Abstract:Abstract:The chemical composition of the essential oil obtained from the fresh and dried leaves of Lippia gracillis Schauer, Verbenaceae, was analyzed by gas chromatography and gas chromatography/mass spectrometry (GC⁄MS). The yield of essential oil extracted from the dried leaves was significantly higher (p<0.05) when compared to the fresh leaves. Seventeen components were identified. The monoterpenes and sesquiterpene hydrocarbons with 96.26% (w/w) of the total oil obtained of fresh leaves and 86.99% (w/w) o… Show more
“…However, studies by Bitu et al (2012) showed that there are significant differences when comparing the chemical composition and antimicrobial activity of essential oils from fresh and dried rosemary leaves, where the biological activity can be modified by the leaf drying process.…”
Section: Data Relating To Medicinal Plants Soldmentioning
“…However, studies by Bitu et al (2012) showed that there are significant differences when comparing the chemical composition and antimicrobial activity of essential oils from fresh and dried rosemary leaves, where the biological activity can be modified by the leaf drying process.…”
Section: Data Relating To Medicinal Plants Soldmentioning
“…Results of MIC was considered positive for those wells with blue coloring indicating absence of visible microbial growth, and negative for those wells in red due the presence of viable cells 13,14 .…”
The main cause of increasing of infectious diseases cases is due to multidrugresistant microorganisms emergence, particularly Pseudomonas aeruginosa and Staphylococcus aureus, responsible for most of hospital-acquired infections and millions deaths related. Despite development of new antibiotics, control of these microorganisms is not always successful. Several plant extracts have demonstrated antimicrobial effects and may be used as an alternative therapy for these infections. Aiming to evaluate antibacterial activity of extracts from Eleutherine plicata (marupazinho), Geissospermum vellosii (pau-pereira) and Portulaca pilosa (amor crescido) against multidrugresistant bacteria, samples of Oxacillin-Resistant Staphylococcus aureus (ORSA) and multidrug-resistant P. aeruginosa (MDR P. aeruginosa) isolated from human clinical processes were tested. The antibacterial activity was determined by disk diffusion method and minimum inhibitory concentration (MIC) by microdilution method. Extracts and fractions were tested at concentrations of 500, 250, 125, 62.5, 31.2 and 16.2 µg/mL dissolved in DMSO 10%. E.
“…Several studies to identify antimicrobial activity have been conducted with plants that have high percentages of β-caryophyllene in their composition, presenting positive results for these extracts against several pathogens: Thymus kotschyanus [18], Spiranthera odoratissima [19], Lantana sp. [20], Vernonia remotiflorae V. brasiliana [21], Syzygium cumini [22], and Lippia gracillis [23], among others.Fig. 1Chemical structure of β-caryophyllene (4,11,11-trimethyl-8-methylene-bicyclo[7.2.0]undec-4-ene)…”
BackgroundPeriodontal disease is a highly prevalent illness that affects many dogs, reaching up to 85 % prevalence in individuals over the age of 4 years. Currently the drug of choice for combating the formation of dental plaque in these animals, the etiologic agent of the disease, is chlorhexidine, which has several side effects reported. Thus, surveys are conducted throughout the world in order to identify potential substitutes for antimicrobial therapy and prevention of periodontal disease. The objective of the work was to evaluate the antimicrobial activity of β-caryophyllene against bacteria from dog’s dental plaque in vitro and in vivo. The minimum inhibitory concentration was evaluated by agar microdilution assay, the induction or inhibition of bacterial adherence by sub-inhibitory concentrations in 96-well plates, and reduction of dental plaque formation in mongrel dogs subjected to topical solution with β-caryophyllene for 15 days.ResultsResults showed minimum inhibitory concentrations above 100 mg/mL for 25 % of the isolates, 100 mg/mL for 3 %, 50 mg/mL for 25 %, 25 mg/mL for 12 %, 12.5 mg/mL for 19 % and 6.25 mg/mL for 16 %. Bacterial adherences of three Enterococcus sp., one Streptococcus sp., one Haemophilus sp., one Aerococcus sp., one Bacillus sp. and one Lactococcus sp. isolates were inhibited by subinhibitory concentration. One Lactococcus sp., one Bacillus sp. and one Streptococcus sp. were stimulated to adhere by concentrations of 0.19, 1.56 and 0.78 mg/mL, respectively. In vivo assay showed reduction in dental plaque formation by β-caryophyllene, with final plaque coverage of 23.3 ± 2.6 % of the total area of the teeth, with significant difference compared with chlorhexidine group (37.5 ± 3.7 % - p < 0.05) and negative control group (65.5 ± 2.5 % - p < 0.001).ConclusionsThe results showed that β-caryophyllene has antimicrobial activity against the proliferation of dog’s dental plaque-forming bacteria representing a suitable alternative to the use of chlorhexidine in prophylaxis and treatment of periodontal disease of dogs.
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