Uso do propofol (2,6 diisopropilfenol) como inibidor da lesão tecidual na isquemia e reperfusão mesentérica: estudo experimental em ratos

Purpose:To evaluate the antioxidant effect of Propofol and N-Acetylcysteine (NAC) on intestinal ischemia/reperfusion (I/R) in rats by determining carbonyl protein level. Methods: Forty Wistar rats were randomly assigned into the following groups: Control; Sham; I/R with Propofol; I/R with Propofol and NAC; I/R with Ketamine and Xylazine. The I/R groups underwent 60 minutes of ischemia and an equal period of reperfusion. Blood samples, collected by cardiac punction, were centrifuged for plasma obtainment. Protein carbonyl level in plasma samples was determined by immunoblotting. Results: No significant difference in protein carbonyl level was found between Control and Sham groups (P>0.05). The highest reduction in protein carbonyl level (P<0.05) was obtained with the administration of Propofol and NAC (Group 4) in intestinal I/R procedure. Conclusion: The administration of Propofol and NAC showed the best antioxidant effect on oxidative stress in rats that underwent intestinal I/R procedure, suggesting a synergistic interaction.

Section: Discussionmentioning

confidence: 99%

Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: Protein carbonyl detection by immunoblotting

Azeredo¹,

Azeredo²,

Eleuthério³

et al. 2008

“…This knowledge has led many researchers to search for a method capable of minimizing the damage caused during reperfusion, and highlights the use of some drugs for this purpose 5 . However, there is no medicine to benefit large enough to use in clinical practice for patients with ischemia 6 .…”

Section: Introductionmentioning

confidence: 99%

Postconditioning effect in the hepatic ischemia and reperfusion in rats

Santos¹,

Pontes²,

Miiji

et al. 2010

Self Cite

Purpose:To evaluate the effect of postconditioning protection in liver tissue of rats submitted to ischemia and reperfusion. Methods: 25 Wistar male rats were randomized in three groups: Group A (ischemia and reperfusion -I/R), with 10 rats, which was made ischemia by vascular clamp application in hepatic hilum for 30 minutes and reperfusion for 60 minutes by removal of the clamp; Group B (Postconditioning -IPo), with 10 rats, with same procedure plus postconditioning (3 cycles of reperfusion inserted by 3 cycles of ischemia, 30 seconds for each phase, between ischemia and reperfusion phases); and Group C (Sham), with 5 rats, which took place only laparotomy and manipulation of the hepatic hilum. Specimens were examined (histological evaluation) and dosage of serum AST and ALT was made. The statistical analysis was made with t Student test, with significant difference when p<0.05. Results: In the Sham group there was no histological or enzymatic changes; In group A the mean tissue lesion was 1.6 and in group B 1.1 (p=0.014). In group A the mean ALT level was 355U/l and in group B was 175.9U/l (p=0.016). The mean of AST was 828.8U/l in group A and 295.5U/l in group B (p=0.001). Conclusion: The postconditioning was able to minimize the severity of liver injury in rats submitted to ischemia and reperfusion. Key words: Ischemia. Reperfusion. Liver. Rats. RESUMO Objetivo:Avaliar o efeito do pós-condicionamento isquêmico na proteção tecidual hepática de ratos submetidos à isquemia e reperfusão. Métodos: Foram utilizados 25 ratos da linhagem Wistar provenientes do Biotério da Universidade Federal de Mato Grosso do Sul, distribuídos em três grupos: A) grupo isquemia/reperfusão (I/R) (10 ratos), em que foi realizada isquemia e reperfusão hepática pela aplicação e retirada de clampe do hilo hepático durante 30 e 60 minutos, respectivamente; B) grupo pós-condicionamento isquêmico (PoCI) (10 ratos), em que foi realizado procedimento semelhante ao anterior, exceto pela realização do pós-condicionamento isquêmico entre as fases de isquemia e reperfusão, consistindo na retirada e colocação do clampe do hilo hepático por 30 segundos cada fase, durante 3 ciclos; grupo C (Sham) (5 ratos), em que realizou-se apenas laparotomia e manipulação do hilo hepático. Foram analisados os espécimes (avaliação histológica), dosagem sorológica de AST e ALT. Resultados: No grupo Sham não se observou alterações histológicas nem enzimáticas; no grupo A a média de lesão tecidual foi 1,6 e no grupo B 1,1 (p=0,014). No grupo A a média de ALT foi 355U/l e no grupo B 175,9U/l (p=0,016). A média de AST foi de 582,8U/l no grupo A e 295,5U/l no grupo B (p=0,001). Conclusão: O pós-condicionamento isquêmico foi capaz de minimizar a intensidade das lesões hepáticas de ratos submetidos à isquemia e reperfusão. Descritores: Isquemia. Reperfusão. Fígado. Ratos.

“…The mesenteric blood flow reduction and the ischemiareperfusion injury plays an important role in the pathogenesis and survival of many clinical and surgical diseases [9][10][11] , with important research approach done [12][13][14][15] to understand the involved mechanisms aiming to obtain the best protection. Although confirmed in many researches, the pre and postconditioning failure to avoid the ischemia and ischemia-reperfusion lesions is also reported [16][17][18][19] , raising the respiratory dysfunction after cardiopulmonary bypass 20 and to brain stroke events when induced by proton pump Na This relative contradictory scenario regarding results with ischemic and or drug induced preconditioning was consistently recently studied by Ramzy et al 22 pointing the importance and benefits of new researches for best clinical results with pre and postconditioning.…”

Section: Discussionmentioning

confidence: 99%

Ischemia-reperfusion histopathology alterations of the rabbit intestinal wall with and without ischemic preconditioning

Gomes¹,

Filho

Salles

et al. 2011

Self Cite

. Provided institutional support for the research development. ABSTRACT Purpose:To evaluate the histopathology alterations of the intestinal mucosa of rabbits submitted to mesenteric artery ischemia and reperfusion with and without ischemic preconditioning. Methods: Two groups of ten male New Zealand white rabbits body (weight 2.2-3.0, average 2.5 kg). For mesenteric ischemia induction in all animals the small bowel and mesentery were cut 30cm and 60cm far from the gastroduodenal pyloric transition before the proximal mesenteric artery occlusion. In the Group 1 animals, the proximal mesenteric artery was occluded for 45 min with an atraumatic vascular clamp, followed by reperfusion for 30 min. In the Group 2 the 45 min ischemic phase was preceded by three cycles of ischemia (2 minutes each) alternated with three cycles of reperfusion (2 minutes each). For istopathology study small bowel biopsies were obtained before ischemia (control), after 45 min of mesenteric ischemia and at 30 min. of mesenteric artery reperfusion. Results: In the Group I animals, the followings histopathology grade results were observed: t1, mean 2,8; t2, mean 3,3. Using the Kruskal-Wallis non-parameter test, differences between t0 and t1 and t0 and t2 were significants (p<0.05), but not significant between t1 and t2 (p>0.05). In the Group 2 animals histopathology grade results were: t1 mean 2,6 and t2, mean 2,1. Differences between t0 and t1, t0 and t2 were significant (p<0.05). It was not observed differences (p>0.05) between results of t1 in both groups but histopathology injury observed in Group 1 t2 biopsies were higher (p<0.05) than observed in the same period (t2) of Group 2 animals. Conclusion: Microscopic examination of the biopsies revealed significant evidence of preconditioning protection against small bowel wall ischemia-reperfusion injury. Key words: Mesenteric Vascular Occlusion. Ischemic Preconditioning. Animal Experimentation. Rabbits. RESUMOObjetivo: Avaliar as alterações histopatológicas da mucosa intestinal de coelhos submetidos a isquemia-reperfusão com e sem precondicionamento isquêmicol. Métodos: Foram estudados dois grupos de dez coelhos Nova Zelândia machos com pesos variáveis entre 2,2 e 3,0 kg (média de 2,5 kg) de peso corpóreo. Para indução da isquemia, em todos os animais, o intestino delgado e o mesentério foram seccionados 30 cm e 60 cm após a transição pilórica gastroduodenal, antes da oclusão da artéria mesentérica cranial. Nos animais do Grupo 1, a artéria mesentérica proximal foi ocluida por pinçamento atraumático durante 45 min., seguido de reperfusão por 30 min. No Grupo 2, foi realizado precondicionamento por três ciclos de 2 min. de oclusão mesentérica intercalados com três ciclos de 2 min. de reperfusão, seguido de oclusão mantida por 45 min e reperfusão de 30min. como no Grupo I. Para estudo histopatológico, foram obtidas biópsias da parede intestinal antes da isquemia (t 0 -controle), após 45 min. de isquemia (t 1 ) e após 30 min. de reperfusão (t 2) . Resultados: No Grupo I foram observados os segu...