Extratos metanólico e acetato de etila de Solanum megalonyx Sendtn. (Solanaceae) apresentam atividade espasmolítica em íleo isolado de cobaia: um estudo comparativo

Abstract

RESUMO:Solanum megalonyx Sendtn. (Solanaceae) é conhecida popularmente por "jurubeba" no Nordeste do Brasil e se apresenta na forma de arbusto. Várias espécies de Solanum apresentam efeito espasmolítico em órgãos isolados. Assim, objetivou-se investigar e comparar o efeito dos extratos metanólico (SM-MeOH) e acetato de etila (SM-AcOEt), obtidos das partes aéreas de S. megalonyx, em íleo isolado de cobaia. SM-MeOH e SM-AcOEt antagonizaram (n = 5) as contrações fásicas induzidas por 1 μM de acetilcolina (logCI 50 = 3,2 ± 0,1 e 1,8 ± 0,6 μg/mL, respectivamente) ou de histamina (logCI 50 = 2,8 ± 0,5 e 1,7 ± 0,3 μg/mL, respectivamente). SM-MeOH e SM-AcOEt também relaxaram (n = 5) o íleo pré-contraído por 40 mM de KCl (logCE 50 = 1,9 ± 0,09 e 1,9 ± 0,1 μg/mL, respectivamente), por 1 μM de histamina (logCE 50 = 1,9 ± 0,07 e 1,7 ± 0,08 μg/mL, respectivamente) ou de acetilcolina (logCE 50 = 1,9 ± 0,02 e 1,7 ± 0,09 μg/mL, respectivamente) de maneira dependente de concentração e equipotente. Demonstra-se pela primeira vez que S. megalonyx apresenta efeito espasmolítico não seletivo em íleo isolado de cobaia, sugerindo que os extratos podem estar agindo em um passo comum da via de sinalização dos agentes contráteis testados.Unitermos: Solanum megalonyx, Solanaceae, íleo de cobaia, atividade espasmolítica.ABSTRACT: "Methanol and ethyl acetate extracts from Solanum megalonyx Sendtn. (Solanaceae) present spasmolytic activity in guinea-pig ileum: a comparative study". Solanum megalonyx Sendtn. (Solanaceae) is known popularly as "jurubeba" in Northeastern Brazil where it can be found as a shrub. Several species of Solanum present spasmolytic effect in several tissues, thus this study was aimed to investigate and compare the effect of the methanol extract (SMMeOH) and ethyl acetate extract (SM-AcOEt), obtained from aerial parts of Solanum megalonyx Sendtn., in guinea-pig ileum. In this work, both SM-MeOH and SM-AcOEt antagonized the phasic contraction induced by acetylcholine 1 μM (logIC 50 = 3.2 ± 0.1 and 1.8 ± 0.6 μg/mL) and histamine 1 μM (logIC 50 = 2.8 ± 0.5 and 1.7 ± 0.3 μg/mL, respectively) (n = 5), without statistical differences between these values. In another set of experiments, SM-MeOH and SM-AcOEt also relaxed the isolated guinea-pig ileum pre-contracted by KCl 40 mM (logEC 50 = 1.9 ± 0.09 and 1.9 ± 0.1 μg/mL, respectively), histamine 1 μM (logEC 50 = 1.9 ± 0.07 μg/mL and 1.7 ± 0.08 μg/mL, respectively) or acetylcholine (logEC 50 = 1.9 ± 0.02 μg/mL and 1.7 ± 0.09 μg/mL, respectively) (n = 5) in a concentration-dependent and equipotent manner. This study demonstrates for the fi rst time that aerial parts of S. megalonyx present a non-selective spasmolytic effect in guinea-pig ileum, suggesting that the extracts could be acting in a common step of the pathway signaling that leads to contraction induced by the contractile agents tested.

Effects of a tomato (Solanum lycopersicum) extract on the labeling of blood constituents with technetium-99m

“…Some epidemiological and experimental data suggest an inverse relation between intake of tomato and risk of cancer at various anatomical sites, especially prostate and colon (Thomson and Ali, 2003;Etminan et al, 2004;Canene-Adams et al, 2007). Besides its potential role in preventing and treating cancer, tomato intake has also been studied for use in the prevention of atherosclerosis (Rissanen et al, 2002;Frederiksen et al, 2007), reduction of asthma symptoms (Neuman et al, 2000;Wood et al, 2004) as hypolipidemic effect (Gonçalves et al, 2006a,b), inhibitor of the angiotensin converting enzyme (Barbosa-fi lho et al, 2006), present spasmolytic activity (Oliveira et al, 2006), hypoglycemic activity (Barbosa-Filho et al, 2005) and decrease of DNA strand breakages of cells of the immune system (Riso et al, 1999;Porrini et al, 2005;Riso et al, 2006). The importance of these plants has promoted their inclusion in Brazilian Pharmacopoeia (Brandão et al, 2006;.…”

Toxicological, Antidiarrheal and Spasmolytic Activities of Solanum paniculatum

“…[11] have shown antidiarrheal activity in mice. Additionally, there are evidences that some species exhibit spasmolytic activity on the guinea pig ileum, such as Solanum megalonyx Sendtner, S. asterophorum [12,13], Solanum jabrense Agra & Nee [14], and Solanum agrarium Sendtner [15]. Solanum paniculatum L. is a shrubby species popularly known as "jurubeba-verdadeira" [16].…”