Utilização de método sorológico como ferramenta diagnóstica para implementação da vigilância e controle da esquistossomose no Município de Holambra, São Paulo, Brasil

Gargioni, Cybele; Silva, Rita Maria da; Thomé, Célia Maria; Quadros, Celma Maria da Silva; Kanamura, Hermínia Yohko

doi:10.1590/s0102-311x2008000200016

Cited by 7 publications

(5 citation statements)

References 13 publications

(11 reference statements)

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“…Other studies have demonstrated this relationship between the number of slides read and the positivity rate of human cases of schistosomiasis 5 . Thus, these data further reinforce the need for a diagnostic measure that can contribute and increase the efficiency of local actions of schistosomiasis control programs, since the guidelines of the Ministry of Health recommend the collection of a single sample of fecal material, followed by the reading of 2 slides in the areas endemic for the disease 29 .…”

Section: Discussionmentioning

confidence: 60%

New epidemiological profile of schistosomiasis from an area of low prevalence in Brazil

Santos

Bezerra

Cirilo

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Schistosomiasis, caused by infection from Schistosoma mansoni, is a disease that represents an important public health problem for Brazil, especially for states in the Northeast region. Thus, the aim of this study is to present a new epidemiological profile for the disease in a municipality with low prevalence in the state of Alagoas, Brazil. Methods: A cross-sectional study was conducted through a coproparasitological and malacological survey. A structured questionnaire was applied to the study participants to survey possible risk factors and a spatial analysis (kernel density) was used to measure the risk of infection. Results: Of the 347 participants, 106 (30.5%) were infected by Schistosoma mansoni, most of them from the urban area of the municipality (68.9%; 73/106). A 3-fold risk of infection was found for individuals living in the urban area and a risk of 2.15 times for self-declared farmers. Biomphalaria glabrata and B. straminea were the species found in the municipality, but no animals were diagnosed as infected by the parasite. Spatial analysis showed a random distribution of vectors and human cases of the disease, and the formation of two clusters of human cases in the urban area was seen. Conclusions: A new epidemiological profile for schistosomiasis from S. mansoni infection was presented in a municipality of low endemicity: a high proportion of positive individuals in the urban area; presence of snails without positive diagnosis for S. mansoni infection; random distribution of vectors and human cases; and absence of association between classical risk factors and human infection.

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Section: Discussionmentioning

confidence: 60%

New epidemiological profile of schistosomiasis from an area of low prevalence in Brazil

Santos

Bezerra

Cirilo

et al. 2020

Rev. Soc. Bras. Med. Trop.

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“…Since 1976, parasitological testing of feces has been used by state schistosomiasis control programs as a criterion for selective treatment with praziquantel 14 . The worldwide methodological strategy currently applied for the diagnosis of mansoni schistosomiasis, using the KK method with one sample and one slide to determine the infection rate, does not have the sensitivity required for such diagnosis and particularly in areas of low endemicity underestimates the prevalence of this parasitosis 15 .…”

Section: Discussionmentioning

confidence: 99%

Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area

Carneiro

Pinheiro

Oliveira

et al. 2012

Rev. Soc. Bras. Med. Trop.

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Introduction:The laboratory diagnosis of schistosomiasis is based mainly on the detection of parasite eggs in stool samples through the Kato-Katz (KK) technique, reading one slide by test. However, a widely known limitation of parasitological methods is reduced sensitivity, particularly in low endemic areas. Methods: To increase sensitivity, we conducted further slide readings from the same stool sample using the parasitological method associated with a serological test. We used the KK method (three slides) and the IgG anti-Schistosoma mansonienzyme-linked immunosorbent assay (ELISA) technique to diagnose schistosomiasis in low endemic areas in the Brazilian State of Ceará. Fecal samples and sera from 250 individuals were analyzed. Results: Sixteen percent and 47.2% of samples were positive in parasitological tests and serological tests, respectively. Parasitological methods showed that 32 (80%) individuals tested positive on the first slide, 6 (15%) on the second slide, and 2 (5%) on the third. The performance of the ELISA test in the diagnosis, using the KK method as diagnostic reference, showed a negative predictive value of 100%, with specificity and positive predictive values of 62.8% and 33.9%, respectively. Conclusions: In this study, the increase from one to three slides analyzed per sample using the KK technique was shown to be a useful procedure for increasing the diagnostic sensitivity of this technique.

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“…This IFT was applied in different areas of the State of São Paulo, Brazil, with no schistosomiasis and high prevalence for other helminth infections, such as ascaridiasis, trichuriasis, and enterobiasis [27]. There are various studies that assess the potential of different immunodiagnostic methods in populations [28–31], and, even when applied in low endemicity areas, these immunodiagnostic assays show a good relation between sensitivity and positivity ratio but still cannot discriminate the active infection.…”

Section: Immunodiagnostic Methods For Antibodies Detectionmentioning

confidence: 99%

Immunodiagnostic Methods: What Is Their Role in Areas of Low Endemicity?

Grenfell

Silva-Moraes

Taboada

et al. 2012

The Scientific World Journal

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Worldwide Schistosomiasis mansoni continues to be a serious public health problem. Over the past decades, control programmes have made remarkable progress in reducing S. mansoni infections to a relatively low level in Brazil and African countries. Endemic regions are currently circumscribed in certain core areas where reinfection and repeated chemotherapy are frequent and, consequently, are related to residents with low parasite load. At present, diagnosis is predominately a key step for final disease control although low endemicity area residents are hardly detected by most of the available assays. In this paper, we review the current status and efforts made aiming at the improvement of diagnostic tools for S. mansoni in low endemicity infections. The establishment of diagnostic assays—simple, affordable, sensitive, and specific for field diagnosis of S. mansoni—is essential and should be given high priority.

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Utilização de método sorológico como ferramenta diagnóstica para implementação da vigilância e controle da esquistossomose no Município de Holambra, São Paulo, Brasil

Cited by 7 publications

References 13 publications

New epidemiological profile of schistosomiasis from an area of low prevalence in Brazil

New epidemiological profile of schistosomiasis from an area of low prevalence in Brazil

Increased detection of schistosomiasis with Kato-Katz and SWAP-IgG-ELISA in a Northeastern Brazil low-intensity transmission area

Immunodiagnostic Methods: What Is Their Role in Areas of Low Endemicity?

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