Experimental model of cultured skin graft

Gragnani, Alfredo; Morgan, Jeffrey R.; Ferreira, Lydia Masako

doi:10.1590/s0102-86502004000700003

Cited by 4 publications

(5 citation statements)

References 11 publications

(8 reference statements)

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“…The Improving bio-molecular understanding and therefore accelerating the development of new therapies is also an advantage of this process. The disadvantage is that after a continuous period of growth, the cellular characteristics may change and become different to that of the donor including, and it has a lack of neuroendocrine signaling in relation to experimental animals 24,25 .…”

Section: Post-burn Time Intervalmentioning

confidence: 99%

Rat an experimental model for burns: A systematic review

Mitsunaga

Gragnani

Ramos³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To revise and systematize scientific knowledge of the experimental model for cutaneous burns in rats. METHODS:A bibliographical review from 2008 up to January 2011 in PubMed, EMBASE and LILACS was undertaken. Were used the keywords: animal models, burns and rats. 221 studies were identified, and 116 were selected. RESULTS:It was found that: 54/86 (62.7%) had third degree burns; 55/73 (75.3%) studied the back; 45/78 (57.6%) used heated water and 27/78 (35.9%) incandescent instruments; 39/78 (50%) studied systemic effects; 22/71 (31%) used ketamine associated with xylazine; 61/64 (95.3%) performed depilation with appropriate equipment; 36/72 (50%) used microscopy; more than 50% did not describe analgesia or antibiotics during the postoperative period; in 42/116 (36.2%) postoperative fluid therapy was performed; and the time interval after the burn, up to the beginning of the results analysis varied from 7s up to four weeks. Legislation issues on burn experiments are discussed. CONCLUSION:The hot water was the main method to induce burns those of third degree on the back, with anesthesia using ketamine and xylazine, after depilation. These were evaluated microscopically, without using analgesia or an antibiotic during the postoperative period. The studies were not very reproducible. CONCLUSÃO: Líquido aquecido foi o principal método para induzir queimadura de terceiro grau no dorso do animal, com anestesia usando quetamina e xilazina, após depilação, avaliados por microscopia, sem uso de analgesia ou antibióticos. Os estudos não são reprodutíveis.Descritores: Modelos Animais. Queimaduras. Pele. Revisão. Ratos.Mitsunaga Jr JK et al.

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Section: Post-burn Time Intervalmentioning

confidence: 99%

Rat an experimental model for burns: A systematic review

Mitsunaga

Gragnani

Ramos³

et al. 2012

Acta Cir. Bras.

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“…In order to measure accurately the penetration of LMWHA molecules and exclude other penetration possibilities through keratinocyte layers, a cultivation method must be designed properly that imitates what happens in nature and does not affect to a great extent the size of intercellular spaces and stratification process of keratinocytes. Common two‐phase cultivation technique that consists of cultivating epidermal cells on steel net with decellularized dermis is not applicable due to the absence of side walls on the grid and the possibility of these molecules to pass through the edges.…”

Section: Resultsmentioning

confidence: 99%

“…The Student's t test was used to compare results. Common two-phase cultivation technique that consists of cultivating epidermal cells on steel net with decellularized dermis 24 is not applicable due to the absence of side walls on the grid and the possibility of these molecules to pass through the edges.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive penetration of 5 nm hyaluronic acid molecules across the epidermal barrier (in vitro) and its interaction with human skin cells

Nashchekina

Raydan

2017

Skin Research and Technology

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Objective: Hyaluronic acid represents one of the major components of the extracellular environment. The main challenge remains in the ability to deliver these molecules noninvasively across the skin barrier, which can be overcome by the reduction in size to an extent that allows these molecules to pass across the skin barrier.The aim of this study was to measure the penetration and bioavailability of low molecular weight hyaluronic acid to cross an epidermal barrier model. Methods:Determining the quantity of hyaluronic acid in the test solutions was carried with method of photocolorimetry analysis. Investigation of the interaction of cells with LMWHA was studied with a confocal microscope. Results:The study showed that LMWHA is able to cross the epidermis. Most effective penetration level is during the first 6 hours reaching 75%, and then the concentration started to decline and reached the equilibrium state within the following 2 hours.Confocal laser microscopy demonstrated different distribution and behavior of these molecules among the keratinocytes and fibroblasts. Conclusion:Reducing the size of hyaluronic acid to 5 nm enhance their transport across the epidermal layer. The concentration of hyaluronic acid molecules was higher on the fibroblast surface in comparison to their extracellular environment. K E Y W O R D Sfibroblasts, keratinocytes, low molecular weight hyaluronic acid (LMWHA), skin barrier

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“…Ever since, several authors have proposed several techniques of keratinocytes isolation in order to optimize the established protocols of cultivation [17][18][19][20] . The methodology of human keratinocytes culture employed in this present work was revised and appropriated by Gragnani, Morgan and Ferreira in our laboratory 6,7 .…”

Section: Discussionmentioning

confidence: 99%

“…The culture protocol defined by Green, Kehinde, Thomas 4 and Green 5 , was applied in the Laboratory of Cell Culture UNIFESP's Plastic Surgery Division by Gragnani, Morgan, Ferreira 6,7 .…”

Section: Culture Of Primary Human Keratinocytesmentioning

confidence: 99%

Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress

et al. 2010

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PURPOSE: To evaluate effects of oxidative stress and supplementation of keratinocyte growth factor (KGF) on cultivated human keratinocytes. METHODS: Oxidative stress was produced through addition of hydrogen peroxide (H2O2) to the culture medium. Cultivated human keratinocytes were divided in 4 groups: Group control (G C), Group KGF (G KGF), Group H2O2 (G H2O2), Group H2O2 and KGF (G H2O2-KGF). Each experiment was accomplished with the same lineage cultivated keratinocytes, in triplicate. Cell viability was evaluated by trypan blue exclusion assay. RESULTS: The results showed that the culture medium supplemented with KGF presented a small rate of cell viability when compared to cells only in culture medium (p<0,001). It demonstrated that only the growth factor does not have protector effects for cells in vitro. However, in front of the oxidative stress produced by addition of hydrogen peroxide to the medium, KGF showed a beneficial effect, protecting cells when compared to the group that suffered hydrogen peroxide action but had not been exposed to KGF (p<0,001). CONCLUSION: KGF determined protection to the primary human keratinocytes exposed to oxidative stress.

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Experimental model of cultured skin graft

Cited by 4 publications

References 11 publications

Rat an experimental model for burns: A systematic review

Rat an experimental model for burns: A systematic review

Noninvasive penetration of 5 nm hyaluronic acid molecules across the epidermal barrier (in vitro) and its interaction with human skin cells

Keratinocyte growth factor protected cultured human keratinocytes exposed to oxidative stress

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