2013
DOI: 10.1590/s0102-09352013000100009
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Abstract: The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 strain and samples of semen and urine were collected every week up to 180 days post infection. Out of 83 semen samples col… Show more

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Cited by 6 publications
(4 citation statements)
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“…While B. ovis is considered non pathogenic for humans, B. melitensis has the highest zoonotic potencial among all Brucella species [23]. Furthermore, flocks identified as positive for B. ovis using the multiplex PCR method developed in this study, can be further investigated by using a more sensitive species-specific nested PCR method that has been recently developed [24]. …”
Section: Discussionmentioning
confidence: 99%
“…While B. ovis is considered non pathogenic for humans, B. melitensis has the highest zoonotic potencial among all Brucella species [23]. Furthermore, flocks identified as positive for B. ovis using the multiplex PCR method developed in this study, can be further investigated by using a more sensitive species-specific nested PCR method that has been recently developed [24]. …”
Section: Discussionmentioning
confidence: 99%
“…These studies showed that the use of nested primers gave increased sensitivity and higher specificity providing a better molecular diagnostic approach for human brucellosis. Costa et al, (2013) evaluate species-specific nested PCR based on a previously described (Xavier et al, 2010) species-specific PCR assay for detection of Brucella ovis in semen and urine samples of experimentally infected rams. The results showed that performance of the species-specific nested PCR was significantly more sensitive as compare to genus-specific PCR.…”
Section: Nested and Semi-nested Pcrmentioning
confidence: 99%
“…Diagnosis of B. ovis infections is usually based on clinical examination, serology and bacterial isolation from semen samples [ 5 ]. Although experimental studies demonstrated that B. ovis can be equally detected in urine and semen samples by PCR or nested PCR [ 6 , 7 ].…”
Section: Introductionmentioning
confidence: 99%