Evaluation of the molluscicidal and Schistosoma mansoni cercariae activity of Croton floribundus extracts and kaurenoic acid

Abstract

RESUMO: "Avaliação das atividades moluscicida e cercaricida (Schistosoma mansoni) dos extratos de Croton floribundus e do ácido caurenóico". Extratos de Croton fluribundus (Euphorbiaceae), ácido caurenóico e dois derivados do ácido caurenóico foram avaliados como moluscicida, cercaricida e também foi verificada a letalidade destas amostras frente a larvas de Artemia salina Leach. Nestes ensaios foram observadas significantes atividades moluscicida e cercaricida associadas a uma reduzida toxicidade frente ao camarão de água salgada. Unitermos: Biomphalaria glabrata, Croton floribundus, Artemia salina, ácido caurenóico, Schistosoma mansoni, cercárias.ABSTRACT: Lethality of the extracts of Croton floribundus (Euphorbiaceae), a medicinal plant from south Brazil, and of the kaurenoic acid, an isolated compound, and two of its derivatives against adult Biomphalaria glabrata snails, Schistosoma mansoni cercariae and Artemia salina Leach. brine shrimp larvae are reported. Both extracts and the isolated compound showed significant molluscicidal and cercaricidal activities and reduced toxicity in brine shrimp assays.

Biological screening of extracts from leaf and stem bark of Croton floribundus Spreng. (Euphorbiaceae)

“…The molluscicidal activity was not observed for both extracts tested on Biomphalaria glabrata snails in the evaluated concentrations (200, 100 and 50 µg/ml). Medina et al (2009) reported significant molluscicidal and cercaricidal activities against adult Biomphalaria glabrata snails and Schistosoma mansoni cercariae for alcoholic extracts of leaves and barks of C. floribundus and the isolated compound, kaurenoic acid. The lack of activity observed in our study can be related to the non-production of active metabolites in the studied plant, since the production of secondary metabolites in plants can be influenced by several factors like seasonality, type of soil, weather, rainfall, radiation or any other condition (Al-Zanbagi et al, 2000).…”

“…The fruits are considered tonic. According Medina et al (2009), the alcoholic extracts of leaves and barks and the isolated compound, kaurenoic acid, showed significant molluscicidal and cercaricidal activities against adult Biomphalaria glabrata snails and Schistosoma mansoni cercariae, respectively. In other work, the ent-kaur-16-en-6α,19-diol isolated from hexane extract of root bark showed moderate effect against three cancer cell lines: MDA-MB-435 (melanoma), HCT-8 (colorectal adenocarcinoma) and HCT-116 (colorectal adenocarcinoma) (Uchoa et al, 2013).…”

Molluscicidal and cercaricidal activities of curcumin on Biomphalaria glabrata and Schistosoma mansoni cercariae

“…A previous study with CUR also demonstrated high activity on S japonicum cercariae, with 100% cercariae mortality at a concentration of 8 μg mL −1 after 30 min . Other compounds, such as kaurenoic acid isolated from Croton floribundus , have also demonstrated the cercaricidal effect on S. mansoni cercariae, with 100% cercariae mortality at a concentration of 100 μg mL −1 after 1 h of exposure . Although PZQ is not used to eliminate snail breeding cercariae, this compound was used during the present study as positive control.…”

“…30 Other compounds, such as kaurenoic acid isolated from Croton floribundus, have also demonstrated the cercaricidal effect on S. mansoni cercariae, with 100% cercariae mortality at a concentration of 100 μg mL −1 after 1 h of exposure. 48 Although PZQ is not used to eliminate snail breeding cercariae, this compound was used during the present study as positive control. In agreement with other reports, PZQ causes 100% mortality of S. mansoni and S. japonicum cercariae at concentrations of 1, 8 and 12.5 μg mL −1 between 0.30 and 3 h. 30,36,49 CUR toxicity related to other environment organisms was not evaluated in this study; however, CUR showed no toxic effect and systemic abnormalities in a higher dose (1.5 mg) against Labeo rohita fish.…”

From Bench to Bedside: Natural Products and Analogs for the Treatment of Neglected Tropical Diseases (NTDs)

“…glabrata snails at concentrations of 50 ppm [263]. glabrata adult snails [269]. glabrata and their eggs [264,265].…”