Experimental model for endometriosis. Comparative histological study between the ectopic and eutopic endometrium

Rezende, Cézar Alencar de Lima; Silva, Alcino Lázaro da; Júnior, João Batista Novães; Gobbi, Helenice; Martins, Madalena Maria Ferreira

doi:10.1590/s0102-86501997000400003

Cited by 4 publications

(6 citation statements)

References 9 publications

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“…The morphological data we have obtained coincide with those of other researchers who used a similar technique. Thus, Elgamal et al [65] describes ectopic endometrioid lesions macroscopically in the form of cysts filled with fluid, and the histological picture obtained by us completely corresponds to the described one by Rezende et al [66], Neto et al [67] and Amaral et al [68] (Fig. 1).…”

Section: Original Researchsupporting

confidence: 85%

“…On the fourth day of the experiment, experimental induction of endometriosis was performed by means of surgery and implantation of an autologous uterine fragment using the method described by A. Golan et al [63] and T. Hirata et al [64]. After awakening from ketamine-xylazine intraperitoneal anesthesia, rats were randomly and evenly divided into groups.…”

Section: Objective Of the Studymentioning

confidence: 99%

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The effects of certain angioneogenesis inhibitors in experimental endometriosis in rats

Barbe¹,

Berbets²,

Давыденко³

et al. 2019

COT

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The pathogenetic theory of retrograde outflow of endometrial cells into the peritoneal cavity at endometriosis is gaining an increasing support. Adequate blood supply and angioneogenesis play an important role in successful implantation and occurrence of ectopic foci. VEGF plays an important role in this process. Determination of the pathways affecting the activity of this factor seems to be promising in terms of its effect produced on the early pathogenetic links of endometriosis. OBJECTIVES: to determine the effectiveness of dopamine agonist cabergoline and highly selective COX-2 inhibitor celecoxib in an experimental model of external genital endometriosis in rats. METHODS. 83 outbred white female rats of Rattus Norvegicus Wistar were involved in the experiment. Experimental induction of endometriosis was performed by surgery and implantation of the autologous uterine fragments. The type and volume of experimental endometriosis lesions on the peritoneum of experimental animals were evaluated macroscopically as well as their hystologic examination were performed. RESULTS. Administration of a dopamine receptor agonist as a VEGF inhibitor separately was found to produce a pronounced inhibitory effect on ectopic endometrioid formation. However, the use of a dopamine receptor agonist in combination with a highly selective COX-2 inhibitor does not lead to potentiation or summation of their effects. At the same time, the use of COX-2 inhibitor alone has shown significantly lower efficacy than using the dopamine receptor agonist as a VEGF inhibitor. CONCLUSION. At experimental endometriosis in rats, dopamine agonists and highly selective COX-2 inhibitors were found to be one of VEGF inhibitors available.

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Section: Original Researchsupporting

confidence: 85%

Section: Objective Of the Studymentioning

confidence: 99%

The effects of certain angioneogenesis inhibitors in experimental endometriosis in rats

Barbe¹,

Berbets²,

Давыденко³

et al. 2019

COT

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“…Experimental animal models play an important role in understanding the nature of the disease [ 8 ]. The existing controversies concerning the pathophysiology of endometriosis and its influence on pregnancy and vice versa demonstrate the need for research in laboratory animals [ 9 ]. Recent studies was done on the structural and biochemical changes [ 10 ], endometrial nerve fiber [ 11 ], endometrial angiogenesis [ 12 ] and endometrial receptivity in endometriosis [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Features of Eutopic Endometrium in a Rat Model of Endometriosis

Elgamal

Othman

Ahmed

2016

J Microsc Ultrastruct

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Endometriosis is a chronic recurrent disease that is relatively common. Diagnosis is difficult and often delayed. Current treatments are inadequate with unacceptable side effects and multiple surgeries may be needed. Abnormal eutopic endometrium may play important role in endometriosis-associated infertility. This study aimed to examine the ultrastructural changes in eutopic endometrium in a rat model of surgically induced endometriosis. Endometrial tissue was removed from rats in surgical endometriosis induction group (n = 10), sham operated (n = 10) and non-operated control (n = 10) groups in the diestrus phase of the estrus cycle. They were studied with light, transmission and scanning electron microscope as well as morphometric analysis. Eutopic endometrium in surgically induced endometriosis showed pseudostratified epithelium, vacuolated columnar cells alternated with dark cells. The stroma was edematous exhibiting dilated, congested blood vessels. The mean endometrial mucosal depth and surface epithelial height significantly increased. Ultrastructurally, most luminal epithelial cells showed vacuolation. Mucous secretory granules were surrounded by dilated rough endoplasmic reticulum cisternae. Mitochondria, glycogen deposits and vesicles with electron dense cores were observed. The nuclei were highly euchromatic. Well defined microvilli were noticed with evident apical tight junctions. Scanning electron microscope revealed flattened and structurless surface epithelium with apparent decrease in the number of pinopodes. A different response to sex hormones in different parts of eutopic endometrium was observed. Ultrastructural features of estrogen dominance or progesterone resistance in the eutopic endometrium might account for inappropriate cyclic changes occurring in the disease.

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“…All the animals were examined at the same stage of the es-trous cycle by waiting 5 days after this procedure. Thereafter, an experimental model of endometriosis was created surgically using the method described by Rezende et al 4 Briefly, to create the experimental model, an approximately 1-cm-long section of the uterus was removed, primarily from the right horn of the uterus 1 cm above the cervix, and the cylinder-shaped section of the uterus was flattened. The myometrium was split from the endometrium under a microscope, and the dimensions of the 4 endometrial implants thus obtained were measured.…”

Section: The Animals and Surgical Proceduresmentioning

confidence: 99%

“…1 Histologic and cellular changes observed in eutopic and ectopic endometrial tissues from experimentally induced endometriosis are almost identical to those observed in women with endometriosis. [2][3][4] Iron levels are reportedly increased in women with endometriosis and in research animals with experimentally induced endometriosis. [5][6][7] Iron plays an important role in the progression of endometriosis because it affects cell pro liferation.…”

mentioning

confidence: 99%

In vivo effects of curcumin and deferoxamine in experimental endometriosis

Kizilay¹,

Uz²,

Şeren³

et al. 2017

Adv Clin Exp Med

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Background. Endometriosis is one of the most common chronic gynecological diseases. Objectives. The aim of the study was to examine the effects of curcumin and/or deferoxamine on cell proliferation in a rat model of endometriosis. Material and methods. Thirty female 12-week-old albino Wistar rats, weighing 200-250 g, were used in this study. All the rats underwent ovariectomy and 0.1-mg β-estradiol 17-valerate pellets were placed intraperitoneally. An experimental model of endometriosis was created in all the animals. To create the experimental model, an approximately 1-cm long section of the uterus was taken, primarily from the right horn of the uterus. Autologous fragments were then placed between the peritoneum and muscle. The animals were divided into 3 groups: Group A, treated only with the vehicle used for curcumin and deferoxamine; group B, treated with curcumin (100 mg/kg body weight); and group C, treated with deferoxamine + curcumin (100 mg/kg body weight). After biopsy samples were obtained, the sections were stained with hematoxylin and eosin. Immunostaining for cytokeratin-7 and proliferating cell nuclear antigen (PCNA) was performed. Blood iron levels were measured using a Perkin Elmer AAnalyst 800 Atomic Absorption Spectrophotometer. Results. The endometrial implant size increased in Group A, but treatment with curcumin (p = 0.01) and deferoxamine + curcumin (p = 0.007) reduced the implant size. In ectopic endometrial epithelial cells, there were significant decreases in PCNA immunoreactivity between groups A and B (p = 0.044) and between groups A and C (p = 0.033). Conclusions. Treatment with curcumin alone and/or in combination with deferoxamine contributed to a reduction in implant size and cell proliferation in a rat endometriosis model. Iron-chelating agents may act in the same manner when used in women with endometriosis; however, further studies from different perspectives are still needed.

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Experimental model for endometriosis. Comparative histological study between the ectopic and eutopic endometrium

Cited by 4 publications

References 9 publications

The effects of certain angioneogenesis inhibitors in experimental endometriosis in rats

The effects of certain angioneogenesis inhibitors in experimental endometriosis in rats

Ultrastructural Features of Eutopic Endometrium in a Rat Model of Endometriosis

In vivo effects of curcumin and deferoxamine in experimental endometriosis

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