Effects of alpha-tocopherol on bacterial translocation and lipid peroxidation in rats with intestinal obstruction

Abstract

PURPOSE: Investigate if alpha-tocopherol has a protective effect on intestinal mucosa after obstruction and to evaluate the potential relations between lipid peroxidation and bacterial translocation. METHODS: Ten rats were submitted to a sham laparotomy and six served as control group. A small bowel obstruction was done in sixteen animals and among them eight were pretreated with alpha-tocopherol. Forty-eight hours later, mesenteric lymph node, spleen, liver and blood cultures and also samples from ileal mucosal were obtained, Thiobarbituric acid reactive substances (TBARS) levels were determined and intestinal histological assessment was performed. RESULTS: Bacterial translocation was significantly increased in the obstructed rats compared with the control, sham and antioxidant pretreated groups (p< 0,05). TBARS (nmol/100mg) in untreated obstructed rats increased from 49,0 ± 13,3 in control group to 128,8 ± 40 after 48 hours of intestinal obstruction and achieved 72,3 ± 24,6 in alpha-tocopherol group (p< 0,05). Bacterial adherence to the intestinal epithelial cells surface and mucosal necrosis were significantly increased in the obstructed compared with nonobstructed rats. CONCLUSION: Alpha-tocopherol reduce the deleterious effects of the TBARS over the intestinal mucosal suggesting that in such circumstances there might be an association between bacterial translocation and lipid peroxidation after an intestinal occlusion.

Vaginal lipidomics of women with vulvovaginal candidiasis and cytolytic vaginosis: A non-targeted LC-MS pilot study

“…Furthermore, tocopherols are lipids associated with the cell membranes, acting against the most reactive forms of free radicals, besides preventing lipid peroxidation and bacterial translocation, hence reducing tissue injuries [ 55 – 58 ]. Our study demonstrated a higher concentration of gamma-tocopherol in the control group, which is a derivative of vitamin E. Notably, this lipid is important for the vaginal epithelial tissue homeostasis as it acts as a protector against disorders in the local microenvironment, such as the oxidative stress caused by infections and dysbioses.…”