Terpenóides e avaliação do potencial antimalárico, larvicida, anti-radicalar e anticolinesterásico de Pouteria venosa (Sapotaceae)

Abstract

RESUMO:O presente trabalho descreve o isolamento de quatro triterpenos (taraxerol, ácido ursólico, ácido 3β,19α,23-triidroxiurs-12-en-28-óico e ácido 2α,3α,19α,23-tetraidroxiurs-12-en-28-óico) e um fi toesteróide (espinasterol), bem como a avaliação do potencial antimalárico (cepa NK-65 do Plasmodium berghei), larvicida (larvas do 4º instar do Aedes aegypti), anti-radicalar (2,2-difenil-1-picril-hidrazila, DPPH) e anticolinesterásico de extratos das folhas, cascas do caule e caule de Pouteria venosa (Mart.) Baehni. Todos os compostos isolados estão sendo descritos pela primeira vez nesta espécie e foram identifi cados com base na análise de dados espectrais (IV e RMN, incluindo APT e DEPT), bem como pela comparação com dados descritos na literatura.Unitermos: Pouteria venosa, terpenóides, anticolinesterase, anti-radicalar, malária, larvicida.ABSTRACT: "Terpenoids and evaluation of the antimalarial, larvicidal, anti-radicalar and anticholinesterase potential of Pouteria venosa (Sapotaceae)". This work describes the isolation of four triterpenes (taraxerol, ursolic acid, 3β,19α,23-trihydroxyurs-12-en-28-oic acid and 2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid) and a phytosteroid (spinasterol), as well as a preliminary evaluation of antimalarial (NK-65 strains of Plasmodium berghei), larvicidal (4 th instar of Aedes aegypti), anti-radicalar (2,2-diphenyl-1-pycryl-hydrazyl, DPPH) and anticholinesterase activities of Pouteria venosa (Mart.) Baehni extracts from leaves, stem barks and stems. All isolated compounds are being described for the fi rst time in this species and were identifi ed on basis of the spectral data (IR and NMR, including APT, DEPT), as well as by comparison with literature data

Genus Pouteria: chemistry and biological activity

“…This activity was also noted at ethanol extract and some fractions from leaves, stem and stem bark of P. venosa (Montenegro et al, 2006), in the ethyl acetate fraction of methanol extracts from P. sapota and from P. viridis fruits (Ma et al, 2004;Mahattanatawee et al, 2006), and also in the methanol and acetone extracts from P. campechiana fruit and P. sapota juice (Franco, 2006).…”

“…From ethanol extract of stems were isolated miriantic acid (19) and 19α,23-dihydroxyursolic acid (19a) (Montenegro et al, 2006).…”

“…Several Pouteria species have been used in folk medicine to treat fever, inflammation, skin eruptions, ulcers, diabetes (Ma et al, 2004;Montenegro et al, 2006) diarrhea (Perfeito et al, 2005), nausea, vomiting, back pain, and to promote lactation on milk feeding mothers (Manosroi et al, 2006). However, there is a lack of scientific evidence for most of these claimed biological activities.…”

“…The ethanol extract from P. venosa leaves (250 mg/mL), as well as the hydroethanol fraction (500 mg/mL), presented antimalarial (against Plasmodium berghei) activity in vivo reducing the induced infection in rats (Montenegro et al, 2006).…”

Triterpenos de Guettarda grazielae M: R.V. Barbosa (Rubiaceae)

“…Os compostos conhecidos como acetato de a-amirina (2) (Seo et al, 1975;Bandeira et al, 2007;Zanon et al, 2008), cicloartenona (5) (Davies et al, 1992), sitostenona (6) (Wehrli & Nishida, 1979; e ácido ursólico (7) (Connolly & Hill, 1991;Mahato & Kundu, 1994;Montenegro et al, 2006) foram identificados através da comparação dos dados de RMN obtidos com os da literatura.…”