Comparação entre pericárdio bovino preservado em glicerina e malha de poliéster no reparo de falhas da parede abdominal em ratos

Quitzan, Juliany Gomes; Rahal, Sheila Canevese; Rocha, Noeme Souza; Crocci, Adalberto José

doi:10.1590/s0102-86502003000400007

Cited by 14 publications

(30 citation statements)

References 5 publications

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“…Os animais que receberam o pericárdio bovino tratado com glicerina, no presente trabalho, apresentaram perda do formato original do implante desde o primeiro período de análise, como constatado em estudos prévios [2,3,18]. Entretanto, nossos resultados mostraram que quanto maior a concentração de glutaraldeído utilizada no tratamento e conservação da membrana, maior a dificuldade do organismo para incorporá-la, uma vez que a reação inflamatória produzida e a resistência da membrana foram mais intensas.…”

Section: Discussionunclassified

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Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

Costa

Silva²,

Andrade

2016

Acta Scientiae. Vet.

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Background: Lately, the use of biological materials has been widely indicated in surgical procedures to restore structure and function of injured tissues. Bioimplants require different conservation techniques; among these, glutaraldehyde preservation stands out owing to its higher antimicrobial efficiency as compared to glycerin. In view of the need to determine a concentration of glutaraldehyde that can act as a biocide but do not cause undesirable tissue reactions, this study aimed to identify and quantify gross and microscopic tissue alterations after implantation of bovine pericardium, which was preserved in various concentrations of glutaraldehyde, in the abdominal wall of mice.Materials, Methods & Results: Fresh pericardia from 18 bovines were fractioned into 1cm2 samples and treated with a 98% glycerin solution for 30 days (control group), or 0.625%, 1%, and 1.5% glutaraldehyde solution for 18 days (experimental groups). An abdominal muscle fragment was excised from each mouse, and a 1-cm2 fragment of preserved pericardium was implanted in the area. Sixty mice (n = 15 per treatment) divided into groups were observed for 7, 14, and 30 days, and five animals from each group were euthanized at each time point for gross and microscopic examination. Fragments of the implants and adjacent skin lesions were harvested, fixed in formalin, and processed for routine histology and microscopic analysis. Both the type of inflammatory infiltrate and the repair process of the tissue response were similar between the groups that received glycerin-preserved pericardium and those that were subjected to pericardium preserved with 0.625% glutaraldehyde. Animals that received 1% glutaraldehyde-preserved implants and were examined 30 days thereafter exhibited a chronic, intense reaction with fibrosis and necrosis of the abdominal wall muscles, as well as calcification and presence of giant cells, when compared to the animals examined at 7 and 14 days in the same treatment group. These changes were also present and more intense in animals that received 1.5% glutaraldehyde-preserved pericardium examined at 14 and 30 days later, with tissue destruction and impaired incorporation of the implant into the adjacent muscle tissue.Discussion: The continuous cell destruction observed in animals treated with implants preserved with 1% or 1.5% glutaraldehyde is a hallmark of chronic inflammation, since several inflammatory cell molecules contribute to this lesion. A cycle is created: continuous degradation sustains inflammation, and inflammatory molecules contribute to the process of cell destruction. Consequently, we conclude that the use of glutaraldehyde at concentrations of 1 or 1.5% is not feasible for preservation of biological materials. Tissue repair was chronologically more effective in the group treated with glycerinpreserved implants, since animals treated with glutaraldehyde-preserved implants needed a longer period to restore due to presence of a persistent inflammatory response, immunogenicity, calcification, and deficient remodeling. The ideal preservative for biological materials should not cause chronic and/or intense inflammatory reaction in order to preserve the implant’s structure and allow its perfect incorporation into the tissue, even if the chosen preservative is flexible and exhibits disinfectant properties. Therefore, we conclude that glutaraldehyde at concentrations of 0.625% to 1% is suitable as a preservative for biomaterials because the tissue reaction it causes is tolerable; additionally, glutaraldehyde at concentrations close to 1% has been described to have sterilizing properties.

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Section: Discussionunclassified

“…Embora esta substância promova uma excelente incorporação do implante tratado, apresenta eficácia antimicrobiana reduzida [18]. Desde então, a preocupação com a solução ideal e sua respectiva concentração se tornaram assunto de muitas pesquisas [1,5,11,14,25].…”

Section: Introductionunclassified

Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

Costa

Silva²,

Andrade

2016

Acta Scientiae. Vet.

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“…Gore-Tex® is among the synthetic fabrics that are frequently utilized to repair defects in human beings by providing greater accumulation of neutrophils [13]. Given its easy handling, flexibility, readily obtainable, good adherence; it is used as support for the growth of fibrous tissue and doesn't set off infection signs [14].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Bovine Pericardium and Gore-Tex in Tissual Interaction with Wistar Rats Diaphragm

Guerra¹,

Filho²,

Novo³

et al. 2016

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“…A substituição de tecidos orgânicos em perdas estruturais anatômicas decorrentes de má formação congênita ou traumatismos compreende um dos principais desafios da cirurgia, sendo empregados diferentes materiais para esse fim, sejam sintéticos ou biológicos (Santillán-Doherty et al, 1995;Quitzan et al, 2003). Em medicina veterinária, utilizam-se, frequentemente, os implantes biológicos, especialmente na reconstrução dos músculos do diafragma e da parede abdominal (Cárdenas-Laislon et al, 1997;Mazzanti et al, 2000).…”

Section: Introductionunclassified

“…No tratamento de hérnias, sobretudo as recorrentes com ausência de tecido íntegro para fechamento do anel herniário, além das membranas sintéticas, como as telas de polipropileno, poliéster e poliuretano (Quitzan et al, 2003), o emprego de implantes biológicos conservados é relativamente comum (Shoukry et al, 1997;Raiser et al, 2001;Rabelo et al, 2004).…”

Section: Introductionunclassified

Hernioplastia experimental em coelhos por meio de cartilagem auricular bovina conservada em glutaraldeído

Silva

Franco

Menezes

et al. 2009

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliou-se a viabilidade do emprego da cartilagem auricular bovina conservada em glutaraldeído a 4% na hernioplastia experimental, empregando-se seis coelhos adultos, machos, da raça Nova Zelândia. Para obtenção da hérnia incisional, removeu-se um segmento elíptico de 3cm de comprimento por 1cm de largura, no ponto central à primeira incisão, envolvendo fáscia e tecido muscular, na região da cicatriz umbilical. Dois animais de cada vez foram sacrificados aos 15, 30 e 45 dias após a cirurgia. Nos sacrificados aos 15 dias, observaram-se áreas focais de inflamação, caracterizadas por abscesso e fístula. À microscopia, observou-se área de inflamação e necrose próxima à periferia do implante. Nas amostras colhidas dos animais sacrificados aos 30 dias, não foram evidenciadas alterações clínicas relevantes. Desses, um animal apresentou à microscopia intensa proliferação fibroblástica, moderada neovascularização e células inflamatórias predominantemente mononucleares. Dos sacrificados aos 45 dias, em um ocorreu aderência de alça intestinal ao implante. É possível inferir que o material implantado apresentou satisfatória compatibilidade com o tecido receptor. Conclui-se que o implante de cartilagem auricular bovina conservada na hernioplastia experimental em coelhos apresentou evidências de boa integração tecidual e cicatrização, não havendo eliminação do material implantado. Palavras

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Comparação entre pericárdio bovino preservado em glicerina e malha de poliéster no reparo de falhas da parede abdominal em ratos

Cited by 14 publications

References 5 publications

Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

Pathological Features of Bovine Pericardium Implanted Into Mice Abdominal Wall after Preservation with Glutaraldehyde or Glycerin

Comparative Study of Bovine Pericardium and Gore-Tex in Tissual Interaction with Wistar Rats Diaphragm

Hernioplastia experimental em coelhos por meio de cartilagem auricular bovina conservada em glutaraldeído

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