Influence of surgical technique in the peritoneal carcinomatosis surgical wound implant: experimental model in mice

Abstract

BackgroundThe number of malignancies increased alarmingly. Surgery constitutes one of the most efficient therapeutic modalities for the treatment of solid tumors. The neoplastic implant in surgical wound is a complication whose percentage of occurrence reported in the literature is variable, but sets with high morbidity and therapeutic difficulties. Protecting the wound is one of the recommended principles of oncologic surgery.AimTo evaluate the influence of wound protection in the development of tumor implantation.MethodsSarcoma 180 tumor cells were used, with intraperitoneal inoculation in Swiss mice. After the establishment of neoplastic ascites, animals were randomized into two groups of 10, each group consisting of five males and five females. In both groups, laparotomy and manipulation of intra-abdominal organs was performed. In a group laparotomy was performed using the protection of the abdominal wound and the other group without it. On the 9th postoperative day macroscopic evaluation of the operative scar was performed, which was later removed for microscopic evaluation.ResultsThere was microscopic infiltration of tumor cells in the wound of all animals. However, the group that held the protection, infiltration was less intense when compared to the group without it. The infiltration was also more severe in females than in males of the same group.ConclusionTumor infiltration into the wound was more intense in the group in which the protection of the surgical site was not performed, and in females when compared to males of the same group.

Comparison of implantation sites for the development of peritoneal metastasis in a colorectal cancer mouse model using non-invasive bioluminescence imaging

“…Our study confirmed that scratching created an environment conducive for the development of PMc in the mouse model (i.e. inflammation, growth factor release and angiogenesis for the wound-healing process) [26, 27]. This model may be used to test and validate palliative strategies, which have not yet been established, by evaluating new palliative drugs or surgical methods for extensive PMc.…”

Treatment of Facial Nevi with Ideal Design and Splitting Technique

“…2. The dissection should be performed in deep subcutaneous tissue, but not the super cial layer (13)(14)(15). This group did not nd any seeding of nevus cells due to surgery.…”