Morphometry and histomorphometry of the testis in crossbred pigs fed diets with different protein levels

Valença, Rômulo Menna Barreto; Silva, V. A.; Araújo, L. P. C.; Reis, J. C. dos; Guerra, Maria Madalena Pessoa; Soares, Pierre Castro; Costa, A. N.

doi:10.1590/s0102-09352013000500010

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…Quantitative histological analysis was performed with 20 fields per testicular sample. For each field of view, the diameter of the seminiferous tubules, height of the spermatogenic epithelium, number of spermatogonia and the number of Sertoli cells were estimated (Bruveris et al, 2012; Valença et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Arginine promotes testicular development in boars through nitric oxide and putrescine

Wei

Che

et al. 2021

Animal Physiology Nutrition

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Background: The present work aimed to explore the in uence of arginine on testicular development in boars and the underlying mechanism. Methods: To this end, thirty 30-day-old male Duroc piglets (7.00 ± 0.30 kg) were randomly sorted into two groups maintained on either a basal diet (CON, n = 15) or a diet supplemented with 0.8% arginine (ARG, n = 15). The average daily gain, average daily feed intake, and testicular volume were determined periodically, and serum samples were collected once every 30 days. The amino acid composition and arginine metabolite levels were estimated in testes samples, collected by castration of three 150-day-old boars randomly selected from each group. Boars semen was collected at 240 days of age to estimate sperm production and quality.Results: The results showed that dietary supplementation of arginine had no effect on boar growth performance (P > 0.05) but increased the testicular volume and weight of 120-and 150-day-old boars, respectively (P < 0.05). Quantitative analysis of testicular histology showed a higher value for the number of spermatogonia and height of the seminiferous epithelium in the ARG group (P < 0.05). The sperm density, total number of sperm, and effective number of sperm of the boars in the ARG group increased signi cantly compared with the CON group (P < 0.05). Although arginine supplementation did not affect plasma amino acid levels, the testicular arginine levels in 150-day-old boars showed a signi cant increase (P < 0.05). The level of serum nitric oxide (NO) and activity of nitric oxide synthase (NOS) also increased in 150-day-old boars in the ARG group (P < 0.05). Interestingly, dietary supplementation of arginine increased the testicular levels of putrescine in 150-day-old boars (P < 0.05).Conclusions: These results indicate that arginine supplementation increases serum NO levels and testicular arginine and putrescine levels, thereby improving testicular development and semen quality in boars.

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Section: Methodsmentioning

confidence: 99%

Arginine promotes testicular development in boars through nitric oxide and putrescine

Wei

Che

et al. 2021

Animal Physiology Nutrition

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Age-related histomorphometric and ultrastructural changes in the Sertoli cells of Japanese quail (Coturnix Coturnix japonica)

et al. 2021

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<b>Evaluation of quantitative parameters of Leydig cell in diabetic adults rats

Silva

Oliveira

et al. 2014

Acta Sci. Biol. Sci.

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ABSTRACT. The goal of present study was to evaluate the effects of diabetes on quantitative parameters of Leydig cells. Twelve adults Wistar rats were divided in: 1) Diabetic Group (DG), which was induced by a single intraperitoneal injection of streptozotocin (60 mg kg -1 of body weight); and 2) Control Group (CG), which received citrate buffer intraperitoneal. After eight weeks of diabetic induction, the animals were weighted, anesthetized and testicles were removed and routinely processed to paraffin embedded. Body weight (40%) and testicular weight (18%) of diabetic rats were significantly lower than control group. Diabetic rats showed an increase in interstitial compartment but the tubular compartment did not differ. The individual volume of Leydig cells and nuclear diameter were lower in DG. However, the population of these cells was increased. In conclusion, diabetes induced by streptozotocin in adult rats promoted alterations in testicular compartments and changes on volume, nuclear diameter and population of Leydig cells, compromising the testicular function.Keywords: streptozotocin, testes, interstitial cells, morphometry. Avaliação de parâmetros quantitativos de células de Leydig em ratos diabéticos adultosRESUMO. O objetivo do presente estudo foi avaliar os efeitos do 'diabetes' nos parâmetros quantitativos de células de Leydig. Doze ratos machos adultos foram divididos em: 1) Grupo Diabético (GD) induzidos por injeção intraperitoneal única de estreptozotocina (60 mg kg -1 de peso corporal); e 2) Grupo Controle (GC) receberam tampão citrato, via intraperitoneal. Após oito semanas da indução, os animais foram pesados, anestesiados, os testículos foram removidos e processados rotineiramente em parafina. O peso corporal (40%) e testicular (18%) dos ratos diabéticos reduziu significativamente em relação ao grupo controle. Ratos diabéticos mostraram aumento no compartimento intersticial, mas o compartimento tubular não apresentou diferença significativa. O volume individual e o diâmetro nuclear de células de Leydig reduziram em GD. No entanto, a população dessas células aumentou. Em conclusão, o 'diabetes' induzido por estreptozotocina, em ratos adultos, promoveu alterações nos compartimentos testiculares e mudanças no volume, diâmetro nuclear e população das células de Leydig, comprometendo a função testicular.Palavras-chave: estreptozotocina, testículo, células intersticiais, morfometria.

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Morphometry and histomorphometry of the testis in crossbred pigs fed diets with different protein levels

Cited by 3 publications

References 9 publications

Arginine promotes testicular development in boars through nitric oxide and putrescine

Arginine promotes testicular development in boars through nitric oxide and putrescine

Age-related histomorphometric and ultrastructural changes in the Sertoli cells of Japanese quail (Coturnix Coturnix japonica)

<b>Evaluation of quantitative parameters of Leydig cell in diabetic adults rats

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