Correlação da infiltração das células Natural Killer (NK) CD 57+ no prognóstico do adenocarcinoma gástrico

Abstract

DESCRITORES ABCDDV/854ABSTRACT -Background -To evaluate the concentration of Natural Killer cells (NK) cells in adenocarcinoma of the stomach, and its correlation with prognostic factors and survival. Methods -Seventy-two patients with gastric adenocarcinoma who underwent gastric resection surgery and D2 lymphadenectomy in the period 1997-2007 were analyzed. The concentration of NK cells was evaluated by immunohistochemistry technique with the reagent CD57. Patients were divided into two groups: high concentration (n = 32) (more than 15 cells per 10 high power field) and low concentration (less or equal than 15 cells per 10 high power field). These two groups were compared with several prognostic factors such as: gender, age, tumor location, tumor differentiation, Lauren classification, stage, lymph nodes involvement, distant metastases and survival. The Kaplan-Meier curve was applied to evaluate survival and multivariate analysis of prognostic factors for evaluation.Results -There was no relationship of NK cells with the several variables studied, except to the tumor stage (p<0.02) with high concentration in more advanced stages. Survival was better (p = 0.0025) in the group of high concentration of NK. The survival analysis in advanced stage shown that the group with high concentration had higher survival (p<0.0001). In multivariate analysis, the concentration of NK cells was an independent prognostic factor (p = 0.0027, Hazard Ratio = 0343). Conclusions -The concentration of NK cells did not differ among the prognostic variables, with the exception of the staging. Patients with high concentration of NK cells showed a higher survival rate when compared to the low concentration, especially in the advanced stage.

Prognostic significance of tumor immune microenvironment and immunotherapy: Novel insights and future perspectives in gastric cancer

“…The data show that NK cells exhibit cytotoxic activity against allogeneic and autologous cancer cell lines, including gastric cancer cells lines[ 147 ], and could prevent tumor metastatic spreading[ 148 , 149 ]; additionally, intra-tumor infiltration of NK cells is associated with a longer survival in neoplastic patients[ 150 ]. Patients with advanced gastric adenocarcinoma having a high density of NK cells demonstrated a prolonged survival rate vs those with the low density NK[ 151 ]. The number of apoptotic NK cells (Fas + NK cells) is significantly higher in gastric neoplasia patients vs normal controls and is correlated to the tumor progression[ 152 ].…”

Chemokine Receptor CXCR3 Correlates with Decreased M2 Macrophage Infiltration and Favorable Prognosis in Gastric Cancer

“…The prognosis of the GC is significantly correlated with the tumor-associated immune cells in tumor immune microenvironment [2]. Tumor-associated immune cells such as natural killer (NK) cells, dendritic (DC) cells, helper T1 (Th1) cells, and cytotoxic CD8 cells in the tumor microenvironment are generally correlated with favorable outcome [3–6]. In contrast, infiltration of B cells, regulatory T (Tregs) cells, and Th2 and Th17 cells promotes tumor development and correlates with a poor prognosis [7–9].…”

Genotype B of Killer Cell Immunoglobulin-Like Receptor is Related with Gastric Cancer Lesions

“…It was recently reported that tumor-infiltrating NK cells were decreased in human GC; moreover, the production of IFNγ and TNFα by these cells was impaired by tumor-associated monocytes/macrophages via TGFβ1 36 . In contrast, patients with GC had a better survival when they presented higher concentrations of NK cells, an effect that was more evident in advanced stage cases 37 . Our work associates the risk to develop GC with the B KIR genotypes and the gene cluster included within the telomeric part.…”