Experimental model of double wounds on the rats back , in order to study the skin cicatrization process on rat treated with cellulose coat

Cabral, Lecy Marcondes; Ferreira, Lydia Masako; Simões, Manuel de Jesus; Mora, Ofelia

doi:10.1590/s0102-86502003001100010

Cited by 4 publications

(2 citation statements)

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“…For surgery, rats were anesthetized intramuscularly in the gluteal region of the right limb with xylazine (10 mg/kg) and tiletamine/zolazepam (30 mg/kg) [ 23 – 26 ]. For in vivo assay, surgical excision of 2 cm in diameter was made in dorsal area [ 25 , 27 ]. For the experiments, 4 rats were tested at 4, 8, 15, and 21 days.…”

Section: Methodsmentioning

confidence: 99%

Natural Film Based on Pectin and Allantoin for Wound Healing: Obtaining, Characterization, and Rat Model

Valle

Acuña

Ríos-Arana

et al. 2020

BioMed Research International

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Introduction. In our days, several approaches reported the use of natural compounds in medical applications. Among them, pectin and allantoin are nontoxic, biocompatible, and biodegradable; however, its use for possible wound healing therapeutics is still limited. Pectin and allantoin have been applied in pharmaceutical industry and beauty cosmetic and could be also applied as scaffolds for tissue regeneration, wound healing, and so on. The aim of this study was to combine by the first time two natural ingredients to develop a new biomaterial to treat skin injuries in a rat model. Methods. For the hydrogel development, new synthesis parameters were established for the obtaining of the film such as temperature, mixing velocity and time, and drying temperatures as well. To enrich the film, the allantoin concentrations were set at 90 wt% and 100 wt% of pectin used. By in vivo assay, films were tested in wound healing in female Wistar rats, 190 ± 10 g in weight and 2 months aged. Results. The obtained films comprise 2 well-differentiated layers, one layer rich in allantoin, which will be the regenerative layer, and one rich in pectin, which will work as an antimicrobial and protective layer to the wound. These were characterized by swelling kinetics, Fourier transform of the infrared spectrum of absorption (FTIR) spectroscopy, and contact angle. The morphology and topography were determined by scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). In vivo assay showed remarkable reduce in a time period in a wound healing process when the film was used. The results show that the use of PA (Pectin-Allantoin) hydrogel reduces the total healing time by 25% approximately. Conclusions. Pectin-Allantoin (PA) film has potential use in medical applications as wound healing material promoting healthy tissue renewal.

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Section: Methodsmentioning

confidence: 99%

Natural Film Based on Pectin and Allantoin for Wound Healing: Obtaining, Characterization, and Rat Model

Valle

Acuña

Ríos-Arana

et al. 2020

BioMed Research International

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“…Os procedimentos cirúrgicos foram realizados de acordo com as normas para a prática didático-científica da vivissecção de animais (lei 6638/08 -05/1979) com os princípios éticos da experimentação animal (COBEA, 1991) em condições de assepsia e anestesia geral, seguindo metodologia proposta por Cabral (2003).…”

Section: Procedimento Cirúrgico Para a Realização Dos Experimentos Deunclassified

Avaliação e caracterização proteica do muco de Phyllocaulis boraceiensis sobre a capacidade proliferativa de fibroblastos, células endoteliais e em modelos de cicatrização.

Piza¹

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Biocompatibility and cutaneous reactivity of cellulosic polysaccharide film in induced skin wounds in rats

Lucena

Júnior

Lira

et al. 2015

J Mater Sci: Mater Med

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The development of a skin substitute suitable for immediately performing the function of the lost dermis and epidermis could result in a positive impact on the treatment of patients with extensive skin lesions. A biopolymer film was applied to skin wounds to investigate the biocompatibility and cutaneous reaction and to test its activity as a mechanical barrier and conductor in the healing process. Forty Wistar rats of both sexes were used in the present study. Two excisions were performed in the dorsal part of the skin flaps. The polysaccharide film was applied over one of the incisions and other incision was washed with saline. The time spent for complete healing of both lesions was virtually the same in both groups, during 21 days of observation. The film remained attached to the bed of the exposed wound for an average period of 6 days. There were no statistically significant differences with regard to lesion measurement area at assessment times of 2nd, 7th and 14th postoperative days. At day 21, the scar area showed a significant difference (0.0229). After 40 days, all wounds were completely healed. No statistically significant differences were found between the histological parameters assessed in the experimental and control groups. The cellulosic polysaccharide film integrated well with the tissue showing high biocompatibility and low skin reactivity.

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Experimental model of double wounds on the rats back , in order to study the skin cicatrization process on rat treated with cellulose coat

Cited by 4 publications

References 1 publication

Natural Film Based on Pectin and Allantoin for Wound Healing: Obtaining, Characterization, and Rat Model

Natural Film Based on Pectin and Allantoin for Wound Healing: Obtaining, Characterization, and Rat Model

Avaliação e caracterização proteica do muco de Phyllocaulis boraceiensis sobre a capacidade proliferativa de fibroblastos, células endoteliais e em modelos de cicatrização.

Biocompatibility and cutaneous reactivity of cellulosic polysaccharide film in induced skin wounds in rats

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