Characterization of mesenchymal stem cells derived from equine adipose tissue

Abstract

Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.

Conditioned medium: a new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells

“…Prior to cryopreservation, UCIM-MSCs were satisfactorily characterized with respect to morphology, plastic adherence, immunophenotypic profile, potential to differentiate into mesodermal lineages, clonicity, and chromosomal stability. These characteristics corroborate with some findings observed in other studies with UCIM-MSCs from umbilical cord (Hoynowski et al, 2007;Lange-Consiglio et al, 2011;Barberini et al, 2014), amniotic liquid (De , amniotic membrane (Lange-Consiglio et al, 2012), bone marrow (Maia et al, 2012(Maia et al, , 2015, adipose tissue (Carvalho et al, 2013b), and peripheral blood (Martinello et al, 2010;Carvalho et al, 2013a).…”

Expression patterns of mesenchymal stem cell-specific proteins in adipose tissue-derived cells: possible immunosuppressing agent in partial allograft for restoring the urinary bladder in rabbits

“…Finally, the cells need to be able to differentiate into bone, fat and cartilage (Bydlowski et al 2009). In the current study, cell adherence to plastic culture flasks as well as fibroblastoid cell morphology identified cells used for allografts as MSCs (Torres et al 2007, Bydlowski et al 2009, Monteiro et al 2010, Carvalho et al 2013, Xu et al 2015. Although have been identified eight surface markers for identifying MSCs, the International Society for Cellular Therapy agrees that the identification of markers CD105, CD73 and CD90, the absence of hematopoietic markers (CD45, for example), is sufficient for MSC immunophenotyping (Monteiro et al 2010), as performed in our study.…”

The potential therapeutic efficacy of intravenous versus subconjunctival mesenchymal stem cells on experimentally ultraviolet-induced corneal injury in adult male albino rats

“…The adipocytes differentiation was recognized by using adipocytes StemPro® adipogenesis differentiation kit (Gibco, Grand Island, NY, USA) and stained with Oil Red O stain (Sigma-Aldrich, St Louis, MO, USA). The chondrocytes differentiation was recognized by using chondrocytes StemPro® chondrogenesis differentiation kit (Gibco, Grand Island, NY, USA) and stained with Alcian Blue stain (Sigma-Aldrich) [7].…”

“…This was carried out by using Fluorescence-Activated Cell Sorting (FACS) (BD Biosciences, Franklin Lanes, NJ, USA) in which the cells were incubated at 4°C in the dark for 20 minutes with 2 μl fluorescein isothiocyanate (FITC)-conjugated rat monoclonal antibody against mouse CD90 and CD105 (BD Biosciences) and 5 μl phycoerythrin (PE)-conjugated rat monoclonal antibody against mouse CD45 (BD Biosciences). The samples were centrifuged at room temperature for 5 minutes at 300 × g, resuspended in 500 μl PBS then analysed by the flow cytometry [7].…”