Experimental model for fibroblast culture

Keira, Sidney Mamoru; Ferreira, Lydia Masako; Gragnani, Alfredo; Duarte, Ivone da Silva; Santos, Isabel A.

doi:10.1590/s0102-86502004000700004

Cited by 41 publications

(32 citation statements)

References 7 publications

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“…Primary human dermal fibroblasts (HDFs) were isolated through the outgrowth method described elsewhere [20] from redundant skin from cosmetic-plastic surgery (excision from the gluteal region of a seven year old female). The clearance to conduct this study was provided by UMMC ethical approval (PPUM/MDU/300/04/03, 22 April 2011).…”

Section: Methodsmentioning

confidence: 99%

A comparison study of different physical treatments on cartilage matrix derived porous scaffolds for tissue engineering applications

Moradi

Pramanik

Ataollahi

et al. 2014

Science and Technology of Advanced Materials

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Native cartilage matrix derived (CMD) scaffolds from various animal and human sources have drawn attention in cartilage tissue engineering due to the demonstrable presence of bioactive components. Different chemical and physical treatments have been employed to enhance the micro-architecture of CMD scaffolds. In this study we have assessed the typical effects of physical cross-linking methods, namely ultraviolet (UV) light, dehydrothermal (DHT) treatment, and combinations of them on bovine articular CMD porous scaffolds with three different matrix concentrations (5%, 15% and 30%) to assess the relative strengths of each treatment. Our findings suggest that UV and UV–DHT treatments on 15% CMD scaffolds can yield architecturally optimal scaffolds for cartilage tissue engineering.

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Section: Methodsmentioning

confidence: 99%

A comparison study of different physical treatments on cartilage matrix derived porous scaffolds for tissue engineering applications

Moradi

Pramanik

Ataollahi

et al. 2014

Science and Technology of Advanced Materials

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“…Primary keloid fibroblast culture was done by explant 19,20 by which the fractions corresponding to samples of adjacent skin and keloid were obtained. Fractions were placed in 15 ml conic tubes and exhaustively rinsed (six times) with 10 ml PBS (Phosphate-Buffered Saline, Cultilab, Brazil) containing penicillin (100Ul/ml, Gibco ® ) and streptomycin (100µ/ml, Gibco ® ) under vigorous agitation, changing tubes and PBS at each repetition.…”

Section: Obtainment Of the Fibroblast Culturesmentioning

confidence: 99%

In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts

et al. 2011

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Purpose:To quantify keloid fibroblasts after irradiation with 470nm blue LED, in vitro. Methods: Fibroblasts from keloid and adjacent skin have been obtained from 6 patients. Cells have been cultivated and maintained in DMEM culture medium. In Petri dishes, they were irradiated with energy doses of 6J, 12J and 18J. After 24 h, counting was done by the average of the triplicates for each sample. Results: There were no significant differences in the number of irradiated keloid fibroblasts at the studied doses (p=0.261). In adjacent skin fibroblasts, differences were observed (p=0.025) concerning the doses of 18 J and 6 J (p=0.03). Conclusions: There was a reduction in the number of adjacent skin fibroblasts irradiated with 470nm blue LED at the energy dose of 18 J compared to the ones irradiated at the energy dose of 6 J. There were no changes in keloid fibroblasts counting at any of the doses applied, 24 h after irradiation. Key words: Fibroblasts. Keloid. Phototherapy. Lasers. Skin. Laser Therapy, Low-Level. RESUMOObjetivo: Quantificar fibroblastos de quelóide após irradiação com LED azul de 470nm, in vitro. Métodos: Foram obtidos fibroblastos de quelóide e pele adjacente, de seis pacientes. As células foram cultivadas e mantidas em meio de cultura DMEM. Em placas de Petri, receberam irradiação com doses de energia de 6J, 12J e 18J. Após 24 horas a contagem foi feita pela média da triplicata para cada amostra. Resultados: Não houve diferença na quantidade de fibroblastos de quelóide irradiados nas doses estudadas (p=0,261). Observou-se diferença nos fibroblastos de pele adjacente (p=0,025), com relação às doses de 18 J e 6 J (p=0,03). Conclusões: Houve redução dos fibroblastos de pele adjacente irradiados com LED azul de 470 nm na dose de energia de 18 J em relação à dose de 6 J. Não houve alteração na quantidade de fibroblastos de quelóide nas doses aplicadas após 24 horas da irradiação.

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“…Recent technological advances in tissue engineering suggest its application in several medical fields, including periodontology (1). The cell culture technique allows the study of the biological behavior of cells, considering the kinetics of cell proliferation and the biosynthesis of many components of the extracellular matrix (2).…”

Section: Introductionmentioning

confidence: 99%

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

et al. 2013

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As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.

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Experimental model for fibroblast culture

Cited by 41 publications

References 7 publications

A comparison study of different physical treatments on cartilage matrix derived porous scaffolds for tissue engineering applications

A comparison study of different physical treatments on cartilage matrix derived porous scaffolds for tissue engineering applications

In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

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