Estudo retrospectivo de exames citológicos realizados em um Hospital Veterinário Escola em um período de cinco anos

Rosolem, Mayara Caroline; Moroz, Ludmila Rodrigues; Rodigheri, Sabrina Marin; Neto, Urias José correa; Porto, Camila Dias; Hanel, J. S

doi:10.1590/s0102-09352013000300019

Cited by 11 publications

(19 citation statements)

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“…In this study, the total number of inconclusive cases corresponded to 5.81% of all cases examined, being proportionally classifi ed as equal or lower when compared with the numbers of inconclusive cases reported by other authors [12][13].…”

Section: Discussionmentioning

confidence: 50%

Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis

et al. 2019

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The aim of this study was to evaluate the main nonconformities that result in cytopathological samples inappropriate for diagnosis in veterinary medicine. All cytopathological samples, obtained from different canine and feline tissues/lesions, included in the study were received and classified as inconclusive by a single public laboratory of veterinary pathology, located in Pernambuco State, Brazil, between 2012 and 2016. Nonconformities regarding the smear quality, cellularity, presence or absence of hemorrhage, cellular overlapping, desiccation, and presence or absence of necrotic debris and/or artifacts were evaluated. Data were tabulated using Microsoft Excel 2007; absolute and relative frequencies were calculated using EPIINFO 3.5.2. From the 3268 cases received between 2012 and 2016, 190 cases were selected and comprised 514 inconclusive slides. The most frequent nonconformities detected were insufficient/absence of cellularity in 100% (514/514), inadequate submacroscopic presentation in 87% (446/514), and hemorrhage in 69% (356/514) of samples. Other features identified were cellular overlapping in 34% (175/514), inadequate staining in 31% (175/514), artifacts in 30% (154/514), desiccation in 28% (145/514), and necrotic debris in 26% (133/514) of samples. The implementation of laboratory standard operational procedures aimed at maintaining quality is essential. It is necessary to initially identify the main errors occurring in the processing stages as a way to guide and design strategies to avoid them.

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Section: Discussionmentioning

confidence: 50%

Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis

et al. 2019

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“…e Dirofilaria immitis. A literatura corrobora a importância da citologia por PAAF na detecção de agentes em lesões diversas sem suspeita clínica das enfermidades por eles causadas [23, 31,34,36]. Nesse contexto, pesquisadores [40] igualmente encontraram, de forma incidental, microfilárias de D. immitis em análise citológica de um aumento de volume na região do antebraço de um cão sem qualquer sintomatologia relacionada à parasitose, enfatizando a importância de sempre utilizar esta ferramenta diagnóstica na rotina clínica de animais com lesões tumorais.…”

Section: Discussionunclassified

Presence of Leishmania sp. and Dirofilaria immitis in Cutaneous Canine Transmissible Venereal Tumor

Rodrigues

Borges

Dantas

et al. 2019

Acta Scientiae. Vet.

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Background: Canine Transmissible Venereal Tumor (CTVT) is a neoplasm transmitted by implantation of its cells into genital and extragenital organs, while Heartworm and Visceral Leishmaniasis are zoonosis transmitted by hematophagous insects that are often underdiagnosed in asymptomatic animals. Coinfection by the agents of these parasitosis is well documented, however, the association of both diseases with the CTVT is still unusual. Thus, it was aimed to report a case of incidental identification of microfilariae of D. immitis and amastigotes forms of Leishmania sp. in cutaneous CTVT by cytology in an asymptomatic dog for the parasitosis.Case: A 6-year-old, male, mongrel, sexually intact dog from the city of Patos, Paraiba, Brazil, was presented with a cutaneous circumscribed tumoral lesion in a region adjacent to the right olecranon, with an ulcerated surface of reddish color, having areas of necrosis inside and larvae (myiasis), draining bloody secretion. The material was collected for cytological analysis, which proved to be Canine Transmissible Venereal Tumor (CTVT) with the presence of amastigote forms of Leishmania sp. and microfilariae of D. immitis among neoplastic cells. Blood count, serum urea, creatinine and albumin, ALT, AST, FA, CK, Na+, K+, Ca++, CK-MB, Troponin I (cTnI), Snap 4Dx Plus (Idexx®), Snap Leishmania (Idexx®), and Knott's test were performed, plus chest radiography, blood pressure measurements and electrocardiogram (ECG). The alterations found corresponded to normochromic normocytic anemia, thrombocytopenia, hypoalbuminemia, microfilariae in the Knott test, increase of CK, CK-MB and cTnI, positivity for Erlichia sp., Anaplasma sp., Leishmania sp. and Dirofilaria immitis. Furthermore cardiomegaly on radiographs and sinus arrhythmia associated with atrioventricular block (AVB) of the first degree on the ECG. Euthanasia was performed after necropsy, in which adult worms were observed in the right heart chambers.Discussion: The coexistence of neoplastic diseases and infectious microorganisms is often detected only incidentally in asymptomatic animals and complementary tests are important tools to support clinical suspicions, as was observed in the present case. The fact that the animal is not neutered, be in full sexual activity and have unrestricted life habit, favored his involvement by CTVT due to greater contact with other whole canines. Coinfection by Dirofilaria sp. and Leishmania sp., identified in this case, has already been confirmed in several countries, suggesting the presence of distinct vectors in the same geographical area and the imminent exposure of humans to these agents. Despite the studies evidencing the systemic infection by the two parasites, there are no reports of mutual parasitism by the pathogens mentioned in canine neoplasms, especially in the cutaneous extragenital form of the CTVT, making the present case uncommon. The cytological examination was fundamental not only to show that the cutaneous lesion was a CTVT, but also to have evidenced the parasites Leishmania sp. and Dirofilaria immitis, whose infections remained hidden. The changes present in hematology are commonly present in the diseases diagnosed in this case, and elevation of CK, CK-MB and cTnI denote damage to cardiomyocytes due to the action of both parasites. Cardiomegaly on radiographs and 1st degree AVB on the ECG also correspond to secondary changes in the presence of worms in the animal's cardiovascular system. Thus, the presence of a dog autochthonously infected by parasites with a zoonotic potential reveals the susceptibility of other animals and humans in the region to infections.

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“…É uma técnica minimamente invasiva, apresenta segura execução, além de proporcionar ampla superfície de amostragem e rápido tempo entre o processo de diagnóstico e o resultado (Magalhães et al, 2014). Baseia-se na análise da célula de forma individual, fornecendo informações relacionadas a sua origem, permitindo, por exemplo, a identificação e diferenciação de inflamação, hiperplasia, infecções ou a presença de tumores sejam eles cancerígenos ou não, determinando o prognóstico do animal, a presença de metástases e evolução/involução do quadro clínico (Rosolem et al, 2013). A obtenção de resultados clinicamente relevantes depende, dentre outros fatores, da colheita de boas amostras contendo áreas representativas da lesão, sem artefatos ou contaminação, corados por métodos que evidenciem tanto critérios citoplasmáticos quanto critérios nucleares, levando em consideração a espécie animal, o histórico clínico e descrição macroscópica das lesões (Coleto et al, 2016).…”

Section: Introductionunclassified

Utilização do exame citológico no diagnóstico de afecções de cães e gatos

Oliveira

Rodrigues

Santos

et al. 2021

RSD

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Este trabalho verificou a utilização do diagnóstico citológico, como método de identificação de afecções em cães e gatos domiciliados no município de Barra-BA atendidos no Hospital Veterinário Universitário (HVU) da Universidade Federal do Oeste da Bahia (UFOB). O estudo foi realizado a partir da análise de amostras citológicas relativas aos casos clínicos atendidos nos anos de 2018 e 2019. Foram atendidos 711 animais, desses contabilizadas 105 (101 caninos e 4 felinos - 59 fêmeas e 46 machos) solicitações do exame citológico. A maioria dos animais (28,57%) apresentavam de 6 a 10 anos, prevalecendo os animais Sem Raça Definida (62,38%). Os processos inflamatórios foram diagnosticados em 43,80% dos casos, identificados como de causa infecciosa em 86,90% (30% por Leishmania spp., 27,5% infecções bacterianas, 27,5% pela levedura Malassezia spp. e outros 15% por associação deste fungo a bactérias). Os processos inflamatórios de origem não infecciosa foram registrados em 13,10% casos (84% infiltrados de células inflamatórias e 16% como dermatite por lambedura). Observou-se processos não inflamatórios em 19,05% das amostras, sendo 95% de origem neoplásica, (63,15% TVT, 21,05% Carcinoma de Células Escamosas, 10,05% Adenocarcinoma e 5,30% Tricoblastoma). Já entre os processos não inflamatórios e não neoplásicos (5%) diagnosticou-se um cisto epidérmico (100%). A aplicabilidade do diagnóstico citológico em cães e gatos denota importância uma vez que, auxiliou médicos veterinários na confirmação de suspeitas clínicas, permitindo a emissão de laudos diagnósticos, estabelecimento de tratamentos e dados epidemiológicos que possibilitam a implantação de medidas de controle das enfermidades.

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Estudo retrospectivo de exames citológicos realizados em um Hospital Veterinário Escola em um período de cinco anos

Cited by 11 publications

References 5 publications

Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis

Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis

Presence of Leishmania sp. and Dirofilaria immitis in Cutaneous Canine Transmissible Venereal Tumor

Utilização do exame citológico no diagnóstico de afecções de cães e gatos

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