Modelo experimental de esplenectomia laparoscópica em ratos

Costa, Vanessa Alves; Oliveira, Fabrício Mascarenhas de; Jr, Lúcio Couto de Oliveira; Carreiro, Mario Castro; Guimarães, Pedro

doi:10.1590/s0102-86502003000600013

Cited by 2 publications

(1 citation statement)

References 12 publications

(15 reference statements)

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“…Regarding the parameters used in laparoscopy 14 , to develop a model of splenectomy in rats, used to establish pneumoperitoneum pressure of 7 mmHg and introduced a 5mm…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

Lessa

Carvalho²,

Spagnolo³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To investigate the development of a laparoscopy technique for local injection into the X-linked muscular dystrophy (mdx) diaphragm. METHODS:It was used 10 mice Balb/C57 and 5 mdx mice and three differents decubitus type were tested: the right lateral, supine, and supine decubitus with 20 degrees elevation of the forelimb. Abdominal caudal face and the 10 intercostal space were tested as spot to introduce the needle into the diaphragm. RESULTS:Supine position with elevation of 20 degrees forelimb and the 10th intercostal space are the beneficial position to apply a local injection. CONCLUSION:It was proved to be possible to perform the laparoscopy technique in the X-linked muscular dystrophy diaphragm and this requires a specific position and technique during the surgery.

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“…Regarding the parameters used in laparoscopy 14 , to develop a model of splenectomy in rats, used to establish pneumoperitoneum pressure of 7 mmHg and introduced a 5mm…”

Section: Discussionmentioning

confidence: 99%

Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

Lessa

Carvalho²,

Spagnolo³

et al. 2014

Acta Cir. Bras.

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Subtotal laparoscopic splenectomy in rats with preservation of the inferior pole

Silva

Paulo

2011

Acta Cir. Bras.

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Purpose:To evaluate the feasibility and safety of subtotal splenectomy by laparoscopy with inferior pole preservation and to determine the viability of the splenic remnant. Methods: Twenty male Wistar rats weighing between 365 g and 474 g (mean 417.92 ± 36.15g SD) were operated and randomly assigned to two groups : Group 1 rats were killed on postoperative day 10 (n = 10), and Group 2 on the postoperative day 80 (n = 10). Both the inferior splenic pole and the superior part of the spleen, which was used as a control to measure inferior pole viability, were weighed and morphologically examined. Results: The technique was feasible in all cases. There were two postoperative deaths on immediate postoperative and one not well defined. The average weight percentage of the inferior pole, measured indirectly on the 10th day, was 53.67% ± 11.59% and on the 80th day was 62.69% ± 6.89%. The inferior pole was necrotic, with abscess formation in one case (5.9%) and appeared normal in all other cases. Microscopy showed features that were compatible with normality. Conclusions: Subtotal splenectomy with preservation of the inferior pole by laparoscopy was feasible and safety. The lower splenic pole was viable in 94.1% of animals. Key words: Spleen. Splenectomy. Laparoscopy. Rats. RESUMOObjetivo: Avaliar a exequibilidade e a segurança da esplenectomia subtotal com preservação do polo inferior do baço por videolaparoscopia, e a viabilidade do referido polo. Métodos: 20 ratos Wistar, machos, pesando entre 365g e 474g (MA 417,92 ± 36,15g) foram operados e distribuídos em dois grupos de acordo com a época da retirada do polo inferior para estudo: grupo 1 do 10º dia, (n= 10), grupo 2, do 80º dia (n=10). A porção superior do baço que serviu de controle da viabilidade do pólo inferior e esse remanescente, retirados no término do experimento, foram medidos, pesados e enviados para exame morfológico. Resultados: A técnica foi exeqüível em todos os casos. O peso médio percentual do polo inferior avaliado de forma indireta no 10º dia foi 53,67% ± 11,59%, e no 80º dia 62,69% ± 6,89%. Houve dois óbitos após a cirurgia, provavelmente por mal condução da anestesia e/ou do pneumoperitônio, e um tardiamente por causa desconhecida. O exame macro e microscópico do polo inferior nos 17 animais sobreviventes e no que faleceu tardiamente mostrou necrose em um caso (5,9%). Conclusões: A esplenectomia subtotal com preservação do pólo inferior por videolaparoscopia em ratos foi exequível e segura. O polo inferior do baço mostrou-se viável em 94,1% dos animais. Descritores: Baço. Esplenectomia. Laparoscopia. Ratos.

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Modelo experimental de esplenectomia laparoscópica em ratos

Cited by 2 publications

References 12 publications

Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

Laparoscopic guided local injection in the X-linked muscular dystrophy mouse (mdx) diaphragm. An advance in experimental therapies for Duchenne Muscular Dystrophy

Subtotal laparoscopic splenectomy in rats with preservation of the inferior pole

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