2003
DOI: 10.1590/s0102-86502003000400017
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Abstract: Experimental models of organ transplantation played a crucial role to establish the principles of transplantation immunology. The renal transplantation in rodents became the most used model to study the mechanisms of allograft rejection. To perform it, it is necessary to master the microsurgery techniques and the research group should cooperate with other specialists in the field. In this article we review the surgical techniques employed in rats, and we draw guidelines to establish studies about transplantati… Show more

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Cited by 5 publications
(2 citation statements)
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References 10 publications
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“…In this model also, vascular anastomosis was performed using the cuffs mentioned above. It was also possible to use the cuffs in the rat kidney transplantation model 31,32 . The cuff method can be applied for many different organ transplantations, but essentially it is a foreign body, and it is not suitable for long-term studies extending over several months.…”
Section: Technologies For Beginners and Intermediate Researchersmentioning
confidence: 99%
“…In this model also, vascular anastomosis was performed using the cuffs mentioned above. It was also possible to use the cuffs in the rat kidney transplantation model 31,32 . The cuff method can be applied for many different organ transplantations, but essentially it is a foreign body, and it is not suitable for long-term studies extending over several months.…”
Section: Technologies For Beginners and Intermediate Researchersmentioning
confidence: 99%
“…After recipient nephrectomy, left kidney grafts were implanted orthotopically using standard microsurgical techniques (an end-to-end anastomosis using polypropylene 10-0 suture). 3 Then, graft perfusion was assessed during the first 10 min after releasing vascular clamps.…”
Section: Animals and Experimental Designmentioning
confidence: 99%