Comparison of nerve graft integration after segmentar resection versus epineural burying in crushed rat sciatic nerves

Cunha, Marco Túlio Rodrigues da; Silva, Alcino Lázaro da; Fenelon, Sheila Bernardino

doi:10.1590/s0102-86501997000400002

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…The choice of the sciatic nerve of mice not only met the specified requirements, but was also found to be well established in the literature consulted 2,4,5,6,7,8 . The choice of this experimental model was also favoured by the diameter of the sciatic nerve and by its relatively easy surgical access on the posterior surface of the thigh of the animal in question 10,11 .…”

Section: Discussionmentioning

confidence: 90%

“…This is corroborated by the fact that it maintains the integrity of most of the basal laminae of the endoneural tubes, thus isolating the content of the tubes in relation to the adjacent tissues, thereby minimizing the effect of biological barriers, which might jeopardize the natural course of axonal growth towards the target organs 6 . The choice of the methodology was also based on studies 12,13 which reported that the most frequent lesions in sciatic nerves of humans are due to neurapraxia or axonotmesis.…”

Section: Discussionmentioning

confidence: 92%

“…The basal laminae of the endoneural tubes resist the trauma, but some of the tubes may rupture, exposing their content, with consequent loss of material and functional alterations. Animal experiments represent quite an important study model as a basis for nerve regeneration, and the sciatic nerve of the rat is the established experimental model in the investigation of peripheral nerve regeneration 2,3,4,5,6,7,8,9 . The choice of this experimental model was further justified by the fact that the sciatic nerve of a rat has a large diameter and is relatively easy to access 10,11,12,13 .…”

Section: Introductionmentioning

confidence: 99%

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Histopathological analysis of gangliosides use in peripheral nerve regeneration after axonotmesis in rats

et al. 2008

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Purpose:To analyze the action of gangliosides in peripheral nerve regeneration in the sciatic nerve of the rat. Methods: The sample was composed of 96 male Wistar rats. The animals were anaesthetized and, after identification of the anaesthesic plane, an incision was made in the posterior region of the thigh, followed by skin and muscle divulsion. The right sciatic nerve was isolated and compressed for 2 minutes. Continuous suture of the skin was performed. The animals were randomly divided into two groups: the experimental group (EG), which received subcutaneous injection of gangliosides, and the control group (CG), which received saline solution (0.9%) to mimic the effects of drug administration. Results: No differences were observed between the experimental and control groups evaluated on the eighth day of observation. At 15 and 30 days the EG showed an decrease in Schwann cell activity and an apparent improvement in fibre organization; at 60 days, there was a slight presence of Schwann cells in the endoneural space and the fibres were organized, indicating nerve regeneration. At 15 and 30 days, the level of cell reaction in the CG had diminished, but there were many cells with cytoplasm in activity and in mitosis; at 60 days, hyperplastic Schwann cells and mitotic activity were again observed, as well as nerve regeneration, but to a lesser extent than in the EG. Conclusion: The administration of exogenous gangliosides seems to improve nerve regeneration. Key words: Gangliosides. Nerve Regeneration. Sciatic Nerve. Rats. RESUMOObjetivo: Avaliar os efeitos de gangliosídeos na regeneração nervosa periférica em nervo isquiático de ratos após axonotmese. Métodos: Foram utilizados 96 ratos machos albinos (Wistar). Os animais foram anestesiados e após constatação do plano anestésico, foi realizada incisão na face posterior da coxa direita do animal. Em seguida, foi realizada a dissecção cirúrgica da pele e do músculo e divulsão dos músculos. O nervo isquiático direito foi isolado e sofreu compressão por 2 minutos. Efetuou-se a sutura contínua da pele. Os animais foram distribuídos aleatoriamente em 2 grupos: experimental (GE) que receberam gangliosídeos pela via sub-cutânea e controle (GC) que receberam soro fisiológico 0,9% com a finalidade de mimetizar os efeitos de administração da droga de estudo. A análise histopatológica foi realizada em 8, 15, 30 e 60 dias. Resultados: Não se evidenciaram diferenças significantes entre os grupos controle e experimental avaliados com 8 dias. Nos grupos experimentais de 15 e 30 dias observouse uma diminuição da atividade das células de Schwann e aparente melhora na organização das fibras nervosas; com 60 dias havia discreta presença células de Schwann no espaço endoneural e as fibras nervosas estavam organizadas sinalizando a regeneração nervosa. Nos grupos controles de 15 e 30 dias o padrão de reação celular diminuiu, entretanto havia muitas células com citoplasmas em atividade e mistose; com 60 dias observou-se ainda a presença de hiperplasia de células de Schwann, a...

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Histopathological analysis of gangliosides use in peripheral nerve regeneration after axonotmesis in rats

et al. 2008

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“…This is corroborated by the fact that it maintains the integrity of most of the basal laminae of the endoneural tubes, thus isolating the content of the tubes in relation to the adjacent tissues, minimizing the effect of biological barriers that might jeopardize the natural course of axonal growth towards the target organs 5 . The choice of methodology was also based on a study 8 that reported that the most frequent lesions in the sciatic nerves of humans are due to neurapraxia or axonotmesis.…”

Section: Discussionmentioning

confidence: 92%

Functional histopathological and morphometric study of the use of gangliosides in nerve regeneration in rats after axonotmesis

Silva-Neto

Vasconcelos

Silva

et al. 2009

International Journal of Oral and Maxillofacial Surgery

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Partial epineural burying of nerve grafts with different sizes next to or distant from neurorrhaphy’s site: histological and electrophysiological studies in rat sciatic nerves

et al. 2001

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The aim of the present study was to compare and correlate histologically and electromyographically the effects of partial epineural burying of sural nerve segments in sectioned and sutured rat sciatic nerves. Sixty adult male Wistar rats were operated on 3 groups: Group 1, sural nerve graft, 9mm long, placed next to neurorrhaphy; Group 2, sural nerve graft, 9mm long, buryied 10mm distant from neurorrhaphy; Group 3, sural nerve graft, 18mm long, set next to neurorrhaphy. The morphological features were examined at light microscope after 3 months in 45 rats. The elements observed were: vascularization, vacuoles in nerve fibers, mastocytes and inflammatory infiltrate. The morphometry was made after 6 months in 15 rats from Group 1, 2 and 3, measuring external nerve fiber diameters and counting myelinated nerve fibers/mm². The electrophysiological study was perfomed after 6 months, registering maximum amplitude and frequency of EMG pontentials, at rest, in extensor digitorum longus muscle. Group 3 rats presented sciatic nerves better conserved morphologically and mean external nerve fiber diameters greater than those from Groups 1 and 2. There were no significant differences in density of nerve fibers/mm², and in the electrophysiological study in rats from Group 1, 2 and 3. The epineural burying of sural nerve grafts with greater length and placed next to the neurorrhaphy’s site had a significantly better regeneration of the histological features than the smaller ones distant from neurorrhaphy.

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Comparison of nerve graft integration after segmentar resection versus epineural burying in crushed rat sciatic nerves

Cited by 3 publications

References 17 publications

Histopathological analysis of gangliosides use in peripheral nerve regeneration after axonotmesis in rats

Histopathological analysis of gangliosides use in peripheral nerve regeneration after axonotmesis in rats

Functional histopathological and morphometric study of the use of gangliosides in nerve regeneration in rats after axonotmesis

Partial epineural burying of nerve grafts with different sizes next to or distant from neurorrhaphy’s site: histological and electrophysiological studies in rat sciatic nerves

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