Ligation of the abdominal esophagus decreases scorpion toxin-induced gastric secretion in rats

Abstract

PURPOSE: Scorpion toxin purified from Tityus serrulatus venom (Tx) induces an increase in volume, acidity and pepsin secretion in the gastric juice of rats. Ligation of oesophagus has been shown to reduce the acid gastric secretion in rats. The aim of this paper was to determine the influence of the esophageal ligation on gastric secretion induced by Tx in rats METHODS: Forty-four male albino rats were given water ad libitum, but no food for 20 to 24 hours, anesthetized with urethane and the trachea and jugular vein cannulated. Cervical or abdominal esophageal ligation or sham-operations were performed before and after the injection of 0.25 mg/kg of scorpion toxin (fraction T1) into the jugular vein. One hour later, the volume, acidity, pH and peptic activity of gastric juice were determined. RESULTS: The scorpion toxin induced an increase in gastric juice volume, acidity and pepsin output and a decrease in pH when injected into the vein of intact animals or in sham-operated animals. Cervical esophagus ligation did not interfere with the effects of toxin, however, ligation of the abdominal esophageal decreased the toxin effect on the rat stomach. CONCLUSION: Ligation of the abdominal esophagus decreases the gastric secretion induced by scorpion toxin.Key words : Tityus serrulatus. Scorpion venoms. Gastric secretion. Esophageal ligation. 167RESUMO OBJETIVO: A toxina de escorpião purificada do veneno do escorpião Tityus serrulatus (Tx) induz um aumento no volume, acidez e secreção de pepsina no suco gástrico de ratos. A ligadura do esôfago diminui a secreção ácida do estômago em ratos. O objetivo deste trabalho foi determinar a influência da ligadura do esôfago sobre a secreção gástrica induzida pela Tx em ratos. MÉTODOS: 44 ratos machos, brancos foram administrados água ad libitum, mas não alimentados por 20 a 24 horas, anestesiados com uretana e canulados a traquéia e a veia jugular. Foram realizadas as ligaduras do esôfago cervical ou abdominal ou operações simuladas antes e após a administração na veia jugular de 0,25 mg/kg de toxina de escorpião (fração T1). Uma hora após foram determinados o volume, acidez, pH e atividade péptica do suco gástrico. RESULTADOS: A toxina de escorpião induziu um aumento do volume do suco gástrico, da acidez gástrica e da produção de pepsina e uma diminuição do pH quando injetada na veia de animais não operados ou com operação simulada. A ligadura do esôfago cervical não interferiu nos efeitos da toxina, enquanto a ligadura do esôfago abdominal diminuiu os efeitos da toxina no estômago do rato. CONCLUSÃO: A ligadura do esôfago abdominal diminui a secreção gástrica estimulada pela toxina de escorpião.Descritores: Tityus serrulatus. Veneno de escorpião. Secreção gástrica. Ligadura do esôfago.

The fractal nature of human gastro‐oesophageal reflux

“…Our hypothesis could be tested by measuring the effect on gastric acidity of perfusing the distal oesophagus with hydrochloric acid. As regards, in vivo ligation of the abdominal oesophagus in rats has been reported to decrease gastric acidity 17 . Conceivably, GERD subjects might have an impaired oesophageal‐gastric feedback mechanism that results in increased gastric acid that can reflux from the stomach into the oesophagus.…”

Continuous distal oesophageal acidification decreases postprandial gastric acidity in healthy human subjects

“…Interestingly, one subject in the present study spontaneously reported increased secretion of saliva with oesophageal HCl infusion, but not with gastric HCl infusion. Finally, in vivo ligation of the abdominal oesophagus in rats has been reported to decrease gastric acidity 26 …”