optimized vascular graft spun from a 9% PCL solution containing 0.5% of PTX. It should be noted that the tensile stress values of the drug-loaded grafts after ␥-sterilization were always above 2 MPa, which is the maximum stress value for the natural vessels of rats and pigs 16,17 and is considered as a lower limit value for implanted synthetic grafts into the systemic circulation.…”

Section: Discussionmentioning

confidence: 99%

Paclitaxel-Eluting Biodegradable Synthetic Vascular Prostheses

et al. 2009

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Background-Clinical small-caliber vascular prostheses are unsatisfactory. Reasons for failure are early thrombosis and late intimal hyperplasia. We thus prepared biodegradable small-caliber vascular prostheses using electrospun polycaprolactone (PCL) with slow-releasing paclitaxel (PTX), an antiproliferative drug. Methods and Results-PCL solutions containing PTX were used to prepare nonwoven nanofibre-based 2-mm ID prostheses. Mechanical morphological properties and drug loading, distribution, and release were studied in vitro. Infrarenal abdominal aortic replacement was carried out with nondrug-loaded and drug-loaded prostheses in 18 rats and followed for 6 months. Patency, stenosis, tissue reaction, and drug effect on endothelialization, vascular remodeling, and neointima formation were studied in vivo. In vitro prostheses showed controlled morphology mimicking extracellular matrix with mechanical properties similar to those of native vessels. PTX-loaded grafts with suitable mechanical properties and controlled drug-release were obtained by factorial design. In vivo, both groups showed 100% patency, no stenosis, and no aneurysmal dilatation. Endothelial coverage and cell ingrowth were significantly reduced at 3 weeks and delayed at 12 and 24 weeks in PTX grafts, but as envisioned, neointima formation was significantly reduced in these grafts at 12 weeks and delayed at 6 months. Conclusions-Biodegradable, electrospun, nanofibre, polycaprolactone prostheses are promising because in vitro they maintain their mechanical properties (regardless of PTX loading), and in vivo show good patency, reendothelialize, and remodel with autologous cells. PTX loading delays endothelialization and cellular ingrowth. Conversely, it reduces neointima formation until the end point of our study and thus may be an interesting option for small caliber vascular grafts.

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“…0.018þF/3700 KN and operates in conjunction with a microcomputer using Mtest s 1.00 software. The velocity chosen for tension application was 30 mm/min [22,23]. At the end of the test, the software provided a load-elongation diagram and the results of yield point (N), stiffness coefficient (N/mm) and failure load (N).…”

Section: Biomechanical Characterizationmentioning

confidence: 99%

Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds

Bertanha

Moroz

Jaldin

et al. 2014

Experimental Cell Research

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Clinical experience for peripheral arterial disease treatment shows poor results when synthetic grafts are used to approach infrapopliteal arterial segments. However, tissue engineering may be an option to yield surrogate biocompatible neovessels. Thus, biological decellularized scaffolds could provide natural tissue architecture to use in tissue engineering, when the absence of ideal autologous veins reduces surgical options. The goal of this study was to evaluate different chemical induced decellularization protocols of the inferior vena cava of rabbits. They were decellularized with Triton X100 (TX100), sodium dodecyl sulfate (SDS) or sodium deoxycholate (DS). Afterwards, we assessed the remaining extracellular matrix (ECM) integrity, residual toxicity and the biomechanical resistance of the scaffolds. Our results showed that TX100 was not effective to remove the cells, while protocols using SDS 1% for 2h and DS 2% for 1h, efficiently removed the cells and were better characterized. These scaffolds preserved the original organization of ECM. In addition, the residual toxicity assessment did not reveal statistically significant changes while decellularized scaffolds retained the equivalent biomechanical properties when compared with the control. Our results concluded that protocols using SDS and DS were effective at obtaining decellularized scaffolds, which may be useful for blood vessel tissue engineering.

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Morphological and biomechanical study of abdominal aorta of rats submitted to experimental chronic alcoholism

Abstract: Chronic alcoholism did not cause morphological alterations in the aortic wall but increased elongation, without modifying any other mechanical properties.

Cited by 13 publications

References 21 publications

The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia

The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia

Paclitaxel-Eluting Biodegradable Synthetic Vascular Prostheses

Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds

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