Effects of the topical application of an ethyl acetate fraction from Vernonia scorpioides on excisional wounds infected with Staphylococcus aureus in rats

Abstract

Abstract:We investigated the healing process on excisional wounds infected with Staphylococcus aureus in rats, treated with 50 μL of ethyl acetate III from Vernonia scorpioides (Lam.) Pers., Asteraceae, rifamycin diethylamide B 25 mg, or saline. The lesions were measured daily and after seven days were surgically removed and histologically processed. The results indicate a favorable action of the EAIII, demonstrated by the increased wound contraction, smaller area of necrotic tissue, good development of granulation tissue, extensive extracellular matrix deposition and epithelial regeneration. This sub-fraction was phytochemically investigated in parallel studies, revealing the presence of sesquiterpene lactones (glaucolides and hirsutinolides) such as diacethylpiptocarphol and related hirsutinolides, flavonoids and cinnamic acid derivatives and also a new polyacetylene, which have been previously published. Results support the effectiveness of V. scorpioides antimicrobial activity in infected wound healing in rats.

A review on antimicrobial potential of species of the genus Vernonia (Asteraceae)

“…Rifamycin B diethylamide was used as positive control and saline as negative control. The results pointed to effective antimicrobial activity for wound healing in infected mice with increased wound contraction, smaller area of necrotic tissue, good development of granulation tissue, extensive array of extracellular matrix and epithelial regeneration (Kreuger et al, 2012). The ethanol extract of V. scorpioides showed antimicrobial activity against a strain of the yeast C. albicans and the bacterium S. aureus, with inhibition zones greater than 16 mm that were concentration dependent for grampositive cocci and greater than 15 mm for the tested yeast.…”