2013
DOI: 10.1590/s0102-09352013000300010
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Abstract: After a serious injury or sudden death, epididymis cauda sperm recovery and cryopreservation may present as the last opportunity to obtain genetic material from a valuable stallion. This study evaluated the viability of cooled equine sperm collected by three different methods: sperm of ejaculated (G1), sperm recovered from the epididymal cauda immediately after orchiectomy (G2) and sperm recovered from the epididymal cauda after storage for 24 hours at 5°C (G3). To obtain G1 sperm, two ejaculates were collecte… Show more

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Cited by 6 publications
(4 citation statements)
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References 16 publications
(11 reference statements)
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“…A study demonstrated that epididymal semen has superior cooling ability than ejaculated stallion semen; this does not seem to be the case in donkeys [32]. While the present study did not compare donkey ejaculated sperm with donkey epididymal semen, the overall results of the semen parameters appear inferior to that expected with cooled ejaculated donkey semen [2].…”
Section: Discussioncontrasting
confidence: 79%
See 1 more Smart Citation
“…A study demonstrated that epididymal semen has superior cooling ability than ejaculated stallion semen; this does not seem to be the case in donkeys [32]. While the present study did not compare donkey ejaculated sperm with donkey epididymal semen, the overall results of the semen parameters appear inferior to that expected with cooled ejaculated donkey semen [2].…”
Section: Discussioncontrasting
confidence: 79%
“…Across mammalian species, sperm stored at the tail of the epididymis is immotile due to plasma membrane-bound proteins that prevent premature motility and loss of viability [33,34]. In stallions, the EY extender used in the present study was shown to activate the motility of epididymal semen [8]; thus, the protocol of directly harvesting epididymal semen with this extender became popular in equine clinical practice and has been used in numerous investigations [6,32]. Semen centrifugation has been used as a means to potentially activate epididymal sperm motility in mice by removing plasma membrane-bound proteins [35].…”
Section: Discussionmentioning
confidence: 88%
“…Contact between spermatozoa and seminal plasma components is known to result in biochemical changes and plasmatic membrane damages, compromising the quality of frozen–thawed sperm (Monteiro et al ., 2013). In fact, it is known that peccaries’ seminal plasma contains proteins involved in spermatic protection, such as clusterin and T-complex protein 1, and proteins with antioxidant activities, like glutathione peroxidase (GPx-5), ceruloplasmin, albumin, and transferrin (Santos et al , 2014).…”
Section: Discussionmentioning
confidence: 99%
“…There is limited data regarding differences between the quality of epididymal and ejaculated spermatozoa in other marsupials, although the methodology has been outlined for several other mammalian species. While significant differences were found between the motility of ejaculated and cauda epididymal sperm in the Holstein bull (Stout et al, 2012) and the ram (White and Wales, 1961), in some species, such as the donkey (Gloria et al, 2011) and the stallion (Monteiro et al, 2013), the epididymal spermatozoa were surprisingly better than the ejaculated samples. Those studies on domestic species associated the difference between epididymal and ejaculated sperm performance to the high levels of minerals in the ejaculate (such as sodium and potassium), which were not present in the epididymal samples.…”
Section: Discussionmentioning
confidence: 85%