Uso de animais em cirurgia experimental

Schanaider, Alberto; Silva, Paulo César

doi:10.1590/s0102-86502004000400014

Cited by 57 publications

(56 citation statements)

References 14 publications

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“…Despite the existence of other experimental animal models for SLNB in the stomach, in dogs 33 , pigs 32 and rabbits 34 , the model presented in this study had the following main advantages: low purchase cost of the animals, ease of handling, absence of the need for preoperative fasting, high animal replacement capacity (especially due to the short reproductive cycle), good adaptation to the laboratory environment, and limited humanistic sentimental appeal 30,31,35 (Chart 1). Moreover, it was the first national attempt that used rabbits [32][33][34] , presented an acceptable mortality rate and practicality in the execution of the exclusively intramuscular anesthesia, without the need for definitive intubation 35 .…”

Section: Discussionmentioning

confidence: 99%

“…Fundamental, following the studies of Claude Bernard (1865), the Nuremberg Cod (1947) and the Helsinki Declaration (1975), it is advisable that experiments on human beings only be carried out based on evidence from research on animals [29][30][31] .…”

Section: Introductionmentioning

confidence: 99%

“…However, they have limitations, such as their small sample size 9,11 , high cost, and large sentimental humanistic appeal, especially when dogs are involved. Also, for the establishment of an experimental model, it is necessary to take into consideration the characteristics of the animal and its similarities to humans [29][30][31]35 .…”

Section: Introductionmentioning

confidence: 99%

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Animal model for training in sentinel lymph node biopsy of the stomach through combined methods

Alves¹,

Lopes

Meirelles³

et al. 2012

Acta Cir. Bras.

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PURPOSE:Create and validate a proposed animal model for training in sentinel lymph node biopsy of the stomach. METHODS: In thirty-two rabbits, through a laparotomy, they received a subserosal injection of 0.1 ml of phytate labeled with technetium-99m (0.2 mCi) in the anterior wall of the gastric corpus, followed by 0.2 ml of Blue Patent V ® 2.5%, through the same puncture site. Suspicious lymph nodes were searched in vivo at five, ten and 20 minutes, both visually (Blue Patent stained lymph nodes) and with a manual gamma radiation detector (to detect suspected radioactive lymph nodes). After 20 minutes, was performed resection of these for further evaluation of radioactivity (ex vivo) and histological study. RESULTS: Lymph nodes were identified in 30 rabbits (Average of 2.2 lymph nodes per animal). Of the 90 suspected lymph nodes that occurred in the study, 70 cases (77.8%) were histologically confirmed for lymphoid tissue. Of these, the majority were located in the periesophageal region of the gastric fundus. The sample presented a mortality rate of 6.25% and nine complications related to the method, which interfered in the identification of the lymph nodes. CONCLUSION: The animal model for sentinel node biopsy in rabbit stomachs proved to be feasible, with low complexity and reproduced the difficulties encountered for gastric lymph node biopsy in humans, being adequate for surgical training. Key words: Models, Animal. Sentinel Lymph Node Biopsy. Stomach. Coloring Agents. Technetium. Rabbits. RESUMO OBJETIVO:Criar e validar uma proposta de modelo animal para o treinamento em pesquisa de linfonodos sentinelas no estômago. MÉTODOS: Em trinta e dois coelhos, mediante laparotomia, foi injetado na subserosa da parede anterior do corpo gástrico, 0,1 ml de fitato marcado com tecnécio-99m (0,2 mCi), seguido pelo mesmo orifício, de 0,2 ml de Azul Patente V ® 2,5%. A cavidade abdominal foi avaliada, in vivo, por meio de inspeção para pesquisa de suspeitas de linfonodos azuis e com detector manual de radiação gamma aos cinco, dez e 20 minutos para pesquisa de suspeitas de linfonodos radioativas. Após 20 minutos, foi realizada a ressecção dessas para posterior avaliação da radioactividade (ex vivo) e estudo histológico. RESULTADOS: Foram identificados linfonodos em 30 coelhos (Média de 2,2 linfonodos por animal). Das 90 suspeitas de linfonodos ocorridas no estudo, em 70 casos (77,8%) obteve-se confirmação histológica para tecido linfóide. Destas, a maioria estava localizada na região periesofágica do fundo gástrico. A amostra apresentou taxa de mortalidade de 6,25% e nove intercorrências relacionadas ao método, que interferiram na identificação dos linfonodos. CONCLUSÃO: O modelo animal para pesquisa de linfonodos sentinelas em estômago de coelhos mostrou-se factível, pouco complexo e reproduziu as dificuldades encontradas para a pesquisa de linfonodos gástricos em humanos, sendo adequado para o treinamento cirúrgico. Descritores: Modelos Animais. Biópsia de Linfonodo Sentinela. Estômago. Corantes. Tecnécio. Coelh...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Animal model for training in sentinel lymph node biopsy of the stomach through combined methods

Alves¹,

Lopes

Meirelles³

et al. 2012

Acta Cir. Bras.

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“…In this research, as it was an experimental study on live animals, it was essential to have knowledge of all the procedures that are aimed at the well-being and safety of the animal and of the researcher [17][18][19][20] . Therefore, all norms were followed regarding ethical issues related to the proper and responsible handling of the animals.…”

Section: Correlation Between the Inflammatory Intensity And Size Of Tmentioning

confidence: 99%

Effect of the period of extrinsic mechanical compression following sclerotherapy in veins in rabbit ears

Ivo¹,

Ivo

Salles

et al. 2011

Acta Cir. Bras.

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Purpose:Research whether a post-sclerotherapy venous compression period of up to 120 hours is sufficient to avoid reperfusion in treated veins; whether there is a relationship between the inflammatory intensity in venous walls and adjacent tissue and the size of venous thrombosis; whether the intensity of the post-sclerotherapy inflammation varies with the period of compression; whether there is a relationship between the presence of hemosiderin in the tissues adjacent to the sclerosing blood vessels and venous blood clots. Methods: Twenty eight rabbits, all male, were utilized, distributed into four groups (0, 24, 72 and 120). All the animals were administered with 0.25 ml of 1% polidoconal solution and, as a control, 0.25 ml of 0.9% sodium chloride solution in the marginal dorsal vein of the right and left ears, respectively. Mechanical compression was applied to the perfused stretch of the vein, except for the animals in group 0. The period of compression varied from 0 to 120 hours in the groups. An anatomopathological examination of the section of the right and left marginal dorsal veins of all the animals was conducted. Results: There was no significant difference among the various compression periods, both in terms of the degree of vein thrombosis and in the inflammatory intensity in both ears of the various groups. A positive and significant correlation was observed between the inflammatory intensity and the size of the thrombus and in the occurrence of thrombi and hemosiderin. Conclusions: A compression period of up to 120 hours is not sufficient to prevent reperfusion in sclerosing blood vessels. The intensity of tissue inflammation is related to the size of the thrombus, but not to the compression period. The presence of hemosiderin in the tissues adjacent to the vessels subjected to sclerosis is related to the presence of venous coagulation. Key words: Sclerotherapy. Sclerosing Solutions. Varicose Veins. Animal Experimentation. Academic Dissertations. Rabbits. RESUMOObjetivo: Pesquisar se o tempo de compressão venosa de até 120 horas pós-escleroterapia é suficiente para evitar reperfusão nas veias tratadas; se há relação entre a intensidade inflamatória na parede venosa e tecidos adjacentes e o tamanho do trombo venoso; se a intensidade da inflamação pós-escleroterapia varia com o tempo de compressão; se há relação entre a presença de hemossiderina nos tecidos adjacentes ao vaso esclerosado e coágulo venoso. Métodos: Utilizaram-se 28 coelhos, machos, distribuídos em quatro grupos (0, 24, 72 e 120). Em todos os animais foram administrados 0,25 ml de solução de polidocanol 1% e, como controle, 0,25 ml de solução de cloreto de sódio 0,9% na veia marginal dorsal das orelhas direita e esquerda, respectivamente. Realizou-se compressão mecânica em trecho da veia perfundida, exceto nos animais do grupo 0. O tempo de compressão variou de 0 a 120 horas nos grupos. Realizou-se exame anatomopatológico de trecho das veias marginais dorsais direita e esquerda de todos os animais. Resultados: Não houve dife...

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“…Em caso de morte do animal, a pena é aumentada de um sexto a um terço. Esta lei adota parcialmente as noções contidas nos três "R" 11 e, mesmo que de forma inadequada, é a única vigente no País que pode ser aplicada à prática da experimentação animal 25 5,29 .…”

Section: Legislação Brasileiraunclassified

Experimentação animal: ética e legislação brasileira

2008

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Este artigo trata da experimentação animal sob os pontos de vista da ética e da política, fazendo uma abordagem histórica do tema no cenário legislativo brasileiro. Os animais de laboratório desempenharam um papel decisivo para o desenvolvimento da ciência e continuam tendo um papel essencial na pesquisa biomédica, no entanto, sua utilização sempre despertou manifestação de diferentes naturezas. Apesar de as primeiras incursões na pesquisa experimental terem sido iniciadas há, pelo menos, quatro séculos, é incipiente o estabelecimento de normas que regulamentem a utilização de animais na América Latina. No Brasil, embora não haja uma lei específica em relação a essa utilização, algumas iniciativas já foram tomadas. As discussões sobre este tema continuam ocorrendo, contudo, a demora para definir uma lei que regulamente o uso de animais em pesquisa tem dificultado a padronização de procedimentos necessários à sistematização dos conhecimentos desenvolvidos no País. Termos de indexação: Ética. Experimentação animal. Legislação. A B S T R A C T This article covers animal experimentation under the viewpoint of ethics and politics, and does a historical review of the theme in the Brazilian legislative scenario. Laboratory animals play a decisive role in the development

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Uso de animais em cirurgia experimental

Cited by 57 publications

References 14 publications

Animal model for training in sentinel lymph node biopsy of the stomach through combined methods

Animal model for training in sentinel lymph node biopsy of the stomach through combined methods

Effect of the period of extrinsic mechanical compression following sclerotherapy in veins in rabbit ears

Experimentação animal: ética e legislação brasileira

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