Estudo comparativo das reações teciduais ao implante de pericárdio bovino e a inclusão de politetrafluoroetileno expandido em ratos

Filho, Douglas Haddad; Marques, António; Kafejian-Haddad, Andréa Paula; Zveibel, Deborah K.

doi:10.1590/s0102-86502004000200009

Cited by 2 publications

(4 citation statements)

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“…However, among the 7 and 30 day subgroups, the number of lymphocytes decreased, concurring with the findings of Kafejian et al 9 and Haddad-Filho et al 18 , noted an increase in lymphocytes during the periods, which is contrary to what was observed in the present study, nevertheless, confirming the chronicity of the acute inflammatory reaction. The presence of macrophages also confirms the chronicity of the inflammatory reaction.…”

Section: Discussionsupporting

confidence: 91%

“…Nevertheless, upon isolating this data, and using the mean, we observed a real trend that is the reduction of the number of neutrophils, corresponding to resolution of the inflammatory process. Even though limited to 30 days evolution, the results of Kafejian et al 9 and Haddad-Filho et al 18 , the latter, in a comparative study between e-ptfe and bovine pericardium, confirmed the results obtained. When the silicone, bonded e-ptfe and control groups were compared, in the 7 days subgroup, a significant increase was noted in the number of neutrophils in the two-implant groups compared to the control group.…”

Section: Discussionsupporting

confidence: 64%

“…This data demonstrates resolution of the inflammatory-repair process and interference of materials on it. Haddad-Filho et al 18 observed similar results during the 30 day period. Regarding the e-ptfe, Kafejian et al 9 observed an increase in capillaries at the end of 30 days.…”

Section: Discussionmentioning

confidence: 56%

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Comparison between textured silicone implants and those bonded with expanded polytetrafluoroethylene in rats

Filho

Zveibel

Alonso³

et al. 2007

Acta Cir. Bras.

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Comparison between textured silicone implants and those bonded with expanded polytetrafluoroethylene in ratsComparison between textured silicone implants and those bonded with expanded polytetrafluoroethylene in rats ABSTRACT Purpose:Comparison of the inflammatory reaction promoted by textured silicone implants and that caused by the implant bonded with e-ptfe. Methods: One-hundred and fifty rats were divided into three equal groups (control, silicone, and bonded e-ptfe). These groups were subdivided into five groups, according to the second operation, i.e., 7,30,60,90 and 180 days. Histology of the peri-implant tissue was analyzed by morphometry with blood count (neutrophilos, lymphocytes, macrophages, fibroblasts and capillaries). Results: Comparison of subgroups 7,30,60,90, 180 days: -neutrophils: silicone: > in subgroup 7 days; bonded e-ptfe: > in subgroups 7 and 30 days; -lymphocytes: silicone: > in subgroup 7 and 180 days; bonded e-ptfe: > in subgroup 180 days; -macrophages: silicone: > in subgroup 7 and 60 days; bonded e-ptfe: > in subgroup 7,30 and 60 days; -fibroblasts: silicone: > in subgroup 30 and 60 days;-vascular volume: silicone: in subgroup 7, 60 and 90 days; bonded e-ptfe: > in subgroup 7 days. Comparison of groups: neutrophils : 7 days: > in silicone and bonded e-ptfe; 30 days: > in bonded e-ptfe; -lymphocytes:-7,30,90 and 180 days: in the control; macrophages:-7,30 and 60 days: > in silicone & bonded e-ptfe; 180 days > in silicone; fibroblasts: -7,30 and 90 days: > in silicone and bonded e-ptfe; 180 days: > in bonded e-ptfe; vascular volume -7,60,90 and 180 days: > in silicone and bonded e-ptfe; 30 days: > in bonded e-ptfe. Conclusions: The acute stage of the inflammatory response was more severe and irregular in the silicone implant; both the silicone implant and the silicone bonded with e-ptfe promoted chronic inflammatory reaction and weak foreign body inflammatory response. These reactions were greater in the silicone implant group. Key words: Silicone Elastomers. Silicone Gels. Polytetrafluoroethylene. Rats, Wistar. RESUMO Objetivo:Comparar a reação inflamatória provocada pelo implante de silicone texturizado, com aquela causada por este recoberto com PTFE-E. Métodos: Foram utilizadas 150 ratas, divididos em três grupos igruais (controle, silicone e recoberto PTFE-E). Os grupos foram subdivididos em cinco subgrupos, ou seja, 7, 30, 60, 90 e 180 dias, de acordo com a data do segundo ato operatório. O tecido perimplante foi analisado histologicamente, por meio de técnica morfométrica, com contagem de neutrófilos, linfócitos, macrófagos, fibroblastos e capilares. Resultados: Comparação dos subgrupos 7, 30, 60, 90 180 dias: -neutrófilos -silicone: > no subgrupo 7 dias; rec-ptfe: > nos subgrupos 7 e 30 dias; -linfócitos: silicone: > no subgrupo 7 e 180 dias; rec-ptfe: > no subgrupo 180 dias; -macrófagos: silicone: > no subgrupo 7 e 60 dias; rec-ptfe: > no subgrupo 7, 30 e 60 dias; -fibroblastos: silicone: > no subgrupo 30 e 60 dias; -volume vascular: silicone: > no subrupo 7, 60 e 90 dias; re...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 64%

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Comparison between textured silicone implants and those bonded with expanded polytetrafluoroethylene in rats

Filho

Zveibel

Alonso³

et al. 2007

Acta Cir. Bras.

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“…Em seguida, foi realizada uma incisão de 1,5 cm, sentido dorso-ventral na pele da região abdominal esquerda. Retirou-se, de cada camundongo, um fragmento muscular que abrangia o músculo oblíquo externo, músculo oblíquo interno e o músculo transverso abdominal e no mesmo local foi implantado um fragmento de pericárdio tratado de 1 cm 2 [9].…”

Section: Delineamento Experimentalunclassified

Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

Costa

Silva²,

Andrade

2016

Acta Scientiae. Vet.

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Background: Lately, the use of biological materials has been widely indicated in surgical procedures to restore structure and function of injured tissues. Bioimplants require different conservation techniques; among these, glutaraldehyde preservation stands out owing to its higher antimicrobial efficiency as compared to glycerin. In view of the need to determine a concentration of glutaraldehyde that can act as a biocide but do not cause undesirable tissue reactions, this study aimed to identify and quantify gross and microscopic tissue alterations after implantation of bovine pericardium, which was preserved in various concentrations of glutaraldehyde, in the abdominal wall of mice.Materials, Methods & Results: Fresh pericardia from 18 bovines were fractioned into 1cm2 samples and treated with a 98% glycerin solution for 30 days (control group), or 0.625%, 1%, and 1.5% glutaraldehyde solution for 18 days (experimental groups). An abdominal muscle fragment was excised from each mouse, and a 1-cm2 fragment of preserved pericardium was implanted in the area. Sixty mice (n = 15 per treatment) divided into groups were observed for 7, 14, and 30 days, and five animals from each group were euthanized at each time point for gross and microscopic examination. Fragments of the implants and adjacent skin lesions were harvested, fixed in formalin, and processed for routine histology and microscopic analysis. Both the type of inflammatory infiltrate and the repair process of the tissue response were similar between the groups that received glycerin-preserved pericardium and those that were subjected to pericardium preserved with 0.625% glutaraldehyde. Animals that received 1% glutaraldehyde-preserved implants and were examined 30 days thereafter exhibited a chronic, intense reaction with fibrosis and necrosis of the abdominal wall muscles, as well as calcification and presence of giant cells, when compared to the animals examined at 7 and 14 days in the same treatment group. These changes were also present and more intense in animals that received 1.5% glutaraldehyde-preserved pericardium examined at 14 and 30 days later, with tissue destruction and impaired incorporation of the implant into the adjacent muscle tissue.Discussion: The continuous cell destruction observed in animals treated with implants preserved with 1% or 1.5% glutaraldehyde is a hallmark of chronic inflammation, since several inflammatory cell molecules contribute to this lesion. A cycle is created: continuous degradation sustains inflammation, and inflammatory molecules contribute to the process of cell destruction. Consequently, we conclude that the use of glutaraldehyde at concentrations of 1 or 1.5% is not feasible for preservation of biological materials. Tissue repair was chronologically more effective in the group treated with glycerinpreserved implants, since animals treated with glutaraldehyde-preserved implants needed a longer period to restore due to presence of a persistent inflammatory response, immunogenicity, calcification, and deficient remodeling. The ideal preservative for biological materials should not cause chronic and/or intense inflammatory reaction in order to preserve the implant’s structure and allow its perfect incorporation into the tissue, even if the chosen preservative is flexible and exhibits disinfectant properties. Therefore, we conclude that glutaraldehyde at concentrations of 0.625% to 1% is suitable as a preservative for biomaterials because the tissue reaction it causes is tolerable; additionally, glutaraldehyde at concentrations close to 1% has been described to have sterilizing properties.

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Estudo comparativo das reações teciduais ao implante de pericárdio bovino e a inclusão de politetrafluoroetileno expandido em ratos

Cited by 2 publications

References 6 publications

Comparison between textured silicone implants and those bonded with expanded polytetrafluoroethylene in rats

Comparison between textured silicone implants and those bonded with expanded polytetrafluoroethylene in rats

Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

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