Aerial stem and leaf morphoanatomy of some species of Smilax

Martins, Aline Redondo; Bombo, Aline Bertolosi; Soares, Anielca Nascimento; Appezzato‐da‐Glória, Beatriz

doi:10.1590/s0102-695x2013005000043

Cited by 15 publications

(25 citation statements)

References 13 publications

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“…The cross section of the S. purhampuy leaf indicated that the abaxial and adaxial epidermis are uniseriate, with the existence of an easily perceptible cuticle. Morphoanatomic studies performed on the leaves of various Smilax species (S. brasiliensis, S. campestris, S. cisoides, S. fluminensis, S. goyazana, S. oblongifolia, and S. rufescens) revealed a nonstratified epidermis with thick cuticle (Martins et al, 2013b). These results correspond to those of previous studies and may be a distinctive anatomical characteristic of the genus.…”

Section: Microscopic Analysissupporting

confidence: 89%

“…In S. purhampuy a dorsiventral mesophyll was observed where the palisade parenchyma is toward the adaxial side and the spongy parenchyma was located toward the abaxial side, which supports the results of Martins & Appezzato-da Glória (2006) for S. polyantha and Martins et al (2013b) for S. brasiliensis, S. campestris, S. cissoids, S. goyazana, S. oblongifolia and S. rufescens. The presence of six vascular bundles of variable size at the level of the central vein of the leaf was unique and differed from other Smilax species (S. brasiliensis, S. cissoides, and S. fluminensis), which present three vascular bundles (Martins et al, 2013b).…”

Section: Microscopic Analysissupporting

confidence: 84%

“…Leaves are simple, and alternating with petioles that have tendrils; the primary venation are acrodomous. Thicker stems are rippled while aerial stems are generally aculeate (Martins et al, 2013b). In Ecuador, the genus is not well-recorded, although approximately ten species of this genus have been reported in the country according to Gaskin (1999).…”

Section: Introductionmentioning

confidence: 99%

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Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

Soledispa

Santos-Ordóñez

Miranda

et al. 2021

PeerJ

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Smilax plants are distributed in tropical, subtropical, and temperate regions in both hemispheres of the world. They are used extensively in traditional medicines in a number of countries. However, morphological and molecular barcodes analysis, which may assist in the taxonomic identification of species, are lacking in Ecuador. In order to evaluate the micromorphological characteristics of these plants, cross sections of Smilax purhampuy leaves were obtained manually. The rhizome powder, which is typically used in traditional medicines, was analyzed for micromorphological characteristics. All samples were clarified with 1% sodium hypochlorite. Tissues were colored with 1% safranin in water and were fixed with glycerinated gelatin. DNA was extracted from the leaves using a modified CTAB method for molecular barcode characterization and PCR was performed using primers to amplify the different loci including the plastid genome regions atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer; and the nuclear DNA sequence ITS2. A DNA sequence similarity search was performed using BLAST in the GenBank nr database and phylogenetic analysis was performed using the maximum likelihood method according to the best model identified by MEGAX using a bootstrap test with 1,000 replicates. Results showed that the micromorphological evaluation of a leaf cross section depicted a concave arrangement of the central vein, which was more pronounced in the lower section and had a slight protuberance. The micromorphological analysis of the rhizome powder allowed the visualization of a group of cells with variable sizes in the parenchyma and revealed thickened xylematic vessels associated with other elements of the vascular system. Specific amplicons were detected in DNA barcoding for all the barcodes tested except for the trnH–psbA spacer. BLAST analysis revealed that the Smilax species was predominant in all the samples for each barcode; therefore, the genus Smilax was confirmed through DNA barcode analysis. The barcode sequences psbK-psbI, atpF-atpH, and ITS2 had a better resolution at the species level in phylogenetic analysis than the other barcodes we tested.

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Section: Microscopic Analysissupporting

confidence: 89%

Section: Microscopic Analysissupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

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Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

Soledispa

Santos-Ordóñez

Miranda

et al. 2021

PeerJ

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“…Phytochemistry study to reveal bioactive components of Smilax species have been conducted almost in China (Xu et al 2005) and America (Rugna et al 1999), while the systematic study had been undertaken to investigate the phylogenetic relationship among East Asian species and American species which belonged to section Nemexia (Fu et al 2005). Taxonomic study to reveal diversity and to solve complexity of Smilacaceae have been conducted in America and the Caribbean Islands (Ferrufino-Acosta 2010; Denk et al 2015), Brazil (Martins et al 2013), China (Chen and Koyama 2000;Qi et al 2013a, b), Thailand (Moore et al 2010), Java (Backer and Bakhuizen v.d. Brink 1968;Ungson and Sastrapradja 1976), Borneo (Merrill 1921).…”

Section: B I O D I V E R S I T a S 20 (1): 379-387 January 2019 280mentioning

confidence: 99%

The diversity of Smilax (Smilacaceae) in Besiq-Bermai and Bontang Forests, East Kalimantan, Indonesia

Sofiah

Sulistyaningsih

2018

Biodiversitas

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Sofiah S, Sulistyaningsih LD. 2019. The diversity of Smilax (Smilacaceae) in Besiq-Bermai and Bontang Forests, East Kalimantan, Indonesia. Biodiversitas 20: 279-287. The genus Smilax has taxonomic complexity problems and spacious distribution. Taxonomic study to reveal the diversity of Smilax species had been done in some regions, such as America, China, Japan, Thailand, and Indonesia. However, there is lack of information of Smilax species diversity in Kalimantan especially in East Kalimantan which lies in Sundaland biogeographic. This study was carried out to explore and record the diversity of Smilax species including the ecological and environmental data in Besiq-Bermai and Bontang forests in East Kalimantan, Indonesia. This research conducted on February and August in 2012 and July-August 2015 using exploration methods. Purposive random sampling was used to do the botanical sampling. The principal component analysis (PCA) was performed to determine the relationships between environmental components and Smilax species occurrences. There were five species of Smilax which were housed in those forests in East Kalimantan, namely, Smilax leucophylla Blume, Smilax gigantea Merr., Smilax odoratissima Blume, Smilax zeylanica L., and Smilax modesta A.DC. Smilax leucophylla and Smilax zeylanica are the most widely used by the local people for medicine. The taxonomic description, distribution, use, and vernacular name were given. The environmental factors that contribute significantly to Smilax's growing environment are temperature and light intensity.

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“…Tricomas tectores unicelulares e pluricelulares localizados na estípula apical e nas nervuras primárias e secundárias em folhas de plantas dos três locais desse estudo indicam uma estratégia de economia hídrica da espécie. Os lipídios presentes nas paredes das células basais dos tricomas contribuem para reduzir a perda apoplástica de água (Barros e Soares, 2013). Dessa forma, a localização estratégica dos tricomas em P. viridis pode contribuir para impedir a perda de água pelas células do feixe vascular e evitar a dessecação da estípula apical, e consequentemente, protegendo a gema apical tanto da desidratação quanto de choques mecânicos e, ataque de predadores e patógenos.…”

Section: Discussionunclassified

Avaliação da variação morfológica, anatômica e fitoquímica de >i<Banisteriopsis caapi>/i< (Spruce ex Griseb.) C.v. Morton e >i<Psychotria viridis>/i< Ruiz & Pav em diferentes ambientes, teor de alcaloides e citotoxicidade do chá Ayahuasca

Miranda¹

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Avaliação da variação morfológica, anatômica e fitoquímica de Banisteriopsis caapi (Spruce ex Griseb.) C.v. Morton e Psychotria viridis Ruiz & Pav em diferentes ambientes, teor de alcaloides e citotoxicidade do chá Ayahuasca Ordilena Ferreira de Miranda Tese apresentada para obtenção do título de Doutora em Ciências. Área de concentração: Fisiologia e Bioquímica de Plantas Piracicaba 2021 Ordilena Ferreira de Miranda Bióloga Avaliação da variação morfológica, anatômica e fitoquímica de Banisteriopsis caapi (Spruce ex Griseb.) C.v. Morton e Psychotria viridis Ruiz & Pav em diferentes ambientes, teor de alcaloides e citotoxicidade do chá Ayahuasca versão revisada de acordo com a resolução CoPGr 6018 de 2011

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Aerial stem and leaf morphoanatomy of some species of Smilax

Cited by 15 publications

References 13 publications

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

Molecular barcode and morphological analysis of Smilax purhampuy Ruiz, Ecuador

The diversity of Smilax (Smilacaceae) in Besiq-Bermai and Bontang Forests, East Kalimantan, Indonesia

Avaliação da variação morfológica, anatômica e fitoquímica de >i<Banisteriopsis caapi>/i< (Spruce ex Griseb.) C.v. Morton e >i<Psychotria viridis>/i< Ruiz & Pav em diferentes ambientes, teor de alcaloides e citotoxicidade do chá Ayahuasca

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