Estudo de toxicologia clínica de um fitoterápico contendo Passiflora incarnata L., Crataegus oxyacantha L., Salix alba L. em voluntários saudáveis

Nascimento, Demétrius Fernandes do; Santana, Ana Paula M.; Leite, Ismenia O.; Viana, Fernando André C.; Leite, Ana Lourdes A. e Silva; Moraes, Renata A. de; Jamacaru, Francisco Vagnaldo Fechine; Bezerra, Fernando Antônio Frota; Moraes, Manoel Odoríco de; Moraes, Maria Elisabete Amaral de

doi:10.1590/s0102-695x2009000200014

Cited by 15 publications

(16 citation statements)

References 16 publications

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“…The histological results obtained for the control group of this study are similar to the ones of the literature -hepatocytes of the same size, with the presence of one or two central nuclei, displaying extensive anastomosed plaques, separated by the sinusoid capillaries; and acidophilic cytoplasm [21][22][23].…”

Section: Discussionsupporting

confidence: 78%

“…The main alterations detected were the initial stages of tissue disorganization and the presence of hepatocytes with more acidophilic cytoplasm. These alterations may be occurring in order to filter the blood containing foreign substances more efficiently and/or to allow the arrival of a larger amount of erythrocytes/leukocytes to participate in the metabolization of these substances [23].…”

Section: Discussionmentioning

confidence: 99%

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Hepatic and splenic cytotoxic evaluation after Crataegus oxyacantha fruit extract administration on mice

Santos¹,

Oliveira²,

Camargo‐Mathias³

et al. 2019

J Histol Histopathol

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The plant Crataegus oxyacantha L., Rosaceae, also known as white hawthorn or hawthorn, is originally native to Europe, North America and Asia, but can also be found in other continents and has been widely used due to its potential pharmacological effects, such as cardiotonic, anti-oxidant, hypolipidemic, antiinflammatory, sedative, among others. Considering the importance of C. oxyacantha as a natural alternative medicine, and the lack of studies involving its cellular toxicity, the objective of this study was to analyze the morpho-histological effects of C. oxyacantha fruit extract in the liver and spleen cells of mice supplemented with 50 (I), 100 (II) or200 mg/kg (III) of extract, in order to evaluate its cytotoxic effects. The liver of the individuals in group I showed no damage. Individuals in group II presented liver in the early stages of tissue and cytoplasmic disorganization. Those of group III suffered major histological alterations such as extensive cytoplasmic disorganization, appearance of vacuoles and increased sinusoidal capillaries, and large amounts of Kupffer cells. On the other hand, the spleen was not histologically modified after the treatments, maintaining its architecture as in the control group. Histochemical analyzes revealed alteration in the liver only, both in the amount of polysaccharides and lipids. It can be concluded that supplementation with C. oxyacantha fruit extract in higher doses leads to tissue disorganization in the liver of mice that indicates the need for caution with continuous and/or high doses use of this plant.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Hepatic and splenic cytotoxic evaluation after Crataegus oxyacantha fruit extract administration on mice

Santos¹,

Oliveira²,

Camargo‐Mathias³

et al. 2019

J Histol Histopathol

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“…However, few plants have been studied scientifically in order to evaluate their safe and efficacy (Calixto, 2005;Silveira et al, 2008;Tagliati et al, 2008;Nascimento et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic effects of infusions (tea) of Solidago microglossa DC. (Asteraceae) on the cell cycle of Allium cepa

Bagatini¹,

Fachinetto²,

Silva³

et al. 2009

Rev. bras. farmacogn.

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DC. (Asteraceae) no ciclo celular de Allium cepa". A espécie medicinal Solidago micrglossa DC., a qual é nativa da América do Sul, é usada como infusão (chá) para desordens estomacais na medicina popular. O objetivo desse estudo foi avaliar o potencial efeito citotóxico dessas infusões no ciclo celular de Allium cepa. Folhas in natura de três populações dessa espécie foram utilizadas no preparo das infusões em duas concentrações: 1,75 mg/mL e 14 mg/mL. Células de raízes de cebola (Allium cepa) foram usadas como sistema teste nos ensaios in vivo. As lâminas foram feitas através da técnica de esmagamento. As células foram analisadas em todos os ciclos celulares de A. cepa, totalizando 6000 células para cada grupo de bulbos. O índice mitótico (IM) foi calculado e a análise estatística foi realizada através do teste Qui-quadrado (χ 2 = 0,05). Os resultados mostraram que as infusões de S. microglossa na maior concentração (14 mg/mL) causou uma redução no IM comparado com os controles nas populações estudadas. Nas concentrações comumente usadas pelas pessoas houve um aumento significativo do IM em relação ao controle nas populações 1 e 3 e foi reduzido na população 2. Em conclusão, infusões de S. microglossa apresentam efeito antiproliferativo na maior concentração testada.Unitermos: Sistema teste vegetal, planta medicinal, citotóxico, Solidago microglossa, Asteraceae. ABSTRACT:The medicinal specie Solidago microglossa DC., which is native from South America, is used as infusion (tea) for stomach disorders in popular medicine. This study aimed to evaluate the potential cytotoxic effect of these infusions on the cell cycle of Allium cepa. In natura leaves of three populations of this species were used in preparing infusions at two concentrations: 1.75 mg/mL and 14 mg/mL. Onion (Allium cepa) root-tip cells were used as test system in in vivo assays. Slides were done through smashing technique. Cells were analyzed in all cell cycles of A. cepa, totalizing 6000 cells for each group of bulbs. The mitotic index (MI) was calculated, and the statistical analysis was carried out through the Chi-square test (χ 2 = 0.05). The results show that the infusions of S. microglossa at a highest concentration (14 mg/mL) caused a significant reduction in the MI compared with control in the studied populations. In concentrations commonly used by people there was a significant increase of MI in relation to controlled populations 1 and 3 and a decrease in population 2. In conclusion, infusions of S. microglossa presented antiproliferative effect at a highest concentration.

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“…12 It has been indicated for cardiovascular diseases such as hypertension, arrhythmia, and congestive heart failure, as well as for its antioxidant, antithrombosis, and anti-inflammatory activities. [13][14][15] Crataegus has cardioprotective effects, which are attributed to the cardiotonic properties of its flavonoid content and to oligomeric procyanidins, whose antioxidant effect can reduce myocardial oxidative stress after reperfusion and inhibit myocardial cell apoptosis. 13 The administration of this drug dilates peripheral vessels and relaxes coronary arteries, improves blood flow to the heart, and increases relaxation time, aiding heart disease and alleviating symptoms in early heart failure.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Efficacy of Crataegus oxyacantha in Dogs with Early-Stage Heart Failure

2020

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Background Myxomatous mitral valve disease (MMVD) is the most common cardiopathy in middle-aged dogs. When the dog is asymptomatic and has an enlarged left atrium, treatment is beneficial; however, some allopathic drugs are very costly and may produce side effects. To extend the duration of this asymptomatic phase, complementary therapies such as herbal medicine and homeopathy are available. Although herbal therapy with extract of Crataegus oxyacantha is beneficial, there is a risk of adverse reactions—unlike with homeopathy, where the risk is minimized with the administration of ultra-diluted doses. Objective This study evaluated the efficacy of Crataegus oxyacantha, as mother tincture (MT) and in 6 cH homeopathic formulation, in treating the initial phase of heart failure due to MMVD in a veterinary clinic setting. Methods A total of 30 dogs with MMVD, 7 years or older and weighing up to 10 kg, were randomized into three groups as follows: Crataegus 6 cH, Crataegus MT, and hydroalcoholic solution (placebo). Animals were evaluated through echocardiography parameters, laboratory blood tests, and systolic blood pressure (SBP) measurements at 30, 60, 90, and 120 days after initiation of therapy, for statistical analysis and monitoring of the blinded study. Results Patients who received Crataegus 6 cH showed a reduction in SBP 60 days after treatment, while those receiving Crataegus MT exhibited a reduction 90 days after the therapy was initiated. There was a significant linear regression when evaluating the effect of treatment with Crataegus 6 cH on SBP measurements over the evaluation intervals (linear equation: SBP = 176.57 mm Hg – 0.21x, where x represents days of treatment). There was an increase in both fractional shortening and isovolumetric relaxation time for those patients receiving the homeopathic formulation. Conclusions Therapy with Crataegus was beneficial for hypertensive and cardiopathic dogs with MMVD, extending the duration of the asymptomatic phase. The reduction in SBP occurred more swiftly in the 6 cH group than in the MT-treated dogs.

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Estudo de toxicologia clínica de um fitoterápico contendo Passiflora incarnata L., Crataegus oxyacantha L., Salix alba L. em voluntários saudáveis

Cited by 15 publications

References 16 publications

Hepatic and splenic cytotoxic evaluation after Crataegus oxyacantha fruit extract administration on mice

Hepatic and splenic cytotoxic evaluation after Crataegus oxyacantha fruit extract administration on mice

Cytotoxic effects of infusions (tea) of Solidago microglossa DC. (Asteraceae) on the cell cycle of Allium cepa

Evaluation of the Efficacy of Crataegus oxyacantha in Dogs with Early-Stage Heart Failure

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