Efeitos da castração pós-natal sobre o tecido pulmonar após sepse experimental de origem abdominal em ratos

d'Acâmpora, Armando José; Bernhardt, Joel Antonio; Serafim, João Daniel May; Farias, Débora Cadore de; Tramonte, Ricardo

doi:10.1590/s0102-86502004000200006

Cited by 3 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the more outstanding changes include the marked vascular congestion, necrosis, and infiltration of mononuclear cells in agreement with previous studies [22] in lung tissue.…”

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Increased NTPDase Activity in Lymphocytes during Experimental Sepsis

Bertoncheli

Zimmermann

Jaques

et al. 2012

The Scientific World Journal

View full text Add to dashboard Cite

We investigated in rats induced to sepsis the activity of ectonucleoside triphosphate diphosphohydrolase (NTPDase; CD39; E.C. 3.6.1.5), an enzyme involved in the modulation of immune responses. After 12 hours of surgery, lymphocytes were isolated from blood and NTPDase activity was determined. It was also performed the histology of kidney, liver, and lung. The results demonstrated an increase in the hydrolysis of adenosine-5′-triphosphate (ATP) (P < 0.01), but no changes regarding adenosine-5′-monophosphate (ADP) hydrolysis (P > 0.05). Histological analysis showed several morphological changes in the septic group, such as vascular congestion, necrosis, and infiltration of mononuclear cells. It is known that the intracellular milieu contains much more ATP nucleotides than the extracellular. In this context, the increased ATPasic activity was probably induced as a dynamic response to clean up the elevated ATP levels resulting from cellular death.

show abstract

“…Among the more outstanding changes include the marked vascular congestion, necrosis, and infiltration of mononuclear cells in agreement with previous studies [22] in lung tissue.…”

Section: Discussionsupporting

confidence: 91%

“…In this study, histological analysis showed several morphological changes in organs analyzed from septic group in tissues such as kidney, liver, and lung. Among the more outstanding changes include the marked vascular congestion, necrosis, and infiltration of mononuclear cells in agreement with previous studies [ 22 ] in lung tissue.…”

Section: Discussionsupporting

confidence: 91%

Increased NTPDase Activity in Lymphocytes during Experimental Sepsis

Bertoncheli

Zimmermann

Jaques

et al. 2012

The Scientific World Journal

View full text Add to dashboard Cite

show abstract

“…The sepsis is polymicrobial and simulates the perforated appendicitis or diverticulitis 26 . It is believed that this experimental model is an appropriate study method to evaluate and to control the septic phenomena from its installation to the moment of failure of the organs and systems in different times in this process 28 . The experimental design of this study was elaborated in a such way that the evidences of the antiinflammatory effect of the sinvastatin in the abdominal sepse were evaluated in currently used biological models.…”

Section: Discussionmentioning

confidence: 99%

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

et al. 2006

View full text Add to dashboard Cite

Purpose: Statins are widely recognized as hypolipemic drugs, but some studies have observed anti-inflammatory and immunomodulatory effects, known as pleiotropic. The aims of this work was to study possible anti-inflammatory effects of simvastatin in abdominal sepsis. Serum pro-inflammatory cytokines and leukocytes count were determined in an experimental model of abdominal sepsis, using cecal ligation and puncture (CLP) in rats. Methods: Twenty eigth Wistar rats weighing 285±12g were randomly divided in: CLP/Sinvastatin rats (n=7), treated with 10 mg/Kg of oral simvastatin 18 and 2 hs berofe CLP; CLP/Saline group rats (n=7), treated with oral saline; group Sham/Simvastatin (n=7), treated with simvastatin, and group Sham/Saline (n=7), treated with saline. Serum TNF-α, IL-1β and IL-6 by ELISA and total leukocytes, neutrophils, lymphocytes, and eosinophils were determined 24 hs after CLP. ANOVA and Tukey test were used considering significant p<0.05. Results: It was demonstrated that serum TNF-α, IL-1β and IL-6 were respectively 364,8±42pg/mL; 46,3±18pg/mL and 28,4±13pg/mL in CLP/Sinvastatin rats, significantly lower (p<0.05) than in group CLP/Saline (778,5±86pg/ml; 176,9±46pg/ ml; 133,6±21 pg/ml, respectively). The same results were observed in total leukocytes and neutrophils counts. Conclusion: These results clearly demonstrate that simvastatin is an effective agent that reduces cytokines levels and leukocyte count in sepsis, independently of its well-known lipid-lowering effects. Thus, HMG-CoA reductase inhibitors like simvastatin have important anti-inflammatory effects in abdominal sepsis in rats. Key words: Statin. Inflammation. Abdominal sepsis. Wistar rat. Cytokine. Leukocyte. RESUMO Objetivo:As estatinas são agentes reconhecidamente hipolipemiantes. Vários estudos têm revelado que eles têm ações pleiotrópicas, como antiinflamatória e imunomoduladora. Tentando-se entender o papel antiinflamatório da sinvastatina na sepse, foram analisados os níveis de citocinas pró-inflamatórias e contagem de leucócitos em modelo de sepse abdominal por ligadura e punção do ceco (LPC) em ratos. Métodos: Foram utilizados 28 ratos Wistar pesando 285±12g, assim divididos: grupo sepse (n=14), submetidos a LPC e grupo sham (n=14), submetidos a laparotomia e manipulação suave do ceco. No grupo LPC/sinvastatina (n=7) os ratos receberam 10mg/kg de sinvastatina via oral 18 e 2 horas antes da LPC e no grupo LPC/salina (n=7) os ratos receberam injeção oral de solução salina 0,9 %. Os animais dos grupos sham/sinvastatina (n=7) e sham/salina (n=7) receberam o mesmo tratamento. Dosagem de TNF-α, IL-1β e IL-6 por ELISA e contagem de leucócitos totais, neutrófilos, linfócitos e eosinófilos foram realizadas em todos os animais. Análise estatística foi feita pelo ANOVA e teste de Tukey, com significância p<0,05. Resultados: Ficou demonstrado que as dosagens de TNF-α, IL-1β e IL-6 atingiram valores de 364,8±42pg/ml; 46,3±18pg/ml e 28,4±13pg/ml no grupo submetido à sepse e tratados com sinvastatina, significantemente mais baixos do que...

show abstract

Median lethal needle caliber in two models of experimental sepsis

d'Acâmpora

Locks

2014

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE:To estimate the median lethal needle caliber (LC50) of a new experimental sepsis model and compare it to the LC50 of the cecal ligation and puncture (CLP) sepsis model. METHODS:Male albino Wistar rats were studied (n=22). Animals were allocated into two study groups. In Group I, experimental sepsis was induced by cecal ligation and puncture. In Group II, experimental sepsis was induced by ascending colon ligation and cecal puncture. Up-and-down method was used to determinate the LC50. RESULTS: LC50 in

show abstract

Efeitos da castração pós-natal sobre o tecido pulmonar após sepse experimental de origem abdominal em ratos

Cited by 3 publications

References 22 publications

Increased NTPDase Activity in Lymphocytes during Experimental Sepsis

Increased NTPDase Activity in Lymphocytes during Experimental Sepsis

Effects of simvastatin in abdominal sepsis in rats<A NAME="volta1"></A>

Median lethal needle caliber in two models of experimental sepsis

Contact Info

Product

Resources

About