Role of the NO/KATP pathway in the protective effect of a sulfated-polysaccharide fraction from the algae Hypnea musciformis against ethanol-induced gastric damage in mice

Damasceno, Samara; Rodrigues, Jocélia C.; Silva, Renan O.; Nicolau, Lucas A.D.; Chaves, Luciano S.; Freitas, Ana Lúcia Ponte; Souza, Marcellus H.; Barbosa, André Luiz R.; Medeiros, Jand Venes R.

doi:10.1590/s0102-695x2013005000003

Cited by 27 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our experimental data confirm that oral administration of NAP results in macroscopic and microscopic gastric injuries, as evidenced by edema, epithelial cell loss, inflammatory cell infiltration, and hemorrhage. However, pretreatment with CG elicited protection against NAP‐induced macroscopic and microscopic gastric damage; all parameters of inflammation measured were decreased, an observation consistent with earlier reports that demonstrate gastroprotective actions of other PLSs [Silva et al, ; Damasceno et al, ].…”

Section: Discussionsupporting

confidence: 89%

Gastroprotective Properties of Cashew Gum, a Complex Heteropolysaccharide of Anacardium occidentale, in Naproxen‐Induced Gastrointestinal Damage in Rats

Carvalho

Silva

et al. 2015

Drug Development Research

Self Cite

View full text Add to dashboard Cite

Long-term use nonsteroidal anti-inflammatory drug is associated with gastrointestinal (GI) lesion formation. The aim of this study was to investigate the protective activity of cashew gum (CG), a complex heteropolysaccharide extracted from Anacardium occidentale on naproxen (NAP)-induced GI damage. Male Wistar rats were pretreated with vehicle or CG (1, 3, 10, and 30 mg/kg, p.o.) twice daily for 2 days; after 1 h, NAP (80 mg/kg, p.o.) was administered. The rats were euthanized on the 2nd day of treatment, 4 h after NAP administration. Stomach lesions were measured using digital calipers. The medial small intestine was used for the evaluation of macroscopic lesion scores. Samples of the stomach and the intestine were used for histological evaluation, and assays for glutathione (GSH), malonyldialdehyde (MDA), and myeloperoxidase (MPO). Additional rats were used to measure gastric mucus and secretion. Pretreatment with CG reduced the macroscopic and microscopic damage induced by NAP. CG significantly attenuated NAP-induced alterations in MPO, GSH, and MDA levels. Furthermore, CG returned adherent mucus levels to normal values. These results suggest that CG has a protective effect against GI damage via mechanisms that involve the inhibition of inflammation and increasing the amount of adherent mucus in mucosa.

show abstract

Section: Discussionsupporting

confidence: 89%

Gastroprotective Properties of Cashew Gum, a Complex Heteropolysaccharide of Anacardium occidentale, in Naproxen‐Induced Gastrointestinal Damage in Rats

Carvalho

Silva

et al. 2015

Drug Development Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, there was the preservation the integrity of the structure of the epithelium, reduction of oedema of submucosa, and the inhibition of inflammatory cell infiltrate, as verified by histopathological analysis. This data corroborate previous studies on the gastroprotective effects of other SP extracted from marine algae in experimental models of ethanol-induced gastric damage [ 25 , 26 , 27 , 28 ].…”

Section: Discussionsupporting

confidence: 92%

Gastroprotective Effects of Sulphated Polysaccharides from the Alga Caulerpa mexicana Reducing Ethanol-Induced Gastric Damage

et al. 2018

View full text Add to dashboard Cite

The development of the gastric lesion is complex and the result of the imbalance between aggressive and protective factors, involving the generation of free radicals and disturbance in nitric oxide (NO) production. Sulphated polysaccharides (SP), from marine algae, are widely used in biotechnological and pharmaceutical areas. In this study, we evaluated the effects of SP from the green marine alga Caulerpa mexicana (Cm-SP) in ethanol-induced gastric damage models in mice. Cm-SP (2, 20, or 200 mg/kg), administered p.o., significantly reduced gastric damage, and these effects were inhibited through pretreatment with indomethacin. Cm-SP (200 mg/kg) prevented the ethanol-induced decline in glutathione and restored its normal level. Moreover, it was able to normalize the elevated thiobarbituric acid reactive substance levels. However, Cm-SP did not show any significant effects on NO2/NO3 level, when compared to the ethanol group. The pretreatment with L- NAME induced gastric mucosal damage and did not inhibit the gastroprotective effect of Cm-SP (200 mg/kg). In conclusion, the gastroprotective effects of Cm-SP in mice involve prostaglandins and reduction in the oxidative stress and are independent of NO.

show abstract

“…rubra [ 17 ], the involvement of PrLP in the antioxidant defense mechanisms was investigated through determination of glutathione (GSH) levels in the gastric mucosa. Animals from the negative control group (saline) displayed glutathione (GSH) levels within normal range, according to the published literature [ 55 ]. Animals pretreated with vehicle (positive control group) and subjected to intragastric absolute ethanol displayed significant mucosal GSH depletion when compared with the negative control group ( P < 0.05).…”

Section: Resultsmentioning

confidence: 99%

The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/K_ATPSignaling Pathway

Alencar

Pinheiro

Figueiredo³

et al. 2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Plumeria rubra (Apocynaceae) is frequently used in folk medicine for the treatment of gastrointestinal disorders, hepatitis, and tracheitis, among other infirmities. The aim of this study was to investigate the gastroprotective potential of a protein fraction isolated from the latex of Plumeria rubra (PrLP) against ethanol-induced gastric lesions and describe the underlying mechanisms. In a dose-dependent manner, the pretreatment with PrLP prevented ethanol-induced gastric lesions in mice after single intravenous administration. The gastroprotective mechanism of PrLP was associated with the involvement of prostaglandins and balance of oxidant/antioxidant factors. Secondarily, the NO/cGMP/KATP pathway and activation of capsaicin-sensitive primary afferents were also demonstrated as part of the mechanism. This study shows that proteins extracted from the latex of P. rubra prevent gastric lesions induced in experimental animals. Also, the results support the use of the plant in folk medicine.

show abstract

Role of the NO/KATP pathway in the protective effect of a sulfated-polysaccharide fraction from the algae Hypnea musciformis against ethanol-induced gastric damage in mice

Cited by 27 publications

References 49 publications

Gastroprotective Properties of Cashew Gum, a Complex Heteropolysaccharide of Anacardium occidentale, in Naproxen‐Induced Gastrointestinal Damage in Rats

Gastroprotective Properties of Cashew Gum, a Complex Heteropolysaccharide of Anacardium occidentale, in Naproxen‐Induced Gastrointestinal Damage in Rats

Gastroprotective Effects of Sulphated Polysaccharides from the Alga Caulerpa mexicana Reducing Ethanol-Induced Gastric Damage

The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/K_ATPSignaling Pathway

Contact Info

Product

Resources

About

Role of the NO/KATP pathway in the protective effect of a sulfated-polysaccharide fraction from the algae Hypnea musciformis against ethanol-induced gastric damage in mice

Cited by 27 publications

References 49 publications

Gastroprotective Properties of Cashew Gum, a Complex Heteropolysaccharide of Anacardium occidentale, in Naproxen‐Induced Gastrointestinal Damage in Rats

Gastroprotective Properties of Cashew Gum, a Complex Heteropolysaccharide of Anacardium occidentale, in Naproxen‐Induced Gastrointestinal Damage in Rats

Gastroprotective Effects of Sulphated Polysaccharides from the Alga Caulerpa mexicana Reducing Ethanol-Induced Gastric Damage

The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/KATPSignaling Pathway

Contact Info

Product

Resources

About

The Preventive Effect on Ethanol-Induced Gastric Lesions of the Medicinal PlantPlumeria rubra: Involvement of the Latex Proteins in the NO/cGMP/K_ATPSignaling Pathway