Parameters for evaluation of clinical trial in mice infected with Trypanosoma cruzi

Abstract

The effectiveness of clinical parameters in the evaluation of Trypanosoma cruzi infection was analyzed in male Swiss mice at 8 weeks old Animals were divided into HG (healthy) and IG (1400 trypomastigotes, intraperitoneally, Y strain -Trypanosoma cruzi). Quantitative and qualitative parameters were evaluated in non-consecutive days in the period, from 7 th -11 th and 15 th -18 th days of infection. There were significant differences (P<0.05) between both groups in both periods regarding water consumption, abdominal circumference and weight. The second group presented differences in amount of excreta, body temperature, move-up and mortality. There was no difference (P>0.05) between the groups in food consumption, exploration of self-cleaning and skin staining. The fecal feature differed between the groups in the second period. The occurrence of isolation was not practical. Differences were observed in the hair between groups, although the parameter had been interfered by fights between animals. The consumption of water, feed, excreta production, characteristic of the faeces, body temperature, abdominal circumference, move up, weight and mortality parameters are easy to be measured and effective in clinical differentiation of healthy mice infected with T. cruzi, elected in protocols for clinical study with mice, which is the first work to gather information of qualitative and quantitative clinical parameters evaluated in these animals. Keywords: clinical analysis, Chagas disease, experimental infection, mice, Trypanosoma cruzi RESUMO Analisou-se a eficiência de parâmetros clínicos na avaliação da infecção pelo Trypanosoma cruzi em camundongos suíços, machos de 8 semanas. Os grupos foram divididos em GS (sadios) e GI (1400 tripomastigotas, intraperitoneal, cepa Y -Trypanosoma cruzi

Different treatment schemes and dynamizations of Trypanosoma cruzi biotherapies: what information do they transfer to the organism in infected mice?

“…In contrast, when administered 3 days before infection and 3 days after infection (TBA 7dH ), the animals had lower parasitemia and a longer survival curve compared with the control group, reflected by a better clinical evolution (i.e., stable body temperature, higher body weight gain, and higher food and water intake compared with the control group) on day 11e28 after infection, a period of high morbidity when animals usually start to present a reaction to the infection. 25 In this experimental model (Swiss mice are highly susceptible to infection), the evolution of the disease is irreversible, culminating in the death of untreated animals (IC group). 28 Thus, an increase in the survival period, a decrease in parasitemia, and better clinical evolution in the host are indicators of a good treatment scheme, independent of the presence of the parasite.…”

“…The animals were clinically evaluated 3 days before infection and on days 0, 4,8,11,15,18,21,25, and 28 after infection using a standard schedule. Body mass (g) was measured using a semi-analytical balance (BEL Engineering, Class Mark II).…”

Associação de medicamentos homeopáticos promove diminuição da parasitemia e melhora clínica em camundongos infectados por Trypanosoma cruzi

“…Os animais foram divididos em quatro grupos, mantidos em gaiolas e avaliados durante cinco dias antes da infecção, no dia da infecção e 27 dias consecutivos após a infecção em horário fixo (14). Os seguintes parâmetros foram avaliados: Pesoexpresso em grama (g), avaliado individualmente em balança digital BEL engineering ® -Class Mark II 500g; Temperaturaexpressa em grau centigrado (ͦ C), medida individualmente na região anterior da coxa traseira esquerda (menor quantidade de pelos), utilizando termômetro infravermelho digital Icel (modelo TD-920.0387); Consumos de água e raçãoexpressos em mililitro (mL) e grama (g), respectivamente.…”

Zincum metallicum 5cH increases survival and improves clinical mice infected with Trypanosoma cruzi

“…Treatment was diluted in water (1mL/100mL). Clinical (temperature, weight, water/foodintake and excreta) [2] and parasitological parameters (pre-patent and patent period, peak parasitemia, and parasitemia overall survival time) [3] were assessed daily. Data were compared BioEstat 5.0, significance level of 5 %.…”