Attenuating effect of seeds of Adenanthera pavonina aqueous extract in neuropathic pain in streptozotocin-induced diabetic rats: an evidence of neuroprotective effects

Punchihewa

BMC Complement Altern Med

2016

161

BackgroundDiabetes has caused a major burden to the health sector in the developing countries and has shown an increasing trend among the urban population. It is estimated that most patients are with type II diabetes which could be easily treated with dietary changes, exercise, and medication. Sri Lanka carries a long history ayurvedic medicine where it uses the plant for treating many diseases. Therefore it is important to screen medicinal plants scientifically so they could be used safely and effectively in the traditional medical system and also be used for further investigations. Adenanthera pavonina is a plant used in the Ayurvedic medical system in Sri Lanka for treating many diseases including diabetics. We evaluated the anti-diabetic properties and the antioxidant properties of Adenanthera pavonina leaves.MethodsThe methanol extract of the leaves was sequentially extracted with petroleum ether and thereafter was partitioned between EtOAc, and water. The α-amylase inhibition assay was performed using the 3,5- dinitrosalicylic acid method. The antioxidant activities were measured using the DPPH free radical scavenging activity and the total phenolic content using Folin-Ciocalteu’s reagent. The cytotoxicity of the extract was evaluated using the Brine shrimp bioassay.ResultsThe IC50 values of α amylase inhibitory activity of MeOH, EtOAc, petroleum ether, and water were 16.16 ± 2.23, 59.93 ± 0.25, 145.49 ± 4.86 and 214.85 ± 9.72 μg/ml respectively and was similar to that of Acarbose (18.63 ± 1.21 (μg/ml). Antioxidant activities were also determined and the EtOAc fraction showed the highest total phenolic content (34. 62 ± 1.14 mg/g extract) and the highest DPPH scavenging activity with an IC50 of 249.92 ± 3.35 μg/ml.ConclusionThe leaf extracts of Adenanthera pavonina exhibit remarkable α-amylase inhibitory activity in the crude methanolic extract. Hence leaves of Adenanthera pavonina has a potential to be used as a regular green vegetable and also be investigated further in isolating pure compounds with anti-diabetic activity.

Section: Introductionmentioning

confidence: 99%

In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina

Punchihewa

BMC Complement Altern Med

2016

161

Evidence-Based Complementary and Alternative Medicine

“…The treated group showed an increased tail flick latency significantly when compared with pregabalin and reduced superoxide anion and total calcium levels which gave evidence of neuroprotective effects [145]. …”

Section: Resultsmentioning

confidence: 99%

“…Nigella sativa , Ocimum sanctum , Teucrium polium , Phyllanthus amarus, and Sesbania sesban also have been tested in single studies to ameliorate neuropathic pain [141–145]. …”

Section: Discussionmentioning

confidence: 99%

Can Medical Herbs Stimulate Regeneration or Neuroprotection and Treat Neuropathic Pain in Chemotherapy-Induced Peripheral Neuropathy?

Schröder

Beckmann

Franconi

et al. 2013

Chemotherapy-induced neuropathy (CIPN) has a relevant impact on the quality of life of cancer patients. There are no curative conventional treatments, so further options have to be investigated. We conducted a systematic review in English and Chinese language databases to illuminate the role of medical herbs. 26 relevant studies on 5 single herbs, one extract, one receptor-agonist, and 8 combinations of herbs were identified focusing on the single herbs Acorus calamus rhizoma, Cannabis sativa fructus, Chamomilla matricaria, Ginkgo biloba, Salvia officinalis, Sweet bee venom, Fritillaria cirrhosae bulbus, and the herbal combinations Bu Yang Huan Wu, modified Bu Yang Huan Wu plus Liuwei Di Huang, modified Chai Hu Long Gu Mu Li Wan, Geranii herba plus Aconiti lateralis praeparata radix , Niu Che Sen Qi Wan (Goshajinkigan), Gui Zhi Jia Shu Fu Tang (Keishikajutsubuto), Huang Qi Wu Wu Tang (Ogikeishigomotsuto), and Shao Yao Gan Cao Tang (Shakuyakukanzoto). The knowledge of mechanism of action is still limited, the quality of clinical trials needs further improvement, and studies have not yielded enough evidence to establish a standard practice, but a lot of promising substances have been identified. While CIPN has multiple mechanisms of neuronal degeneration, a combination of herbs or substances might deal with multiple targets for the aim of neuroprotection or neuroregeneration in CIPN.

“…The results showed that the pre-treatment with the seed extracts possess beneficial antiinflammatory effects by suppressed nitric oxide production and superoxide anion generation, cell death, and nuclear fragmentation by inhibiting the H 2 O 2 mediated generation of oxidative damage in rat peritoneal macrophages, suggesting that the extract has a cytoprotective property against intracellular peroxide production. Pandhare et al (2012b) evaluated the ameliorative effect of seeds of A. pavonina aqueous extract in attenuating neuropathic pain in streptozotocin-induced diabetic rats during twelve weeks of treatment. Diabetic rats orally received the test extract in 50, 100, or 200 mg/kg per day and pregabalin as a standard drug.…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

Advances in the research of Adenanthera pavonina: From traditional use to intellectual property

GeronÃ§o¹,

Melo²,

Barros³

et al. 2020

J. Med. Plants Res.

Adenanthera pavonina, an Asian native leguminous, is a well known highly used plant in traditional medicine. Such broad herbal medicine applications motivated several researchers to study its chemical composition, mainly of its leaves, seeds, and barks, besides the pharmacological effects aiming to demonstrate scientific evidence of its empirical uses. Most studies have been conducted with plant derivatives, and more recent studies have focused on isolated compounds. The phytochemical screening revealed various secondary metabolites with diverse biological effects, including flavonoids, alkaloids, saponins, tannins, steroids, triterpenoids, polyphenols, anthraquinones, coumarins, glycosides and polysaccharides. Experimental studies of this plant have shown numerous pharmacological activities such as antidiarrheal, anti-inflammatory, antinociceptive, antioxidant, antimicrobial, anticancer, and others. Advances in the studies of phytochemistry and biological activity on this plant species over the last years led to the increasing interest of researchers in the protection of inventions focusing on its cosmetic, pharmaceutical and food applicability. This review summarizes the tremendous therapeutic and technological potential of A. pavonina that should be explored, opening new perspectives for future researches and the development of new products.