Efeitos metabólicos da glutamina em ratos submetidos à queimadura por água fervente (escaldadura)

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PURPOSE: Development of an improved animal model for studying skin burns in rats. METHODS: Twenty-four male Wistar rats were randomly assigned to four groups (n=6): G1-Control, G2-T100°C, G3-T150°C and G4-T200°C. Two 10 x 10 mm squares were outlined with a sterile surgical marker on each side and along the vertebral column using a prepared template positioned between the anterior and posterior limbs. G2-G4 rats were subjected to 100°C, 150°C and 200ºC thermal burns, respectively. G1 rats served as controls. Burns were inflicted by applying a copper plate connected to an electronic temperature controlling device to the dorsal skin of anesthetized rats. Four burns were produced on each animal (total area: 4 cm 2 / animal) leaving about 1 cm of undamaged skin between burn areas. Analgesia was administered during 24 h after burn injury by adding 30 mg codeine phosphate hemihydrate to 500 ml tap water. RESULTS:The application of 100°C and 150ºC resulted in partial thickness skin burns with central reepithelialization of the burned area only at 100°C. In G4 group the whole thickness of the skin was injured without central reepithelialization. However, there was marginal reepithelialization in all groups. CONCLUSION: The model studied is inexpensive and easily reproducible, enabling the achievement of controlled burns with partial or total impairment of the skin in experimental animals. Keywords: Models, Animal. Hot Temperature. Burns. Rats. RESUMO OBJETIVO: Desenvolvimento de um modelo animal aperfeiçoado para estudo de queimaduras cutâneas em ratos. MÉTODOS: Vinte e quatro ratos Wistar, machos, foram distribuídos aleatoriamente em quatro grupos (n=6): G1-Controle, G2-T100°C, G3-T150°C e G4-T200°C. Dois quadrados medindo 10x10 mm foram delineados com um marcador cirúrgico estéril em cada lado e ao longo da coluna vertebral e posicionados entre os membros anteriores e posteriores, utilizando um molde previamente preparado. Os ratos dos grupos G2-G4 foram submetidos a queimaduras térmicas de 100°C, 150°C e 200°C, respectivamente. O grupo G1 foi utilizado como controle. As queimaduras foram infligidas pela aplicação de uma placa de cobre, ligada a um dispositivo de controle eletrônico de temperatura, na pele dorsal de ratos anestesiados. Quatro queimaduras foram produzidas em cada animal (área total: 4 cm2/animal), deixando cerca de 1 cm de pele intacta entre as áreas queimadas. Analgesia foi obtida durante 24 horas após a queimadura por adição de 30mg de fosfato hemi-hidratado de codeína a 500 ml de água potável. RESULTADOS: A aplicação 100°C e 150°C resultou na produção de queimaduras profundas comprometendo parte da espessura da pele, com reepitelização central da área queimada, somente a 100°C. No grupo G4 houve lesão de toda a espessura da pele sem reepitelização central. Entretanto, observou-se reepitelização marginal em todos os grupos estudados. CONCLUSÃO: O modelo estudado é de baixo custo e facilmente reproduzível, propiciando a obtenção controlada de queimaduras com comprometimento parcial ou total da...

Section: Discussionmentioning

confidence: 99%

An optimized animal model for partial and total skin thickness burns studies

Campelo¹,

Campelo²,

Britto³

et al. 2011

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“…Hence, it is necessary to accelerate the healing process to minimize tissue loss and malnutrition. In this study, the burns covered 30% of the body surface and can be considered as extensive and with systemic commitment 15,18 . The BG group was the only one to significantly lost weight along the 15 days.…”

Section: Discussionmentioning

confidence: 99%

“…Then, the template containing the animal was immersed for 10 s into water at 100ºC. This procedure resulted in non-lethal third-degree burns 15 . After being burnt, the animals, including those in the CG, were given analgesia orally using Tramadol ® (5 mg/kg), which was administered via a gavage tube every 12 h for 5 consecutive days.…”

Section: Burn Protocolmentioning

confidence: 99%

Effect of low-intensity therapeutic ultrasound on wound healing in rats subjected to third-degree burns

Mesquita

Silva

et al. 2016

PURPOSE:To determine the effectiveness of low-intensity therapeutic ultrasound (LITUS) on wound healing in rats with third-degree burns. METHODS:Twenty rats were divided into the Control Group that comprised four rats without third-degree burns that did not undergo LITUS, the Burned Group (BG), comprising eight rats with third-degree burns that did not undergo LITUS, and the Burned with Treatment Group (BTG), comprising eight rats with third-degree burns that were administered LITUS. LITUS began 24 h after injury and involved daily applications for 8 min at 0.1 W/cm 2 for 14 days. RESULTS:The BTG lost less weight than the BG (Q=2.75; p<0.05). No visible differences were apparent among the groups' lesions on day 4. By the end of treatment, wound healing was more evident in the BTG. No statistically significant differences were found between the BG and the BTG in relation to the parameters measured using the histological changes in burn wound healing scoring system. CONCLUSION:The LITUS protocol applied to the animals with third-degree burns accelerated the formation of fibrin-leukocyte crusts and significantly reduced weight loss. However, burn wound healing was not accelerated.

“…Experimental model of burns in animals have been an essential tool for the study of the pathophysiology of skin lesions that may also occur in humans, which may help to develop new methods of treatment [24][25] .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, these animals have been used in various experimental models to evaluate treatment of burns [25][26][27][28][29] .…”

Section: Discussionmentioning

confidence: 99%

Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating NF-kB and Ki-67

Campelo

Brito³

et al. 2015

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PURPOSE:To examine the effects of the oil mixes (ω-9, ω-6 and ω-3) in rats subjected to thermal burn. It was also aimed to assess whether the sources of ω3 would interfere with the effect of such mixes on the thermal injury. METHODS:Thirty-six rats distributed into five groups: burned + water, burned + isolipid mix, burned + oil mix 1 (ALA), burned + oil mix 2 (ALA + EPA + DHA of fish) and burned + oil mix 3 (ALA + DHA from seaweed). The thermal injury was involving total thickness of skin. After the burns animals received the oil mixes for seven days. The lesions were evaluated by immunohistochemistry.RESULTS: Animals receiving mix 3 showed a smaller extension of the thermal injury as compared to those that were supplemented with other oils mixes. Expression of Ki-67 in the receiving Mix 3 increased as compared to all the other groups. Animals supplemented with mix 3 were able to inhibit NF-κB in injured tissue. CONCLUSION:Rats received oil mix in which the source of ω3 (ALA+DHA of seaweed) showed inhibition of NF-κB, increase in cell proliferation, and reduction the extension of thermal lesion.Key words: Burn. Fatty Acids. Seaweed. Docosahexenoic Acid. alpha-Linolenic Acid. Rats.Oil mixes omega 9, 6 and 3, enriched with seaweed, promoted reduction of thermal burned modulating