Edema quantification by computerized morphometry as an evaluation parameter for the resistance of colon anastomoses

Priolli, Denise Gonçalves; Margarido, Nelson Fontana; Rotta, Carlos Mateus; Stephani, Simone Moreira

doi:10.1590/s0102-86502003000500005

Cited by 8 publications

(9 citation statements)

References 13 publications

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“…Variables that can be quantitatively evaluated, such as colon crypt length, thicknesses of the different layers of the intestinal wall and number of caliciform cells can be determined objectively, thereby enabling conclusions that are more precise. Computer-assisted analysis has been used with various objectives and, in relation to conventional methods, it has the advantage of enabling fast and objective quantitative evaluation of the microscopic structures 22,23 . Few studies have evaluated inflammatory alterations in the colon wall of intestinal segments that have been taken out of function, by means of morphometric analysis methods 9,11,15 .…”

Section: Discussionmentioning

confidence: 99%

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

et al. 2008

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Purpose:To evaluate histopathological alterations of the colon wall in segments with and without intestinal transit, by computer-assisted imaging, and to correlate these with the length of time diversion. Methods: Thirty male Wistar rats were subjected to intestinal transit diversion by a proximal colostomy and distal mucosa fistula. The animals were divided into three experimental groups according to how long after the initial surgical procedure they were sacrificed: six, twelve and eighteen weeks. Colon segments with and without transit were subjected to histopathological study. The variables colon crypt length, mucosal ulceration, muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria, vascular congestion, number of caliciform cells, inflammatory grade and degree of inflammation, comparing the two colon segments in the different experimental groups were studied. Intestinal crypt length, muscle layer thickness of the mucosa, submucosa and muscularis propria and caliciform cells were measured by computer-assisted imaging method. Mean equality, variance analysis and correlation tests were used in the statistical analysis, and the significance level was set at 5%. Results:Comparison between segments with and without transit showed that the latter presented reduced length of colon crypts and increased muscle layer thickness of the muscularis mucosa, submucosa and muscularis propria. There were greater quantities of ulceration of the mucosal and greater degree of inflammation with increasing time without transit. Mucosal ulceration, submucosal vascular congestion, increased thickness of the submucosal and muscularis propria layers, presence of caliciform cells, inflammatory infiltrate and inflammatory grade correlated significantly with the length of time without transit. Conclusions: Histological alterations occurred in all layers of the colon wall, in the segments without intestinal transit. Ulcerations in the intestinal mucosa, increased number of caliciform cells, greater vascular congestion of the submucosal layer and inflammatory reaction were related to increasing length of time without transit. Key words: Colon. Colitis. Colostomy. Image Processing, Computer-Assisted. Rats. RESUMOObjetivo: Avaliar por método de imagem assistida por computador as alterações histopatológicas da parede cólica em segmentos providos e desprovidos de trânsito intestinal e relacioná-las ao tempo de exclusão. Métodos: Trinta ratos Wistar machos foram submetidos à derivação do trânsito no cólon esquerdo por meio de colostomia proximal e fístula mucosa distal. Os animais foram divididos em três grupos experimentais segundo o sacrifício ter sido realizado seis, doze e dezoito semanas após o procedimento cirúrgico inicial. Segmentos dos cólons providos e desprovidos de trânsito foram submetidos a estudo histopatológico. Foram analisadas as variáveis: comprimento das criptas cólicas, ulceração na mucosa, espessura das camadas muscular da mucosa, submucosa e muscular própria, congestão vascular, número...

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Section: Discussionmentioning

confidence: 99%

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

et al. 2008

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“…With the advent of computer-assisted image analysis software, it has become possible to objectively quantify the tissue expression of various proteins, stained using histochemical or immunohistochemical techniques 16,17 . With this method, it has become possible to quantify the expression of sulfomucins and sialomucins in the colon mucosa more precisely, thereby observing changes in total acid mucin content and the influence of the duration of exclusion, by comparing segments with and without fecal stream.…”

Section: Introductionmentioning

confidence: 99%

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

et al. 2010

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PURPOSE: To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion. METHODS: Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05). RESULTS: There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion. CONCLUSIONS: Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.

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“…Little space is needed to house them; procreation occurs in large numbers and rapidly, ensuring almost identical lineages with animals that are genetically and physiologically similar. The management and maintenance costs are small compared with those for other animals; they are easy to obtain and handle, and are resistant animals 5 . In addition to these characteristics, it has been accepted since the study by Herrmann in 1964 6 that the fecal microflora of rats is very similar to that of humans.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have habitually used animals of male gender [1][2][3][4][6][7][8][10][11][12][13]16,18 . even though there is no restriction on using females, purely from the point of view of location of the PP 5,14,21 .…”

Section: Discussionmentioning

confidence: 99%

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Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

et al. 2009

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PURPOSE: To validate Peyer's patch as an anatomical repair landmark for colorectal surgery in rats and to measure the collagen content in segments of the colon containing or not containing Peyer's patch. METHODS: The distance between Peyer's patch and the peritoneal reflection was measured in forty-five Wistar rats. The colon and rectum were resected for quantification of collagen content by means of computer-assisted image analysis in regions of the colon with and without Peyer's patch. RESULTS: There was great variation in the distance between Peyer's patch and the peritoneal reflection when the male and female rats were considered as a single group (p=0.04). Comparison between the genders showed that the distance between the patch and the peritoneal reflection was greater in female than in male rats (p=0.001). The colonic segment containing Peyer's patch was observed to have lower tissue collagen content than the segment in which this structure was not present (p=0.02). CONCLUSION: Peyer's patch can be indicated as an anatomical repair landmark, and there is a need to study the healing of colorectal anastomoses in rats based on differing quantities of tissue collagen existing in the colonic wall with or without this structure.

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Edema quantification by computerized morphometry as an evaluation parameter for the resistance of colon anastomoses

Cited by 8 publications

References 13 publications

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

Evaluation by computerized morphometry of histopathological alterations of the colon wall in segments with and without intestinal transit in rats

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

Is peritoneal reflection the best anatomical repair landmark in experimental colorectal surgery on rats?

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