Estudo funcional e morfológico da hipotermia sistêmica ou tópica no fígado de cães

Siqueira, Venilton José; Taha, Murched Omar; Fagundes, Djalma José; Gomes, Paulo de Oliveira; Juliano, Yára; Bruzzadelli, Renata Marcon Zanelatto; Caputto, Lucélia Rita Galdino

doi:10.1590/s0102-86502005000200015

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…This fact has been observed in the study by Siqueira et al 15 showing marked change in behavior in observation times. Failing to have statistical analysis of this group, these results were not considered relevant for this search.…”

Section: Discussionsupporting

confidence: 74%

Uso de soluções hipertônicas para preservação hepática em ratos

Yamaki

Teixeira

Brito

2013

ABCD, arq. bras. cir. dig.

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-Background -The success of a transplant depends mainly on the viability of the graft, which is currently the main point of difficulty focuses on the triad preservation-rejection-infection. There are several specific components of preservation solutions that could prevent certain tissue damage. From these components, the osmotic factor has been highlighted as a factor in preventing edema and subsequent cell death, suggesting a possible advantage in the use of hypertonic solutions for organ preservation. Aim -To compare different hypertonic solutions as alternative to liver preservation. Method -A total of 105 Wistar rats were divided in Standard Group (GP, n=5 rats), to verify the normal range of the study, and five experimental groups of 20 rats each, according to the preservation solution used: Group Eurocollins (GE), Group Saline 0.9% (GF), Group Glucose 50% (GG), Group Mannitol 20% (GM), Group Salty -NaCl 7.5% (GS). All animals in experimental group were also divided into four subgroups according to the time of collection in: 0 h, 2 h, 6 h and 12 h. Was assessed cell viability by the reaction with Methyl Blue Thiazolyl (MTT) and the dosages of lactate and alanine aminotransferase (ALT). Results -Regarding the lactate level, was observed a relative improvement of hypertonic solutions compared to eurocollins, and in 12 h, the GE and GS showed no statistically significant difference (p> 0.05). When assessed cell viability, absorbance at MTT also demonstrated favorable results to the GS, since no statistically significant difference in relation to GE. Conclusion -The 7.5% NaCl solution showed promising results for organ preservation, presenting parameters and capability comparable to eurocollins preservation solution.RESUMO -Racional -O sucesso de um transplante depende, principalmente, da viabilidade do enxerto, sendo que, atualmente, o maior ponto de dificuldade concentra-se na tríade preservação-rejeição-infecção. Existem diversos componentes específicos das soluções de preservação que previnem danos. Destes componentes o fator osmótico tem se destacado como de prevenção do edema e posterior morte celular, sugerindo possível vantagem na utilização de soluções hipertônicas para preservação de órgãos. Objetivo -Comparar soluções hipertônicas como alternativas para preservação hepática. Método -Foram utilizados 105 ratas Wistar distribuídas em Grupo Padrão (GP, n=5) para controle e cinco grupos experimentais com 20 animais cada, a saber: Grupo Eurocollins (GE), Grupo Soro Fisiológico 0,9% (GF), Grupo Glicose 50% (GG), Grupo Manitol 20% (GM), Grupo Salgadão -NaCl 7,5% (GS). Todos os animais dos grupos experimentos foram ainda distribuídos em quatro subgrupos de acordo com o seu tempo de coleta em: 0 h, 2 h, 6 h e 12 h. Foram avaliados a viabilidade celular a partir da reação com Methyl Thiazolyl Blue (MTT), além das dosagens de lactato e de alanino amino-transferase (ALT). Resultados -Em relação à dosagem de lactato, foi possível observar relativa melhora das soluções hipertônicas em comparação à eurocol...

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Section: Discussionsupporting

confidence: 74%

Uso de soluções hipertônicas para preservação hepática em ratos

Yamaki

Teixeira

Brito

2013

ABCD, arq. bras. cir. dig.

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“…The significant reduction found only in GI may be attributed to the group effect observed. In the present study it was not perceived the increase in serum activity of ALT, described by Siqueira et al 15 , probably for not achieving the magnitude of hypothermia they described. Despite the occurrence of group effect, alterations were not observed in ALP values, a fact similar to that described by Mello and Cordeiro 10 in dogs under venous anesthesia.…”

Section: Discussioncontrasting

confidence: 60%

Fluid heating system (SAF®): effects on clinical and biochemistry parameters in dogs submitted to inhalatory anesthesia

Atayde¹,

Franco²,

Silva³

et al. 2009

Acta Cir. Bras.

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PURPOSE: To evaluate and describe immediate effects of the infusion of saline solution heated by SAF® in bitches submitted to halothane anesthesia. METHODS: Thirteen bitches were employed and submitted to elective ovariohysterectomy in acclimatized operating room at 22ºC, allocated in two groups: GI, which received non-heated fluid and GII, which received fluid heated at 37ºC by SAF®. The following parameters were evaluated in 30-minutes intervals (M0, M30, M60 and M90): rectal and cutaneous temperatures (TR and TC), cardiac and respiratory frequencies (HR and ƒ), mean arterial blood pressure (MAP), serum concentration of urea, creatinin, serum activities of alanin aminotranspherasis (ALT), alkaline phosphatasis (ALP) and also hypnosis parameters. RESULTS: There were no significant alterations on clinical and biochemical, but there was group effect on mean arterial blood pressure, urea, ALT, ALP and hypnosis parameters. CONCLUSION: The isolated use of Fluid Heating System (SAF®) was not enough to avoid hypothermia or lead to significant clinical and biochemical alterations in bitches submitted to halothane anesthesia.

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Estudo funcional e morfológico da hipotermia sistêmica ou tópica no fígado de cães

Abstract: The selective hypothermia causes more lesions to the liver than the systemic.

Cited by 2 publications

References 8 publications

Uso de soluções hipertônicas para preservação hepática em ratos

Uso de soluções hipertônicas para preservação hepática em ratos

Fluid heating system (SAF®): effects on clinical and biochemistry parameters in dogs submitted to inhalatory anesthesia

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