Resistência antimicrobiana de coliformes isolados de leite humano ordenhado

Novak, Franz Reis; Almeida, João Aprígio Guerra de; Asensi, Marise Dutra; Moraes, Bianca Aguiar de; Rodrigues, Dália dos Prazeres

doi:10.1590/s0102-311x2001000300026

Cited by 13 publications

(12 citation statements)

References 8 publications

(6 reference statements)

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“…Regarding the antibiotics used in the present study, a high prevalence of resistance to ampicillin and amoxicillin was observed. Resistance to ampicillin was also observed in a study on clinical isolates of K. pneumonia ( 27 ) and in a study on isolates of this enterobacterium in expressed human breast milk ( 28 ).…”

Section: Discussionmentioning

confidence: 72%

Potential virulence of Klebsiella sp. isolates from enteral diets

Pereira

Vanetti

2015

Braz J Med Biol Res

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We aimed to evaluate the potential virulence of Klebsiella isolates from enteral diets in hospitals, to support nosocomial infection control measures, especially among critical-care patients. Phenotypic determination of virulence factors, such as capsular expression on the external membrane, production of aerobactin siderophore, synthesis of capsular polysaccharide, hemolytic and phospholipase activity, and resistance to antibiotics, which are used therapeutically, were investigated in strains of Klebsiella pneumoniae and K. oxytoca. Modular industrialized enteral diets (30 samples) as used in two public hospitals were analyzed, and Klebsiella isolates were obtained from six (20%) of them. The hypermucoviscous phenotype was observed in one of the K. pneumoniae isolates (6.7%). Capsular serotypes K1 to K6 were present, namely K5 and K4. Under the conditions of this study, no aerobactin production, hemolytic activity or lecithinase activity was observed in the isolates. All isolates were resistant to amoxicillin and ampicillin and sensitive to cefetamet, imipenem, chloramphenicol, gentamicin and sulfamethoxazole-trimethoprim. Most K. pneumoniae isolates (6/7, 85.7%) from hospital B presented with a higher frequency of resistance to the antibiotics tested in this study, and multiple resistance to at least four antibiotics (3/8; 37.5%) compared with isolates from Hospital A. The variations observed in the antibiotic resistance profiles allowed us to classify the Klebsiella isolates as eight antibiotypes. No production of broad-spectrum β-lactamases was observed among the isolates. Our data favor the hypothesis that Klebsiella isolates from enteral diets are potential pathogens for nosocomial infections.

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Section: Discussionmentioning

confidence: 72%

Potential virulence of Klebsiella sp. isolates from enteral diets

Pereira

Vanetti

2015

Braz J Med Biol Res

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“…2 Research has indicated that contamination by coliform microorganisms may originate from the environment, as observed in a study in which this group of bacteria were isolated in 38.4% of a total of 472 samples 17 , and among a total 5,710 samples of human milk, coliforms were detected in 1,139 (19.9%) 17 and in a total of 837 samples, 71 (8.48%) were contaminated with coliform microorganisms, whereas only three of these were E. coli. 18 In this study, coliforms were present in 21.1% of the samples of raw milk and in 5.6% of the pasteurized milk samples, indicating that the efficiency of pasteurization may be compromised by an initially high count of microbes.…”

Section: Discussionmentioning

confidence: 58%

Qualidade microbiológica de leite humano obtido em banco de leite

et al. 2003

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ObjectiveThe objectives of the present study were to determine the prevalence of potentially pathogenic microorganisms that indicate the hygienic and sanitary conditions of human milk samples collected at a Human Milk Bank. MethodsThree hundred and thirty eight (338) samples of human milk collected from a milk bank in a maternity in the municipality of Goiânia, in the state of Goias, Brazil were submitted to microbiological analysis. The latter were plated on McConkey agar according to the type of bacteria. Among the total number of samples collected, 194 consisted of raw milk and the remaining 144 were pasteurized milk.

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“…Among the antibiotics used in the present study, it was seen that there was high prevalence of resistance to ampicillin and amoxicillin. This observation regarding the antibiotic ampicillin was also made in a study on clinical isolates of K. pneumonia [23] and in a study on isolates of this enterobacterium in expressed human breastmilk [24].…”

Section: Discussionmentioning

confidence: 68%

Potential Virulence of Klebsiella Isolated from Enteral DietsVirulence of Klebsiella and Enteral Diets

Pereira¹

2014

J Food Process Technol

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Objective: To evaluate the potential virulence of Klebsiella isolates from enteral diets in hospitals, in order to support nosocomial infection control measures, especially among critical patients. Methods: Capsular phenotypic expression of the external membrane, production of aerobactin siderophore, quantity of capsular polysaccharide, hemolytic and phospholipase activity and resistance to antibiotics that are used therapeutically were investigated in 15 strains of K. pneumoniae and six of K. oxytoca. These isolates were obtained from enteral diets in two public hospitals in the state of Minas Gerais, Brazil. Results: The hypermucoviscous phenotype was observed in one of the K. pneumoniae isolates (6.7%). Capsular serotypes of types K1 to K6 were seen to be present, of which four were K5 isolates of K. pneumoniae and one was K4. Under the conditions of this study, no aerobactin production, hemolytic activity or lecithinase activity was observed. All the isolates presented resistance to the antibiotics amoxicillin and ampicillin, but they were sensitive to the antibiotics cefetamet, imipenem, cloranfenicol, gentamicin and sulfamethoxazole/trimethoprim. The K. pneumoniae isolates that originated from hospital B presented higher frequency of resistance to the antibiotics evaluated and multiple resistances to at least four antibiotics. The variations in the profile of resistance to antibiotics among the Klebsiella isolates made it possible to classify them into eight antibiotypes. No production of broad-spectrum β-lactamases was observed among the isolates. Conclusion: The data obtained through this study favor the hypothesis that Klebsiella isolates from enteral diets are potential pathogens for nosocomial infections.

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Resistência antimicrobiana de coliformes isolados de leite humano ordenhado

Abstract: The dispersion of potentially pathogenic, antibiotic-resistant microorganisms via expressed human milk can be considered a risk factor. The aim of this study was to contribute to

Cited by 13 publications

References 8 publications

Potential virulence of Klebsiella sp. isolates from enteral diets

Potential virulence of Klebsiella sp. isolates from enteral diets

Qualidade microbiológica de leite humano obtido em banco de leite

Potential Virulence of Klebsiella Isolated from Enteral DietsVirulence of Klebsiella and Enteral Diets

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