Atividade antimicrobiana e antiaderente in vitro do extrato de Rosmarinus officinalis Linn. sobre bactérias orais planctônicas

Abstract

"In vitro antimicrobial activity and antiadherence of Rosmarinus offi cinalis Linn. against oral planktonic bacteria". In this study was investigated the antimicrobial activity and in vitro adherence inhibition of a hydro alcoholic Rosmarinus offi cinalis Linn. (alecrim) on standard strains of Streptococcus mitis ATCC 98811, Streptococcus sanguinis ATCC 10556, Streptococcus mutans ATCC 25175, Streptococcus sobrinus ATCC 27609 and Lactobacillus casei ATCC 7469 extract. The test was carried out by inundation tecniques in Petri dishes to determine the Minimum Inhibitory Concentration (MIC) and inclined tubes techniques the Minimum Inhibitory Concentration of Adherence to glass (MICA) at the presence of 5% sucrose. Tests with gluconate of chlorexidine 0.12% were performed as controls. MICs of the Rosmarinus offi cinalis extract dilutions (mg/mL) against S. sanguinis ATCC 10556, S. mutans ATCC 25175, S. sobrinus ATCC 27609 and L. casei ATCC 7469 were 1:1, 1:4, 1:1 e 1:4, respectively. The extract from alecrim inhibited all the standard strains growth tested, except for S. mitis ATCC 98811. MICAs of the Rosmarinus offi cinalis extract dilutions (mg/mL) against S. mitis ATCC 98811, S. mutans ATCC 25175 e S. sobrinus ATCC were 1:8, 1:16 e 1:8, respectively. The results suggest that there is a possibility of the alecrim use as an oral antimicrobial. Nevertheless, study models which could reproduce situations similar to those seen in bucal caries are necessary for the antimicrobial agents evaluation in the treatment and biofi lm dependant oral infections prevention.

Isolation, Quantification, and Identification of Rosmarinic Acid, Gas Chromatography-Mass Spectrometry Analysis of Essential Oil, Cytotoxic Effect, and Antimicrobial Investigation of Rosmarinus Officinalis Leaves.

“…Hence, the use of traditionalalternative medicine as a source to treat infectious diseases has been accomplished since the origin of humankind [18]. Available information indicates that hydroalcoholic extract of rosemary was assayed against different strains of bacteria [19,20].…”

Dentifrice Containing Extract of Rosmarinus officinalis Linn.: An Antimicrobial Evaluation

“…The essential oil of rosemary has antibacterial, antioxidative and antifungal properties (8). Silva et al (9) found that the hydroalcoholic extract of Rosmarinus officinalis was effective against oral plankton bacteria (S. mitis, S. sanguinis, S. mutans, S. sobrinus and L. casei), involved in the formation of oral biofilm. The scope of phytotherapy using medicinal plants is vast, involving diverse purposes, including use in oral healthcare (10).…”

Plantas e constituintes químicos empregados em Odontologia: revisão de estudos etnofarmacológicos e de avaliação da atividade antimicrobiana in vitro em patógenos orais

“…No entanto, as altas taxas de S. aureus em pacientes com sintomas da que variam de dor, ardor, eritema e edema da mucosa oral, sugerem que é necessário abordar a possibilidade deste agente desempenhar um papel em doenças da mucosa oral. Isolados de S. aureus são capazes de produzir grande variedade de exotoxinas identificadas em isolados orais (Smith et al, 2003).…”